Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Abstract

Background Swine influenza A virus (swIAV) is a major concern for the swine industry owing to its highly contagious nature and acute viral disease. Currently, most commercial swIAV vaccines are traditional inactivated virus vaccines. The Lactobacillus plantarum-based vaccine platform is a promising approach for mucosal vaccine development. Oral and intranasal immunisations have the potential to induce a mucosal immune response, which confers protective immunity. The aim of this study was to evaluate the probiotic potential and adhesion ability of three L. plantarum strains. Furthermore, a recombinant L. plantarum strain expressing the head domain of swIAV antigen HA1 was constructed and evaluated for its ability to prevent swIAV infection. Results The three L. plantarum strains isolated from healthy pig faecal samples maintained the highest survival rate when incubated at pH 3 and at bile salt concentration of 0.3%. They also showed high adherence to intestinal cells. All three L. plantarum strains were monitored in live mice, and no major differences in transit time were observed. Recombinant L. plantarum expressed swIAV HA1 protein (pSIP401-HA1-ZN-3) and conferred effective mucosal, cellular and systemic immune responses in the intestine as well as in the upper respiratory airways of mice. In conclusion, the oral and intranasal administration of L. plantarum strain pSIP401-HA1-ZN-3 in mice induced mucosal immunity and most importantly, provided protection against lethal influenza virus challenge. Conclusion In summary, these findings suggest that the engineered L. plantarum strain pSIP401-HA1-ZN-3 can be considered as an alternative approach for developing a novel vaccine during an swine influenza A pandemic.

Mucosal Administration of Lactobacillus casei Surface-Displayed HA1 Induces Protective Immune Responses against Avian Influenza A Virus in Mice

“…4 ). Also, our results are in line with the previous studies in which L. lactis and L. plantarum surface-displayed HA1 antigens conferred the potential protection of mice against the lethal H5N1 and H1N1 virus infections, respectively [ 58 , 59 ].…”

Environmental Cadmium Exposure Perturbs Gut Microbial Dysbiosis in Ducks

“…Roseburia has been shown to have the ability to utilize sugars and produce butyrate [62]. Lactobacillus has been considered one of the most important intestinal beneficial bacteria due to its positive regulatory effect on the intestinal barrier and mucosal immunity [63,64]. Moreover, Lactobacillus has also been reported to improve host growth performance, antioxidant capacity, and digestive enzyme activity [63,65].…”

Roles and Mechanisms of NLRP3 in Influenza Viral Infection

“…Both Bifidobacterium longum BB536 and Bifidobacterium pseudolongum NjM1 significantly alleviate influenza virus infection by inhibiting virus replication [ 88 , 165 ]. Among the Lactobacillus strains, four strains have also been implicated in inhibiting virus replication, including Lactobacillus gasseri TMC0356 [ 166 ], Lactobacillus brevis KB290 [ 167 ], Lactobacillus acidophilus L-92 [ 168 ], and Lactobacillus plantarum DK119 [ 169 , 170 , 171 , 172 , 173 ]. In addition to probiotics, prebiotics have been investigated for their potential to modulate the gut microbiota and improve host responses to influenza infection.…”