Orchid Biotechnology

Vendrame, Wagner A.; Khoddamzadeh, Amir Ali

doi:10.1002/9781119281269.ch4

Cited by 8 publications

(6 citation statements)

References 209 publications

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“…Biotechnology offers two techniques for the conservation of plant species, including in vitro conservation and cryopreservation (Vendrame & Khoddamzadeh 2017). Through cryopreservation, long-term seed banks can be created, which represent one of the pillars of ex situ biodiversity conservation (Seaton et al 2010) and a promising alternative to other conservation approaches (Meritt et al 2014;Popova et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas

et al. 2021

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The objective of this study was to evaluate the efficiency of cryoprotective solution (PVS2) combined with phloroglucinol for the cryopreservation of seeds of two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds of Encyclia cordigera had 91.03% initial viability and 91.99% germination. The treatment of the seeds with PVS2 at 0 °C with 1% phloroglucinol for 60 min returned 93.79% viability and 91.01% germination after recovery from LN, consequently resulting in faster development of protocorms. For Epidendrum ciliare, seed viability was 85.65% and germination was 85.90%. Seed exposure to the PVS2 at 0 °C with 1% phloroglucinol for 180 min showed viability of 39.23% and germination of 37.88%. Despite lower germination, 78.90% of the protocorms reached stage P3 of development, when evaluated 45 days after sowing, not significantly different from the control 1, and showed normal development. These results indicate that PVS2 cryoprotective solution is efficient when combined with phloroglucinol for the cryopreservation and successful recovery of seeds of Encyclia cordigera and Epidendrum ciliare. The present study also indicates that response to cryopreservation and success of recovery after cold storage is species-specific and requires adjustments in exposure time to PVS2 at 0 °C prior to immersion in LN.

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Section: Introductionmentioning

confidence: 99%

“…The use of cryopreservation for the long-term seed storage, in a seed bank, consists in storing plant material in liquid nitrogen at -196 °C, or in the vapor phase of nitrogen at around -150 °C, for long time span and with a low risk of genetic or physiological variation (Reed 2008;Vendrame & Khoddamzadeh 2017).…”

Section: Introductionmentioning

confidence: 99%

Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas

et al. 2021

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“…In the recent years, several reports on micropropagation of Phalaenopsis orchids show that selection of suitable explants are the critical factors for the success of plant tissue culture (Vasil et al 2008, Vendrame andKhoddamzadeh et al 2016). Composition of media also significantly affect the induction, number and plant regeneration efficiency in Phalaenopsis.…”

Section: Introductionmentioning

confidence: 99%

“…(Kosiret et al (2004) has been reported six medium compositions for direct shoot regeneration of Phalaenopsis. Plant regeneration in many genera has been achieved through flower stalks bud, stem nodes (Balilashaki et al 2014), leaf tissues (Mayer et al 2010, Niknejad et al 2011, Vendrame and Khoddamzadeh 2016, shoot tips (Pant and Thapa et al 2012). Sinha et al (2010) studied the vegetative propagation of Phalaenopsis using young leaf segments on gelrite-gelled half strength MS supplemented with 2% sucrose, 2.0 mg/l BA, 0.5 mg/l NAA, 10% coconut water (CW), 2 g/l peptone and 1 g/l activated charcoal, the protocorm like bodies (PLBs) were induced within 12 weeks of culture.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Balilashaki

Zakizadeh

Olfati

et al. 2019

Plant Tissue Cult. & Biotech.

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With recent advances in high-throughput sequencing (HTS) technologies to improve plants, there is a need to release orchid specific genomic resources and platforms that are crucial for managing omics elements in systematic manner. Authors provide details about the recent developments in biotechnological techniques, genomics, transcriptomics, proteomics, metabolomics and their applications for the industrial production, propagation, conservation and manipulation of Phalaenopsis orchid. Plant Tissue Cult. & Biotech. 29(1): 133-149, 2019 (June)

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“…One of the most widely used auxins is NAA. Auxins aid in the process of cell growth and expansion (Vendrame and Khoddamzadeh, 2016), whereas another important PGR widely used is BAP, which is responsible for cell division. There have been many studies showing the importance of growth regulators in the in vitro micropropagation of orchids, such as Bautista-Aguilar et al (2021) in Vanilla planifolia, Do et al (2019) in Paphiopedilum hybrids, Huang and Fang (2021) in Cymbidium goeringii, Mi et al (2021) in Bletillastriata, Chookoh et al (2019) in Tolumnia, andDe Stefano et al (2022) in Epidendrum nocturnum.…”

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confidence: 99%

Conservation Horticulture: In Vitro Micropropagation and Acclimatization of Selected Florida Native Orchids

Mullin

Costa

Downing

et al. 2022

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Florida is home to 106 native orchid species, of which 77 are listed as endangered or threatened by the State of Florida. The Institute for Regional Conservation (IRC) has classified 62 of these species as either critically imperiled, imperiled, or rare in South Florida. Because of lack of endosperm, orchid germination rates are very low in nature, as they depend on an obligate relationship with mycorrhizal fungi for nutrients. Most orchid seeds can be germinated in vitro without the need for specific mycorrhizal fungi. This study aims are to establish a fast and efficient protocol for in vitro seed germination using different nutrient media and plant growth regulator (PGR) combinations, and to optimize seedling acclimatization protocols using different greenhouse media. To determine germination preferences, three different in vitro seed germination media were tested supplemented with PGRs, including 1) Murashige & Skoog (MS) (control), 2) MS supplemented with 1.5 mg/L 6-benzylaminopurine (BAP), and 3) MS supplemented with 1 mg/L BAP and 0.5 mg/L 1-Naphthaleneacetic acid (NAA) on Cyrtopodium punctatum, a state-listed endangered, IRC critically imperiled epiphytic orchid. There was no significant difference amongst the treatments after 2 and 6 weeks of seed sowing culture. To understand post-culture survivorship, two epiphytic and one terrestrial native species (Trichocentrum undulatum, Encyclia tampensis, and Oncidium ensatum) were chosen for the acclimatization study with two commercially available potting substrates (coir, sphagnum). To measure seedling growth rates, phenotypic measurements [leaf number, leaf length, root length, plantlet height, light intensity, pH, and electrical conductivity (EC)] and Soil Plant Analysis Development (SPAD) and Normalized Difference Vegetation Index (NDVI) values were recorded monthly for five months. All media/PGR combinations resulted in an initial high percentage of stage I growth but inhibited Protocorm-like bodies (PLBs) development, suggesting more research is necessary to determine later improvements or detriments to MS basal media with BAP and NAA. Comparing acclimatization media with the three species of orchid chosen for this experiment, neither O. ensatum nor E. tampensis showed a marked preference for sphagnum moss or coir. However, T. undulatum did perform better with coir compared with sphagnum. This research will help botanical gardens and commercial plant tissue culture laboratories to have a better understanding on selection of PGR combinations for in vitro cell culture and acclimatization media on increasing the viability and plant health and decreasing the mortality of endangered plants.

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Orchid Biotechnology

Cited by 8 publications

References 209 publications

Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas

Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Conservation Horticulture: In Vitro Micropropagation and Acclimatization of Selected Florida Native Orchids

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