Orally administered nicotine effects on rat urinary bladder proliferation and carcinogenesis

Suzuki, Shugo; Cohen, Samuel M.; Arnold, Lora L.; Kato, Hiroyuki; Fuji, Satoshi; Pennington, Karen L.; Nagayasu, Yuko; Naiki‐Ito, Aya; Yamashita, Yoshihisa; Takahashi, Satoru

doi:10.1016/j.tox.2018.02.008

Cited by 17 publications

(28 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanosystems that increase lung persistence of antibiotic drugs are of great interest and much needed, 23 particularly for those patients who suffer from recurrent pulmonary infections and require high local concentrations and low systemic distribution of the drug. Polymeric particles are used to overcome solubility issues that impair absorption and can cause drug aggregation and associated toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…To determine in vitro cytotoxicity of M33-NS, compared to the free SET-M33 peptide and the nanocarrier, immortalized 16HBE14o-human bronchial epithelial cells 22 and T24 human urinary bladder epithelial carcinoma cells 23 were used as model systems. The bronchial epithelium is clearly the tissue most exposed to aerosol administration, whereas in case of absorption and subsequent systemic availability, urinary clearance also has to be kept in mind, as urinary clearance was observed in a previous study.…”

Section: Cytotoxicity In Lung Epithelial Cells Bladder Cells and Macmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for Pseudomonas aeruginosa Infections

Falciani

Zevolini

Brunetti

et al. 2020

IJN

View full text Add to dashboard Cite

Introduction: Antibiotic-resistant bacteria kill 25,000 people every year in the EU. Patients subject to recurrent lung infections are the most vulnerable to severe or even lethal infections. For these patients, pulmonary delivery of antibiotics would be advantageous, since inhalation can achieve higher concentration in the lungs than iv administration and can provide a faster onset of action. This would allow for the delivery of higher doses and hence reduce the number of treatments required. We report here about a new nanosystem (M33-NS) obtained by capturing SET-M33 peptide on single-chain dextran nanoparticles. SET-M33 is a non-natural antimicrobial peptide synthesized in branched form. This form gives the peptide resistance to degradation in biological fluids. SET-M33 has previously shown efficacy in vitro against about one hundred of Gram-negative multidrug and extensively drug-resistant clinical isolates and was also active in preclinical infection models of pneumonia, sepsis and skin infections. Methods: The new nanosystem was evaluated for its efficacy in bacteria cells and in a mouse model of pneumonia. Toxicity and genotoxicity were also tested in vitro. Biodistribution and pharmacokinetic studies in healthy rats were carried out using a radiolabeled derivative of the nanosystem. Results: The M33-nanosystem, studied here, showed to be effective against Pseudomonas aeruginosa in time-kill kinetic experiments. Cytotoxicity towards different animal cell lines was acceptable. Lung residence time of the antimicrobial peptide, administered via aerosol in healthy rats, was markedly improved by capturing SET-M33 on dextran nanoparticles. M33-NS was also efficient in eradicating pulmonary infection in a BALB/c mouse model of pneumonia caused by P. aeruginosa. Discussion: This study revealed that the encapsulation of the antimicrobial peptide in dextran nanoparticles markedly improved lung residence time of the peptide administered via aerosol. The result has to be considered among the aims of the development of a new therapeutic option for patients suffering recurrent infections, that will benefit from high local doses of persistent antimicrobials.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Cytotoxicity In Lung Epithelial Cells Bladder Cells and Macmentioning

confidence: 99%

Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for Pseudomonas aeruginosa Infections

Falciani

Zevolini

Brunetti

et al. 2020

IJN

View full text Add to dashboard Cite

show abstract

“…Interestingly, smoker-men were reported to have UBC more frequently than smoker-women, and accordingly, cigarette-smoking and male gender have been regarded as co-risk factors for UBC [16]. Emerging data suggest that nicotine exposure may enhance tumor growth and metastasis, and there is a growing body of evidence depicting that nicotine promotes cell proliferation, angiogenesis, and epithelial-to-mesenchymal transition through nicotinic acetylcholine receptors found in the urinary bladder, leading to enhanced tumor growth and metastasis [17]. Assessment of TLR7 serum level in UBC patients revealed that such serum marker was downregulated.…”

Section: Discussionmentioning

confidence: 99%

Serum level and single-nucleotide polymorphisms of toll-like receptor-7 among urinary bladder cancer Iraqi patients

Al-Humairi

Al-Musawi

Ad’hiah

2019

Egypt J Med Hum Genet

View full text Add to dashboard Cite

Background: Toll-like receptor 7 (TLR7), a member of TLR family, plays a pivotal role in pathogenesis of different malignancies. Among these is urinary bladder cancer (UBC), which has not been extensively studied. Therefore, it was aimed to determine TLR7 serum level in UBC patients and evaluate its association with some demographic and clinicopathological characteristics. In addition, four TLR7 single-nucleotide polymorphisms (SNPs: rs179018, rs179019, rs179020, and rs179021) were investigated to determine their susceptibility role in UBC and inspect SNP's impact on TLR7 level. Sixty-six UBC Iraqi patients were enrolled in this case-control study. Two control samples were also involved, 40 urinary tract infection (UTI) patients, and 48 healthy control subjects. Results: Male gender, older age, and cigarette-smoking are risk factors for UBC. TLR7 level showed a significant decreased median in UBC patients compared to UTI patients or control (1.4 vs. 8.1 and 9.5 ng/ml, respectively; p < 0.001). The decrease was more pronounced in males, age group ≥ 48 years, cigarette-smokers, alcohol non-consumers, clinical stages I-II, and superficial tumor, as well as patients with family history of cancer and untreated patients. Mitomycin C and Bacillus Calmette-Guérin therapies tended to increase TLR7 level. Among the four investigated SNPs, only rs179019 C allele showed significantly uncorrected increased frequency in UBC males compared to control males (p = 0.038), while among UTI females, C allele frequency maintained a significantly corrected decreased frequency compared to control females (p = 0.005). Some SNPs influenced serum level of TLR7, but a significant impact was recorded for rs179019 in UTI females (p = 0.006). Conclusions: Downregulation of TLR7 is suggested to have a role in etiology and pathogenesis of UBC, especially the male, elderly and smoker patients. Mitomycin C and Bacillus Calmette-Guérin may enhance TLR7 production in the blood of UBC patients. TLR7 SNPs are suggested to influence susceptibility to develop UBC, and their potential in impacting TLR7 serum level is augmented.

show abstract

“…Cell death in response to CS in the larynx has not been well elucidated. Immunohistochemical evaluation is the primary means to detect CS-induced epithelial cell death [ 48 , 51 , 52 ]. However, these evaluations may not provide complete insight into CS-induced cytotoxic responses.…”

Section: Introductionmentioning

confidence: 99%

Short-term whole body cigarette smoke exposure induces regional differences in cellular response in the mouse larynx

et al. 2021

View full text Add to dashboard Cite

show abstract

Orally administered nicotine effects on rat urinary bladder proliferation and carcinogenesis

Cited by 17 publications

References 26 publications

<p>Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for <em>Pseudomonas aeruginosa</em> Infections</p>

<p>Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for <em>Pseudomonas aeruginosa</em> Infections</p>

Serum level and single-nucleotide polymorphisms of toll-like receptor-7 among urinary bladder cancer Iraqi patients

Short-term whole body cigarette smoke exposure induces regional differences in cellular response in the mouse larynx

Contact Info

Product

Resources

About