Oral submucous fibrosis: Review on aetiology and pathogenesis

Tilakaratne, Wanninayake Mudiyanselage; Klinikowski, M.F.; Saku, Takashi; Peters, Timothy J.; Warnakulasuriya, Saman

doi:10.1016/j.oraloncology.2005.08.005

Cited by 549 publications

(468 citation statements)

References 53 publications

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“…Over the past two decades, numerous studies have been conducted on the roles of the constituents of areca nut in the pathogenesis of OSMF. OSMF can be considered as a collagen metabolic disorder [23]. TGF-β plays a major role in wound repair, fibrosis and in the development of many fibrous diseases [24].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Myofibroblasts By Expression of Alpha Smooth Muscle Actin: A Marker in Fibrosis, Dysplasia and Carcinoma

Rao

Narasimhan

Narashiman

et al. 2014

JCDR

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Section: Resultsmentioning

confidence: 99%

Evaluation of Myofibroblasts By Expression of Alpha Smooth Muscle Actin: A Marker in Fibrosis, Dysplasia and Carcinoma

Rao

Narasimhan

Narashiman

et al. 2014

JCDR

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“…As OSF shows a gross imbalance in ECM remodeling, this putative mechanism was investigated in vitro [29]. A decrease of the phagocytic activity of fibroblasts in OSF patients when compared with the normal persons has been earlier reported [31].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Interventions in Oral Submucous Fibrosis: An Experimental and Clinical Study

Singh

Mohammad

Pant³

et al. 2014

J. Maxillofac. Oral Surg.

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Introduction Oral submucous fibrosis (OSF) is a chronic debilitating disease and premalignant condition of the oral cavity and is a serious public health issue in India and many parts of the world. The treatment is still elusive and empirical because of poorly understood etiopathogenesis, which is believed to be multifactorial including areca nut chewing, ingestion of chillies, genetic and immunologic processes, nutritional deficiencies, and many others. The present investigations was focused to understand the possible therapeutic interventions of anti-OSF agents in arecoline induced experimental in vitro model of OSF and clinical application of these anti-OSF agents in the restoration of various grade of the disease. Materials and Methods The 127 subjects were selected from patients who visited the OPD of Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, K.G. Medical University, Lucknow. Further the subjects were divided in two groups on the basis of clinical examination. Group-1 subjects showed presence of fibrosis bands in the labial and/or buccal mucosa, loss of elasticity, difficulty to open the mouth and had a habit chewing arecanut in some form. Group-2 subjects had no habit of chewing areca-nut, were apparently healthy with no mucosal disorder. The samples were collected and were immediately transported to Indian Institute of Toxicology Research, Lucknow, for isolation and cultivation of primary cultures of mucosal fibroblast cells. Then isolation and cultivation of oral mucosal fibroblast, identification of non-cytotoxic doses of arecoline, real time PCR, immunocytochemistry, cytokine determination in culture cells, western blot analyses, functional activity of collagenase, lysyl oxidase enzyme activity, collagen beads assay, cyclooxygenase (COX-2) expression analysis was done. Results and Conclusions This study, shows that the reduction of phagocytic cells was strongly related to the arecoline levels in fibroblast culture when we exposed arecoline to normal oral mucosal cells (NOMC) and cells from OSF patient. An enhancement of phagocytic cells was observed following the pre exposure of cells to 1 lM dexamethasone, a glucocorticoids, In this study, histologic evidence is presented which supports the finding that COX-2 expression is upregulated in OSF specimens compared to normal oral submucosal cells. Strong immunostaining for COX-2 was detected in arecoline exposed NOMC and cells from OSF patient. Areca nut extract up-regulates prostaglandin production, cyclooxygenase-2 mRNA and protein expression of human oral keratinocytes. The number of phagocytic cells and phagocytic activity in cultured human oral fibroblasts from OSF site was lower than the fibroblasts from the normal regions of the same person.

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“…In patients with oral submucous fibrosis, the oral epithelium becomes atrophic and thereby becomes more vulnerable to carcinogens [6,7]. Recently, the carcinogenicity of areca nut without tobacco was identified [8].…”

Section: Discussionmentioning

confidence: 99%