Orchid Mycorrhizal Fungi: Structure, Function, and Diversity

Sathiyadash, Kullaiyan; Muthukumar, Thangavelu; Karthikeyan, Velusamy; Rajendran, K.

doi:10.1007/978-981-32-9456-1_13

Cited by 20 publications

(18 citation statements)

References 170 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While the association of C. radians to terrestrial orchids is not new (Terashita & Chuman 1987, Yagame et al 2013, Yamato et al 2005, Yukawa et al 2009, our results report for the first time the association of C. radians to epiphytic orchids and reveal a potential ecological role for this endophyte. Saprophytic members of Coprinaceae have been reported as potential intermediary providers of organic carbon from decaying wood to the mycothropic orchid Epipogium roseum (Sathiyadash et al 2020). Carbon remobilized by fungi from decaying substrates could serve as energy source for germinating orchid seeds and favor the establishment of orchid-endophyte associations.…”

Section: Discussionmentioning

confidence: 99%

“…Under natural conditions, orchid seeds depend on their interactions with a variety of fungi such as Ceratobasidium spp., Tulasnella spp., Rhizoctonia spp., Epulorhiza spp., and Ceratorhiza spp. to obtain phosphorus, carbon, and nitrogen from the environment to support germination and initial development (Rasmussen 1995, Sathiyadash et al 2020, Smith & Read 2010. Understanding these orchid-fungus interactions is key to supporting conservation efforts because orchids produced using alternative propagation methods, such as in vitro culture, show low survival rates after re-introduction into native or rehabilitated habitats and slow growth thereafter (Chen, Wang & Guo 2012, Swarts & Dixon 2009.…”

mentioning

confidence: 99%

“…Understanding these orchid-fungus interactions is key to supporting conservation efforts because orchids produced using alternative propagation methods, such as in vitro culture, show low survival rates after re-introduction into native or rehabilitated habitats and slow growth thereafter (Chen, Wang & Guo 2012, Swarts & Dixon 2009. Members of Tulasnellaceae and Ceratobasidiaceae are among the most frequently reported mycorrhizal fungi associated with roots of epiphytic orchids (Sathiyadash et al 2020, Suárez et al 2006, and thus, have received most of the attention in orchid germination studies. However, orchid seed germination in nature likely involves other, and perhaps more complex, interactions with non-mycorrhizal fungi (Meng et al 2019), and even other types of organisms (Rasmussen et al 2015).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

et al. 2020

View full text Add to dashboard Cite

Orchids are known to establish complex relationships with endophytic fungi throughout their life cycle, and particularly during germination of their reserves-deprived seeds. Characterizing generalist or specialist interactions between orchids and associated fungi is key to supporting orchid conservation efforts. Here, endophytic fungi associated with roots of epiphytic orchids were studied in two montane Andean forests in Southern Ecuador. Orchid root samples were collected from ten ~500 m2 plots distributed between two neighboring forests. Endophytic fungi associated with these roots were then isolated, cultured, and identified by sequencing of rDNA markers. In total, 52 pure isolates were recovered from the roots of 10 orchid species. These isolates were classified into nine taxonomic groups except for one isolate that remained unclassified. Most fungal isolates were found in roots of up to two different orchid species; however, Coprinellus radians was found in the roots of all sampled orchids. The potential of C. radians to promote germination of orchid seeds was tested in a separate assay using seeds from two orchid species different than those found in the experimental forest plots. Of the two C. radians isolates tested, one improved germination in the two orchids evaluated to a level about half of that observed in seeds germinated in nutrient-rich medium (Phytamax) and above the null germination observed in plates without the fungus. Together, these results revealed a generalist relationship between C. radians and all the studied epiphytic orchids and the potential role of this fungus as a promoter of orchid seed germination. Key Words: Agaricales, Epidendrum, symbiotic germination

