Orally administered gadolinium orthovanadate GdVO4:Eu3+ nanoparticles do not affect the hydrophobic region of cell membranes of leukocytes

Tkachenko, Anton; Klochkov, Vladimir K.; Lesovoy, Vladimir; Myasoedov, Valerij; Kavok, Nataliya; Onishchenko, Anatolii; Yefimova, Svetlana; Posokhov, Yevgen O.

doi:10.1007/s10354-020-00735-4

Cited by 7 publications

(1 citation statement)

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“…Inorganic NPs have been demonstrated to possess several mechanisms for intracellular toxicity: disruption of cell membranes, reactive oxygen species (ROS) generation, induction of pro-inflammatory cytokines, corrosion of metal-containing NPs in the acidic medium of lysosomes (the so-called "lysosomeenhanced Trojan horse effect hypothesis"), induction of cell death such as apoptosis and autophagy, and others [16][17][18][19]. VNPs at the dose used in this study are non-toxic [20]. Thus, taking into account their antioxidant properties and relative safety, VNPs can be assessed for their possible application as antiinflammatory agents.…”

Section: Introductionmentioning

confidence: 99%

Gadolinium Orthovanadate GdVO4:Eu3+ Nanoparticles Ameliorate Carrageenan-Induced Intestinal Inflammation

Tkachenko¹,

Pogozhykh²,

Onishchenko³

et al. 2021

J. Pharm. Nutr. Sci.

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Gadolinium orthovanadate GdVO4:Eu3+ nanoparticles (VNPs) have been shown to scavenge reactive oxygen species (ROS), making them a promising therapeutic agent in inflammation.This study aims to assess the effects of VNPs administered orally on E407a-induced inflammation.Materials and Methods: Fragments of the small intestine of 8 rats treated orally with a carrageenan-containing food additive E407a at a dose of 140 mg / kg of weight during 2 weeks, 8 animals orally exposed to both E407a and VNPs at a dose of 20 µg / kg of weight during the same period of time, and 8 control rats were stained routinely and immunostained for CD3 and CD68 with the subsequent immunohistochemical scoring. Moreover, analysis of viability and cell death modes of granulocytes was performed by flow cytometry using Annexin V and 7aminoactinomycin D (7-AAD).Results: Oral exposure to the food additive E407a resulted in the development of enteritis associated with altered small intestinal morphology, infiltration of the lamina propria with macrophages and T-lymphocytes, and activation of peripheral blood granulocyte apoptosis. VNPs administered against the background of E407a-induced slight intestinal inflammation improved small intestinal morphology, decreased infiltration rate of the immune cells mentioned above without affecting the intensity of granulocyte apoptosis. Conclusion:Oral administration of VNPs ameliorates E407a-induced enteritis.

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