Photonic and phononic crystals are metamaterials with repeating unit cells that result in internal resonances leading to a range of wave guiding and filtering properties and are opening up new applications such as hyperlenses and superabsorbers. Here we show the first, to our knowledge, 3D colloidal phononic crystal that is reconfigurable in real time and demonstrate its ability to rapidly alter its frequency filtering characteristics. Our reconfigurable material is assembled from microspheres in aqueous solution, trapped with acoustic radiation forces. The acoustic radiation force is governed by an energy landscape, determined by an applied high-amplitude acoustic standing wave field, in which particles move swiftly to energy minima. This creates a colloidal crystal of several milliliters in volume with spheres arranged in an orthorhombic lattice in which the acoustic wavelength is used to control the lattice spacing. Transmission acoustic spectroscopy shows that the new colloidal crystal behaves as a phononic metamaterial and exhibits clear band-pass and band-stop frequencies which are adjusted in real time.reconfigurable acoustic assembly | acoustic metamaterials | aqueous suspension of microspheres A rtificially engineered metamaterials have attracted significant research interest due to their useful wave guiding and transmission properties. The interest in these materials stems from the possibility of gaining previously unheralded control over wave phenomena, for example, controlling the path of waves leads to incredibly efficient lenses (1) or invisibility cloaking (2, 3) and controlling their transmission and reflection leads to highly efficient filters (4), diodes (5-7), or superabsorbers (8).Photonic and phononic crystals are periodic metamaterials typically created from multiple elementary repeating cells. They exhibit a well-known series of band-pass and band-stop frequencies that have attracted significant attention for use as filters and absorbers. Here we demonstrate a phononic crystal reconfigurable in real time, although the same principles could be used to fabricate other metamaterials including photonic crystals, as well as the more elaborate configurations required for applications such as cloaking.The frequencies of the band gaps in phononic crystals can be tuned by changing the lattice geometry (9), and the width of the gaps depends on the contrast between the densities and sound velocities of the component materials. Most of the work to date has not involved any reconfigurability, for example, 2D phononic crystals with a periodicity millimeter order and above have been assembled manually and used to explore their basic properties (10, 11). A reconfigurable phononic crystal has been created in 2D using optical tweezers but this is limited to a relatively small scale (' 1 × 10 8 -m 2 area) and to transparent or dielectric particles (12). Three-dimensional experimental realizations of phononic crystals have either been manufactured with millimeter periodicity or as colloids (13,14). Colloids r...