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The orchid may have fine-tuned its physiology to adapt to this fungus, thereby making the plants incapable of host-jumping for distantly related fungi (Bidartondo and Bruns, 2002), especially under in situ conditions (Masuhara and Katsuya, 1994;Perkins et al, 1995). Some sOMF and rOMF can promote orchid seed germination under controlled laboratory conditions, i.e., in vitro, thereby exhibiting potential specificity (Smith and Read, 2008;Rasmussen et al, 2015;Jacquemyn et al, 2017;Shao et al, 2019;Sathiyadash et al, 2020). If those isolates exhibiting potential specificity can successfully stimulate orchid seed symbiotic germination and survival under in situ conditions, they are considered to have ecological specificity.…”

Section: Seed Germination Promoting Orchid Mycorrhizal Fungi (Sgomf) Play Key Roles In the Orchid Reintroductionmentioning

confidence: 99%

Orchid Reintroduction Based on Seed Germination-Promoting Mycorrhizal Fungi Derived From Protocorms or Seedlings

Selosse

Luo

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Orchids are among the most endangered in the plant kingdom. Lack of endosperm in their seeds renders orchids to depend on nutrients provided by orchid mycorrhizal fungi (OMF) for seed germination and seedling formation in the wild. OMF that parasitize in germination seeds is an essential element for orchid seedling formation, which can also help orchid reintroduction. Considering the limitations of the previous orchid reintroduction technology based on seed germination-promoting OMF (sgOMF) sourced from orchid roots, an innovative approach is proposed here in which orchid seeds are directly co-sown with sgOMF carrying ecological specificity from protocorms/seedlings. Based on this principle, an integrative and practical procedure concerning related ecological factors is further raised for re-constructing long-term and self-sustained orchid populations. We believe that this new approach will benefit the reintroduction of endangered orchids in nature.

show abstract

“…Colonization of D. officinale roots by Mycena sp. increased plant height, biomass, and the number of shoots (Sathiyadash et al 2020). Epulorhiza sp., Mycena sp., Tulasnellales, Sebacinales, Cantharellales increased nutrient intake in plants and increased seed germination on D. nobile and D. chrysanthum.…”

Section: Mycorrhiza Roles On Seed Germination and Growth Of Dendrobiummentioning

confidence: 97%

Important Role of Mycorrhiza for Seed Germination and Growth of <i>Dendrobium</i> Orchids

Tsulsiyah

Farida

Sutra

et al. 2021

J.Tropical Biodiversity Biotechnology

View full text Add to dashboard Cite

Indonesia is a tropical country that has natural forests and is suitable for orchid species habitat, leading to more than 5,000 species of orchids grow. The tropical area is the main distribution centre for epiphytic orchids, one of which is Dendrobium, which grows more than 1,000 species throughout the world. Orchid seeds are very small and do not have an endosperm, making germination difficult in their natural habitat. Mycorrhizal association with orchids plays a role in the survival of orchids in nature through seed germination and growth. This study aims to provide a deeper understanding about the important role of mycorrhiza in seed germination and growth of Dendrobium. The mechanism of mycorrhizal association with orchids begins with the initial contact of the fungus with the orchid, hyphae enter the cortex cells to form peloton, peloton lysis, and exchange of nutrients occurs. Orchid mycorrhiza that mostly found groups in Dendrobium are Rhizoctonia (Epulorhiza, Tulasnella, Rhizoctonia). Mycorrhiza plays a role in increased secretion of phytohormone and enzyme activity which supports seed germination and growth of orchids. Specific mycorrhizal data on orchids can be used as an effort for in-situ and ex-situ conservation of Indonesian orchids, including Dendrobium.

show abstract

Orchid Mycorrhizal Fungi: Structure, Function, and Diversity

Cited by 20 publications

References 170 publications

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

Endophytic fungi associated with roots of epiphytic orchids in two Andean forests in Southern Ecuador and their role in germination

Orchid Reintroduction Based on Seed Germination-Promoting Mycorrhizal Fungi Derived From Protocorms or Seedlings

Important Role of Mycorrhiza for Seed Germination and Growth of <i>Dendrobium</i> Orchids

Contact Info

Product

Resources

About