Oral vaccination of mice with lipid‐encapsulated <i>Mycobacterium bovis</i> BCG: Anatomical sites of bacterial replication and immune activity

Aldwell, Frank E.; Baird, Margaret A.; Fitzpatrick, Clare; McLellan, Alexander D.; Cross, Martin L.; Lambeth, Matthew R.; Buchan, Glenn

doi:10.1111/j.1440-1711.2005.01369.x

Cited by 35 publications

(35 citation statements)

References 21 publications

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“…While many novel TB vaccines have been developed [4,5], few have demonstrated protective efficacy that can surpass BCG, which remains the only vaccine approved for human use. Previous research indicates that addition of adjuvant components to the BCG vaccine significantly enhances immune responses to promote host protection against subsequent challenge with virulent MTB [6][7][8][9]. One such reported component is lactoferrin, an iron binding protein found primarily in mucosal secretions and secondary granules of neutrophils [10].…”

Section: Introductionmentioning

confidence: 99%

Influence of bovine lactoferrin on expression of presentation molecules on BCG-infected bone marrow derived macrophages

Hwang¹,

Kruzel

Actor³

2009

Biochimie

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The current vaccine for tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB), is an attenuated strain of Mycobacterium bovis bacillus Calmette-Guerin (BCG). BCG has proven to be effective in children, however, efficacy wanes in adulthood. Lactoferrin, a natural protein with immunomodulatory properties, is a potential adjuvant candidate to enhance efficacy of BCG. These studies define bovine lactoferrin as an enhancer of the BCG vaccine, functioning in part by modulating macrophage ability to present antigen and stimulate T-cells. BCG-infected bone marrow derived macrophages (BMMs) cultured with bovine lactoferrin increased the number of MHC II + expressing cells. Addition of IFN-γ and lactoferrin to BCG-infected BMMs enhanced MHC II expressiona dna increased the ratio of CD86/CD80. Lactoferrin treated BCG-infected BMMs were able to stimulate an increase in IFN-γ production from presensitized CD3 + splenocytes. Together, these results demonstrate that bovine lactoferrin is capable of modulating BCG-infected macrophages to enhance T-cell stimulation through increased surface expression of antigen presentation and costimulatory molecules, which potentially explains the observed in vivo bovine lactoferrin enhancement of BCG vaccine efficacy to protect against virulent MTB infection.

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Section: Introductionmentioning

confidence: 99%

Influence of bovine lactoferrin on expression of presentation molecules on BCG-infected bone marrow derived macrophages

Hwang¹,

Kruzel

Actor³

2009

Biochimie

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“…Since BCG is a live-attenuated bacterium, it needs to be protected from degradation in the stomach of an animal if it is to be deployed as an oral vaccine. An edible lipid matrix has been developed that allows BCG bacilli to be maintained in a viable but static state that is suitable as an oral delivery vehicle for the vaccine (Aldwell et al 2005). Studies in a range of animal species have shown that oral vaccination with this lipid-formulated BCG can induce a level of protection against experimental challenge with Tb that is comparable with that induced by injecting the vaccine (Aldwell et al 2003a,b;Buddle et al 2006;Nol et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Oral vaccination reduces the incidence of tuberculosis in free-living brushtail possums

et al. 2009

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Bovine tuberculosis (Tb) caused by Mycobacterium bovis has proved refractory to eradication from domestic livestock in countries with wildlife disease reservoirs. Vaccination of wild hosts offers a way of controlling Tb in livestock without wildlife culling. This study was conducted in a Tb-endemic region of New Zealand, where the introduced Australian brushtail possum (Trichosurus vulpecula) is the main wildlife reservoir of Tb. Possums were trapped and vaccinated using a prototype oraldelivery system to deliver the Tb vaccine bacille Calmette -Guerin. Vaccinated and control possums were matched according to age, sex and location, re-trapped bimonthly and assessed for Tb status by palpation and lesion aspiration; the site was depopulated after 2 years and post-mortem examinations were conducted to further identify clinical Tb cases and subclinical infection. Significantly fewer culture-confirmed Tb cases were recorded in vaccinated possums (1/51) compared with control animals (12/71); the transition probability from susceptible to infected was significantly reduced in both males and females by vaccination. Vaccine efficacy was estimated at 95 per cent (87-100%) for females and 96 per cent (82-99%) for males. Hence, this trial demonstrates that orally delivered live bacterial vaccines can significantly protect wildlife against natural disease exposure, indicating that wildlife vaccination, along with existing control methods, could be used to eradicate Tb from domestic animals.

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“…One approach may involve targeting the intestinal lymphatic regions, which have been routinely explored and used for site-specific lymphatic delivery of orally administered proteins, drugs, and vaccines (4,83,271). Some alternative Se-delivery approaches have been attempted and shown to ameliorate colitis in an animal model (168).…”

Section: Can Se Supplementation Be Targeted To the Immune System?mentioning

confidence: 99%

The Role of Selenium in Inflammation and Immunity: From Molecular Mechanisms to Therapeutic Opportunities

Huang

Rose

Hoffmann

2012

Antioxidants & Redox Signaling

658

496

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Dietary selenium (]Se), mainly through its incorporation into selenoproteins, plays an important role in inflammation and immunity. Adequate levels of Se are important for initiating immunity, but they are also involved in regulating excessive immune responses and chronic inflammation. Evidence has emerged regarding roles for individual selenoproteins in regulating inflammation and immunity, and this has provided important insight into mechanisms by which Se influences these processes. Se deficiency has long been recognized to negatively impact immune cells during activation, differentiation, and proliferation. This is related to increased oxidative stress, but additional functions such as protein folding and calcium flux may also be impaired in immune cells under Se deficient conditions. Supplementing diets with above-adequate levels of Se can also impinge on immune cell function, with some types of inflammation and immunity particularly affected and sexually dimorphic effects of Se levels in some cases. In this comprehensivearticle, the roles of Se and individual selenoproteins in regulating immune cell signaling and function are discussed. Particular emphasis is given to how Se and selenoproteins are linked to redox signaling, oxidative burst, calcium flux, and the subsequent effector functions of immune cells. Data obtained from cell culture and animal models are reviewed and compared with those involving human physiology and pathophysiology, including the effects of Se levels on inflammatory or immune-related diseases including anti-viral immunity, autoimmunity, sepsis, allergic asthma, and chronic inflammatory disorders. Finally, the benefits and potential adverse effects of intervention with Se supplementation for various inflammatory or immune disorders are discussed. Antioxid. Redox Signal. 16, 705-743.

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Oral vaccination of mice with lipid‐encapsulated Mycobacterium bovis BCG: Anatomical sites of bacterial replication and immune activity

Cited by 35 publications

References 21 publications

Influence of bovine lactoferrin on expression of presentation molecules on BCG-infected bone marrow derived macrophages

Influence of bovine lactoferrin on expression of presentation molecules on BCG-infected bone marrow derived macrophages

Oral vaccination reduces the incidence of tuberculosis in free-living brushtail possums

The Role of Selenium in Inflammation and Immunity: From Molecular Mechanisms to Therapeutic Opportunities

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