Oral tolerance induction to Dermatophagoides pteronyssinus and Blomia tropicalis in sensitized mice: occurrence of natural autoantibodies to immunoglobulin E

Abstract

This is the first time that the murine-type I hypersensitivity is employed to study Bt-immunization, showing a marked IgE production, associated with IgG response, which is at least in part driven by T-independent antigens. The oral tolerance protocol in previously sensitized animals was able to down-modulate IgE response and points out this route as a strategy for allergy therapy.

Oral administration of allergen extracts from Dermatophagoides farinae desensitizes specific allergen-induced inflammation and airway hyperresponsiveness in rats

“…The conventionally used allergens include house mite allergen [19][20][21][22], pollen extract [23,24], ovalbumin [25] and peanut protein [26], Many studies showed that oral administration of allergen, including recombinant Derf2, effectively suppresses IgE responses in the allergic model of mice [19,20]. Although we supposed that the effects of Derfdrop is antigen-specific and it is not likely that Derfdrop affects immune response via antigen-non-specific way, it is still necessary to set up the experiments to verify the specificity of Derf allergen by using other allergen-induced asthmatic animal model.…”

Intranasal sensitization with Blomia Tropicalis antigens induces allergic responses in mice characterized by elevated antigen-specific and non-specific serum ige and peripheral blood eosinophil counts

“…In their study to induce oral tolerance of IgE response to B. tropicalis, SATO et al demonstrated that IgE levels were markedly increased in mice by subcutaneous immunization of Bt antigen 24 . In the present study, the authors have first demonstrated that B. tropicalis-derived antigens could systematically induce allergic responses in mice by nasal route, providing the direct evidence that the mite antigens could function as potential allergens in vivo if administered properly.…”

“…These facts strongly suggest that B. tropicalis contains allergen(s) with the potential to induce systemic IgE responses in humans 4,5 . However, there were few experimental studies to demonstrate the Bt-specific IgE production by antigen exposure 24 . In the present study, the authors tried to demonstrate Bt antigen-specific serum IgE production in mice exposed to Bt antigen by nasal route.…”

Long-term anergy in orally tolerized mice is linked to decreased B7.2 expression on B cells

“…An enzyme-linked immunosorbent assay (ELISA) was performed to detect IgG isotypes as previously described (Sato et al, 2002). Briefly, 96-well microplates (Costar, Cambridge, MA) were coated with 5 mg/ml of OVA in 0.1 M carbonate-bicarbonate buffer, pH ¼ 9.5.…”