Orchestration of the expression of the laccase multigene family in white-rot basidiomycete Trametes hirsuta 072: Evidences of transcription level subfunctionalization

Moiseenko, Konstantin V.; Vasina, Daria V.; Farukshina, Katerina T.; Savinova, Оlga S.; Glazunova, Olga A.; Федорова, Т.В.; Tyazhelova, T. V.

doi:10.1016/j.funbio.2018.02.006

Cited by 20 publications

(34 citation statements)

References 55 publications

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“…A detailed analysis of the subclades formed by the laccase genes from these fungi (Figure 2 and Supplementary Figure S2) revealed that the beginning of these clade proliferation losses and duplications of laccase genes were relatively uncommon events: in subclade A , neither gene losses nor duplications were detected; in subclade BE , one gene duplication in the common ancestor of the fungi from Sp 3 was detected; in subclade F , one gene loss in the lineage going to P. puniceus was detected; in subclade G , one gene loss in the common ancestor of the fungi from Sp 4 was detected; and in subclade H , two gene losses in the lineages going to T. hirsuta and T. gibbosa were detected. Interestingly, in the genome of T. hirsuta , the lost gene, lacH , is still readily detectable in the form of a pseudogene (Moiseenko et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…It is obvious that almost all native laccase proteins that were described in the literature until now belong to group A . These laccases are natively produced by the fungi of the CorePol_Clade under great varieties of growth conditions, and their transcriptional level is relatively stable during all cultivation stages (Moiseenko et al, 2018). In contrast, the native production of laccases from other groups was always observed under very specific inductive conditions such as induction by 2,5-xylidine for group BE laccases from T. villosa (Yaver et al, 1996); cocultivation with Trichoderma sp.…”

Section: Resultsmentioning

confidence: 99%

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Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

et al. 2019

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Laccase is one of the oldest known and intensively studied fungal enzymes capable of oxidizing recalcitrant lignin-resembling phenolic compounds. It is currently well established that fungal genomes almost always contain several non-allelic copies of laccase genes (laccase multigene families); nevertheless, many aspects of laccase multigenicity, for example, their precise biological functions or evolutionary relationships, are mostly unknown. Here, we present a detailed evolutionary analysis of the sensu stricto laccase genes (CAZy – AA1_1) from fungi of the Polyporales order. The conducted analysis provides a better understanding of the Polyporales laccase multigenicity and allows for the systemization of the individual features of different laccase isozymes. In addition, we provide a comparison of the biochemical and catalytic properties of the four laccase isozymes from Trametes hirsuta and suggest their functional diversification within the multigene family.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

et al. 2019

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“…These transcripts are good candidates to investigate whether their encoded LMEs have preferential activities towards S-related aromatics. Previously, syringic acid increased the expression of an mnp in the white-rot fungus Ceriporiopsis subvermispora (Manubens et al, 2003) and of multiple laccases in the white-rot fungus Trametes hirsuta (Moiseenko et al, 2018). The lack of LMEs or H2O2 supply related transcripts with higher expression levels on G-related compared to S-related aromatics may be explained by the presence of G-related ring structures in both cultures, e.g.…”

Section: Differentially Expressed Transcripts On G-compared To S-relamentioning

confidence: 97%

“…As well as aromatics (Manubens et al, 2003;Moiseenko et al, 2018), other factors including metal ions, can affect ligninolytic gene expression and enzyme production. E.g.…”

Section: Introductionmentioning

confidence: 99%

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Daly

Peng

López

et al. 2020

Journal of Biotechnology

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Heterologous production of fungal ligninolytic cocktails is challenging due to the low yields of catalytically active lignin modifying peroxidases. Production using a natural system, such as a wood-rotting fungus, is a promising alternative if specific or preferential induction of the ligninolytic activities could be achieved. Using transcriptomics, gene expression of the white-rot Dichomitus squalens during growth on mixtures of aromatic compounds, with ring structures representing the two major lignin sub-units, was compared to a wood substrate. Most of the genes encoding lignin modifying enzymes (laccases and peroxidases) categorised as highly or moderately expressed on wood were expressed similarly on aromatic compounds. Higher expression levels of a subset of manganese and versatile peroxidases was observed on di-compared to mono-methoxylated aromatics. The expression of polysaccharide degrading enzymes was lower on aromatic compounds compared to wood, demonstrating that the induction of lignin modifying enzymes became more specific. This study suggests potential for aromatic waste streams, e.g. from lignocellulose pretreatment, to produce a lignin-specific enzyme cocktail from D. squalens or other white-rot fungi.

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“…Xylaria spp. are considered to be significant producers of different ligninolytic enzymes, including laccase, xylanase and cellulase [16], besides their ability to degrade lignin [17], hemicellulose [18], and cellulose from trees and agricultural residues [19][20][21][22]. Within the ecosystems, this type of fungus participates in the carbon and nitrogen cycles [23] and plays an important role in the biodegradation processes of wood and leaf litter due to its complex and diverse enzymatic system.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Residues from the Palo Santo (Bursera graveolens) Essential Oil Extraction and Their Potential for Enzyme Production Using Native Xylaria Fungi from Southern Ecuador

et al. 2019

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The degradation dynamics of lignin and cellulose were analyzed by means of a solid state biodegradation experiment, using residues from the essential oil extraction of the Palo Santo tree (Bursera graveolens). As such, two native Xylaria spp. and an exotic mushroom Trametes versicolor were incubated on the spent substrate (Residues of B. Graveolens, BGR’s). The relatively high lignin and cellulose contents of the BGRs (9.1% and 19%, respectively) indicated the potential of this resource for the production of methane (biogas) and ethanol. However, the degradation of the lignin and cellulose content could be traced back to the relatively high activity of the enzymes laccase, cellulase, and xylanase, produced by the fungi. The results showed that laccase (30.0 U/L and 26.6 U/L), cellulase (27.3 U/L and 35.8 U/L) and xylanase (189.7U/L and 128.3 U/L) activities of Xylaria feejeensis and Xylaria cf. microceras were generally higher than T. versicolor (9.0 U/L, 29.5 U/L, 99.5 U/L respectively). Furthermore, the total carbon (TC: 47.3%), total nitrogen (TN: 1.5%), total phosphorus (TP: 0.2%) and total potassium (TK: 1.2%) dynamics were analyzed during the experiment and their importance for the degradation process highlighted. The results of this work might serve as guidance for future studies in dry forest areas, while furthering the understanding of the potential use of native fungi as ecologic lignocellulosic decomposers and for industrial proposes.

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Orchestration of the expression of the laccase multigene family in white-rot basidiomycete Trametes hirsuta 072: Evidences of transcription level subfunctionalization

Cited by 20 publications

References 55 publications

Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

Evolutionary Relationships Between the Laccase Genes of Polyporales: Orthology-Based Classification of Laccase Isozymes and Functional Insight From Trametes hirsuta

Mixtures of aromatic compounds induce ligninolytic gene expression in the wood-rotting fungus Dichomitus squalens

Biodegradation of Residues from the Palo Santo (Bursera graveolens) Essential Oil Extraction and Their Potential for Enzyme Production Using Native Xylaria Fungi from Southern Ecuador

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