Oral probiotics and the female urinary microbiome: a double-blinded randomized placebo-controlled trial

Wolff, Birte; Price, Travis K.; Joyce, Cara; Wolfe, Alan J.

doi:10.1007/s11255-019-02282-3

Cited by 26 publications

(20 citation statements)

References 18 publications

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“…However, upon ineffective strains removal, a statistically significant decrease was found in recurrent UTI patients treated with probiotic lactobacilli ( Grin et al, 2013 ). Wolff et al (2019) conducted a double-blinded RCT to evaluate if oral probiotics could induce changes in the uropathogens/ Lactobacillus (U/L) ratio among healthy premenopausal young women. Oral administration of L. rhamnosus GR-1 and L. reuteri RC-14 induced no considerable changes in U/L ratio in healthy female individuals.…”

Section: Health-boosting Spectrum Of Probioticsmentioning

confidence: 99%

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

2020

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Probiotics are considered as the twenty-first century panpharmacon due to their competent remedial power to cure from gastrointestinal dysbiosis, systematic metabolic diseases, and genetic impairments up to complicated neurodegenerative disorders. They paved the way for an innovative managing of various severe diseases through palatable food products. The probiotics’ role as a “bio-therapy” increased their significance in food and medicine due to many competitive advantages over traditional treatment therapies. Their prophylactic and therapeutic potential has been assessed through hundreds of preclinical and clinical studies. In addition, the food industry employs probiotics as functional and nutraceutical ingredients to enhance the added value of food product in terms of increased health benefits. However, regardless of promising health-boosting effects, the probiotics’ efficacy still needs an in-depth understanding of systematic mechanisms and factors supporting the healthy actions.

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Section: Health-boosting Spectrum Of Probioticsmentioning

confidence: 99%

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

2020

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“…Phases I and III flanked an experimental phase (i.e., Phase II), in which the participants were randomized to take an oral probiotic or placebo. This part of the study has been published 34 . MSU, peri-urethral swabs, and a personal questionnaire were collected daily throughout all three phases.…”

Section: Profum Study Enrollment and Specimen Collectionmentioning

confidence: 99%

Temporal Dynamics of the Adult Female Lower Urinary Tract Microbiota

Price¹,

Wolff

Halverson³

et al. 2020

Preprint

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245/250) 24MANUSCRIPT: (5000/5000) 25 ACKNOWLEDGMENTS: We thank Mary Tulke RN for her assistance with participant 35 recruitment. We also acknowledge funding from NIH (R01 DK104718 awarded to AJW 36and LB). The funders did not play a part in the design or conduct of the study. 37 TKP, BW, LB, ERM, and AJW designed the study. TKP and BW recruited participants. 38TKP processed the specimens. TH prepared them for DNA sequencing. RL sequenced 39 the specimens. TKP, BW, QD and AJW analyzed the data. TKP and AJW wrote the 40 manuscript. TKP, BW, TH, RL, LB, QD, ERM, and AJW reviewed and edited the 41 manuscript. 42 43 ABSTRACT 59Temporal dynamics of certain human microbiotas have been described in 60 longitudinal studies; variability often relates to modifiable factors or behaviors. Early 61 studies of the urinary microbiota preferentially used samples obtained by transurethral 62 catheterization to minimize vulvo-vaginal microbial contributions. Whereas voided 63 specimens are preferred for longitudinal studies, the few studies that reported 64 longitudinal data were limited to women with lower urinary tract (LUT) symptoms, due to 65 ease of accessing a clinical population for sampling and the impracticality and risk of 66 collecting repeated catheterized urine specimens in a non-clinical population. Here, we 67 studied the microbiota of the LUT of non-symptomatic, pre-menopausal women using 68 mid-stream voided urine (MSU) specimens to investigate relationships between 69 microbial dynamics and personal factors. Using 16S rRNA gene sequencing and a 70 metaculturomics method called Expanded Quantitative Urine Culture (EQUC), we 71 characterized the microbiotas of MSU and peri-urethral swab specimens collected daily 72 for approximately three months from a small cohort of adult women. Participants were 73 screened for eligibility, including ability to self-collect paired urogenital specimens prior 74 to enrollment. In this population, we found that measures of microbial dynamics related 75 to specific participant-reported factors, particularly menstruation and vaginal 76intercourse. Further investigation of the trends revealed differences in composition and 77 diversity of LUT microbiotas within and across participants. These data, in combination 78 with previous studies showing relationships between the LUT microbiota and LUT 79 symptoms, suggest that personal factors relating to the genitourinary system may be an 80 important consideration in the etiology, prevention, and/or treatment of LUT disorders.

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“…Wolff et al studied the changes in the ratio between uropathogens and Lactobacillus (U/L) within the lower UTI in response to oral probiotic supplementation. Based on the results, there were no changes between groups in terms of microbiota diversity and the use of oral probiotic did not alter the U/L ratio [113]. The physicochemical cell surface, adhesion properties, and the antagonistic activity of recombinant Lactococcus lactis containing the Ama r 2 gene against the E. coli causing UTI in humans were studied.…”

Section: Probioticsmentioning

confidence: 98%

Deep insights into urinary tract infections and effective natural remedies

et al. 2021

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Background Urinary tract infection (UTI) is a common occurrence in females, during pregnancy, and in peri- and postmenopausal women. UTIs are associated with significant morbidity and mortality, and they affect the quality of life of the affected patients. Antibiotic therapy is an effective approach and reduces the duration of symptoms. Development of resistance, adverse effects of antibiotics, and other associated problems lead to establishing the research framework to find out the alternative approaches in controlling UTIs. Natural approaches have been extensively used for the management of various diseases to improve symptoms and also improve general health. Main body Different databases were employed to identify studies reporting on natural options including herbal medicines, vitamins, trace elementals, sugars, and probiotics without time limitations. Conclusion Herbal medicines can be effective at the first sign of the infection and also for short-term prophylaxis. Using vitamins, trace elementals, and/or sugars is an effective approach in preventing UTIs, and a combination of them with other antibacterial agents shows positive results. Probiotics have great potential for the threat of antibiotic over-usage and the prevalence of antibiotic-resistant microorganisms. This study may be of use in developing the efficient formulation of treatment of UTI.

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Oral probiotics and the female urinary microbiome: a double-blinded randomized placebo-controlled trial

Cited by 26 publications

References 18 publications

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

Therapeutic, Prophylactic, and Functional Use of Probiotics: A Current Perspective

Temporal Dynamics of the Adult Female Lower Urinary Tract Microbiota

Deep insights into urinary tract infections and effective natural remedies

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