Orchestrating vesicle transport, ESCRTs and kinase surveillance during abscission

Chen, Chun‐Ting; Hehnly, Heidi; Doxsey, Stephen

doi:10.1038/nrm3395

Cited by 39 publications

(44 citation statements)

References 61 publications

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“…Nevertheless, the question remains of how the OMM undergoes controlled fission and/or recombination. Again, a comparison with cytokinesis might be instructive, and we now know that this process is tightly regulated by the ESCRT complex (Chen et al, 2012). Although we raise this possibility, there is no current evidence for this mechanism.…”

Section: Nodal Arrangementmentioning

confidence: 85%

Novel roles for actin in mitochondrial fission

Hatch

Gurel

Higgs

2014

Journal of Cell Science

128

130

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Mitochondrial dynamics, including fusion, fission and translocation, are crucial to cellular homeostasis, with roles in cellular polarity, stress response and apoptosis. Mitochondrial fission has received particular attention, owing to links with several neurodegenerative diseases. A central player in fission is the cytoplasmic dynaminrelated GTPase Drp1, which oligomerizes at the fission site and hydrolyzes GTP to drive membrane ingression. Drp1 recruitment to the outer mitochondrial membrane (OMM) is a key regulatory event, which appears to require a pre-constriction step in which the endoplasmic reticulum (ER) and mitochondrion interact extensively, a process termed ERMD (ER-associated mitochondrial division). It is unclear how ER-mitochondrial contact generates the force required for pre-constriction or why pre-constriction leads to Drp1 recruitment. Recent results, however, show that ERMD might be an actin-based process in mammals that requires the ER-associated formin INF2 upstream of Drp1, and that myosin II and other actinbinding proteins might be involved. In this Commentary, we present a mechanistic model for mitochondrial fission in which actin and myosin contribute in two ways; firstly, by supplying the force for preconstriction and secondly, by serving as a coincidence detector for Drp1 binding. In addition, we discuss the possibility that multiple fission mechanisms exist in mammals.

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Section: Nodal Arrangementmentioning

confidence: 85%

Novel roles for actin in mitochondrial fission

Hatch

Gurel

Higgs

2014

Journal of Cell Science

128

130

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“…Recent studies identified the existence of an abscission checkpoint (NoCut) which includes a series of events largely mediated by the Aurora B kinase. 39,40,[44][45][46][47] The most recently identified protein, ANCHR is a novel abscission checkpoint NoCut factor. 38 Interestingly, cellular responses to the manipulations of JADE1S expression levels were reminiscent of that reported for the ANCHR protein.…”

Section: Discussionmentioning

confidence: 99%

The novel function of JADE1S in cytokinesis of epithelial cells

2015

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JADE1 belongs to a small family of PHD zinc finger proteins that interacts with histone acetyl transferase (HAT) HBO1 and is associated with chromatin. We recently reported JADE1 chromatin shuttling and phosphorylation during G2/M to G1 transition, which was sensitive to Aurora A inhibition. In the current study we examined mechanisms of the cell cycle regulation by the small isoform of JADE1 protein, JADE1S, and report data showing that JADE1S has a novel function in the regulation of cytokinesis. Using FACS assays, we show that, JADE1S depletion facilitated rates of G1-cells accumulation in synchronously dividing HeLa cell cultures. Depletion of JADE1S protein in asynchronously dividing cells decreased the proportion of cytokinetic cells, and increased the proportion of multi-nuclear cells, indicative of premature and failed cytokinesis. In contrast, moderate overexpression of JADE1S increased the number of cytokinetic cells in time-and dose-dependent manner, indicating cytokinetic delay. Pharmacological inhibition of Aurora B kinase resulted in the release of JADE1S-mediated cytokinetic delay and allowed progression of abscission in cells overexpressing JADE1S. Finally, we show that JADE1S protein localized to centrosomes in interphase and mitotic cells, while during cytokinesis JADE1S localized to the midbody. Neither JADE1L nor partner of JADE1, HAT HBO1 was localized to the centrosomes or midbodies. Our study identifies the novel role for JADE1S in regulation of cytokinesis and suggests function in Aurora B kinase-mediated cytokinesis checkpoint.

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“…Of note, centriolin shares a small region of homology with the fission yeast SIN scaffolding component Cdc11 [122]. How centriolin, Cep55 and other centrosome-and midbody-associated proteins cooperate to regulate mitotic exit in human cells remains to be elucidated, but they are expected to impact on the regulation of vesicle transport and MT severing [124,125].…”

Section: Centrosomes As Signalling Platforms In Vertebrates? (A) Cellmentioning

confidence: 99%

Centrosomes as signalling centres

Arquint

Gabryjonczyk

Nigg

2014

Phil. Trans. R. Soc. B

131

114

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Centrosomes—as well as the related spindle pole bodies (SPBs) of yeast—have been extensively studied from the perspective of their microtubule-organizing roles. Moreover, the biogenesis and duplication of these organelles have been the subject of much attention, and the importance of centrosomes and the centriole–ciliary apparatus for human disease is well recognized. Much less developed is our understanding of another facet of centrosomes and SPBs, namely their possible role as signalling centres. Yet, many signalling components, including kinases and phosphatases, have been associated with centrosomes and spindle poles, giving rise to the hypothesis that these organelles might serve as hubs for the integration and coordination of signalling pathways. In this review, we discuss a number of selected studies that bear on this notion. We cover different processes (cell cycle control, development, DNA damage response) and organisms (yeast, invertebrates and vertebrates), but have made no attempt to be comprehensive. This field is still young and although the concept of centrosomes and SPBs as signalling centres is attractive, it remains primarily a concept—in need of further scrutiny. We hope that this review will stimulate thought and experimentation.

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Orchestrating vesicle transport, ESCRTs and kinase surveillance during abscission

Cited by 39 publications

References 61 publications

Novel roles for actin in mitochondrial fission

Novel roles for actin in mitochondrial fission

The novel function of JADE1S in cytokinesis of epithelial cells

Centrosomes as signalling centres

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