Oral Tolerance Induction in<i>Dermatophagoides pteronyssinus</i>-Sensitized Mice Induces Inhibition of IgE Response and Upregulation of TGF-<i>β</i>Secretion

Sato, Maria Notomi; Fusaro, Ana Elisa; Victor, Jefferson Russo; Oliveira, C. R.; Futata, E. T.; Maciel, Milton; Carvalho, Alex Franco de; Duarte, Alberto José da Silva

doi:10.1089/107999001753238079

Cited by 14 publications

(13 citation statements)

References 34 publications

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“…Our previous data demonstrated increased TGF-ß basal secretion levels by mesenteric lymph nodes (11), as well as by spleen cells in mice fed with the Dp extract (16). In the present study, a decreased IFN-γ production by peripheral lymph node cells was observed four days after Dp extract feeding.…”

Section: Evidence For Serum and Secretory Polyreactive Iga Antibodiessupporting

confidence: 72%

“…to the dust mite Dp extract in mice showed that oral administration down-modulated the Th2-axis, including IgE (11,15), IgG1 and IL-4 production and up-regulated TGF-ß secretion (16). However, the complex regulatory cytokine network triggered by oral tolerance, regarding the specific and natural polyreactive IgA response in the serum and gut secretion of allergen-tolerized mice is not completely understood.…”

Section: Evidence For Serum and Secretory Polyreactive Iga Antibodiesmentioning

confidence: 99%

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IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

Maciel

Fusaro

Oliveira

et al. 2004

Braz J Med Biol Res

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Induced oral tolerance to mucosal-exposed antigens in immunized animals is of particular interest for the development of immunotherapeutic approaches to human allergic diseases. This is a unique feature of mucosal surfaces which represent the main contact interface with the external environment. However, the influence of oral tolerance on specific and natural polyreactive IgA antibodies, the major defense mechanism of the mucosa, is unknown. We have shown that oral administration of an extract of the dust mite Dermatophagoides pteronyssinus (Dp) to primed mice caused down-regulation of IgE responses and an increase in tumor growth factor-ß secretion. In the present study, we observed that primed inbred female A/Sn mice (8 to 10 weeks old) fed by gavage a total weight of 1.0-mg Dp extract on the 6th, 7th and 8th days post-immunization presented normal secretion of IL-4 and IL-10 in gut-associated lymphoid tissue and a decreased production of interferon gamma induced by Dp in the draining lymph nodes (13,340 ± 3,519 vs 29,280 ± 2,971 pg/ml). Mice fed the Dp extract also showed higher levels of serum anti-Dp IgA antibodies and an increase of IgA-secreting cells in mesenteric lymph nodes (N = 10), reflecting an increase in total fecal IgA antibodies (N = 10). The levels of secretory anti-Dp IgA antibodies increased after re-immunization regardless of Dp extract feeding. Oral tolerance did not interfere with serum or secretory IgA antibody reactivity related to self and non-self antigens. These results suggest that induction of oral tolerance to a Dp extract in sensitized mice triggered different regulatory mechanisms which inhibited the IgE response and stimulated systemic and secretory IgA responses, preserving the natural polyreactive IgA antibody production.

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Section: Evidence For Serum and Secretory Polyreactive Iga Antibodiessupporting

confidence: 72%

Section: Evidence For Serum and Secretory Polyreactive Iga Antibodiesmentioning

confidence: 99%

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

Maciel

Fusaro

Oliveira

et al. 2004

Braz J Med Biol Res

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“…Oral alloantigen administered continuously just after grafting was more effective than when administered one week after the graft. In another murine model of oral tolerance induction such as type I hypersensitivity to dust mite (14,15), a dose-dependent effect on the modulation of the IgE response was observed in sensitized mice. An additional increase in the allergen dose enhanced the hypersensitivity response, worsening the allergic response (14,15).…”

Section: Discussionmentioning

confidence: 98%

“…In another murine model of oral tolerance induction such as type I hypersensitivity to dust mite (14,15), a dose-dependent effect on the modulation of the IgE response was observed in sensitized mice. An additional increase in the allergen dose enhanced the hypersensitivity response, worsening the allergic response (14,15). Similar to the effect obtained with antigen delivery to the mucosal site, injection of donor splenocytes into the portal vein prolonged skin allograft survival.…”

Section: Discussionmentioning

confidence: 98%

Induction of oral tolerance and the effect of interleukin-4 on murine skin allograft rejection

Dettino

Duarte

Sato

2004

Braz J Med Biol Res

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“…TGFβ1 participates in the local regulation of allergen-specific IgE production and peripheral tolerance to allergens [52]. It has been reported that addition of recombinant TGFβ1 to spleen cell cultures stimulated with D. pteronyssinus inhibits IgE secretion [53]. Also oral administration of high-dose TGFβ1 simultaneously with ovalbumin significantly inhibits the elevation of ovalbumin-specific IgE levels and anaphylactic reactions in mice [54].…”

Section: Discussionmentioning

confidence: 99%

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

Acevedo

Vergara

Gusmão

et al. 2009

Int Arch Allergy Immunol

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Background: The C-509T polymorphism of the transforming growth factor beta-1 (TGFB1) gene has been associated with asthma and asthma-related phenotypes, but its influence on total and specific IgE levels is controversial. Objective: To investigate the association between C-509T and asthma, as well as total IgE and specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus, in a Colombian population. Methods: The study population consisted of 417 asthmatics, 390 controls and 116 nuclear families. The C-509T polymorphism was genotyped using single-base extension minisequencing or Taq Man probes. IgE and TGFβ1 levels were measured by ELISA. Regression analysis and family-based association tests were performed in cases/controls and families. Associations were corrected by population structure using a panel of 52 ancestry informative markers in the case-control dataset. Results: There was no association between C-509T and asthma. In asthmatics, the CC genotype was associated with higher total IgE levels compared with the other genotypes [mean IgE: 2.81 ± 0.42 vs. 2.71 ± 0.45 log IU/ml; p = 0.016, corrected p value (p_c) = 0.019]. When only atopic asthmatics were included, the significance remained (p = 0.02, p_c = 0.03). In the family-based analyses, the C allele was associated with higher total IgE levels (p = 0.02) and the CC genotype with specific IgE to D. pteronyssinus (p = 0.01). Conclusions: C-509T is associated with total IgE levels and specific IgE to D. pteronyssinus in asthmatic patients. In contrast to other studies, we found the CC genotype to be associated with higher levels of total and specific IgE. Differences in the frequency of this allele among populations could alter its effects as a risk factor for asthma-associated phenotypes.

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Oral Tolerance Induction inDermatophagoides pteronyssinus-Sensitized Mice Induces Inhibition of IgE Response and Upregulation of TGF-βSecretion

Cited by 14 publications

References 34 publications

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

IgA response in serum and gut secretion in sensitized mice fed with the dust mite Dermatophagoides pteronyssinus extract

Induction of oral tolerance and the effect of interleukin-4 on murine skin allograft rejection

The C-509T Promoter Polymorphism of the Transforming Growth Factor Beta-1 Gene Is Associated with Levels of Total and Specific IgE in a Colombian Population

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