Oral mucosa of coeliac disease patients produces antiendomysial and antitransglutaminase antibodies: the diagnostic usefulness of an <i>in vitro</i> culture system

Carroccio, Antonio; Campisi, Giuseppina; Iacono, Giuseppe; Iacono, Oreste Lo; Maresi, Emiliano; Prima, Lidia Di; Compilato, Domenico; Barbaria, F.; Arini, Andrea; Liberto, Chiara Di; Pirrone, Giuseppe; Craxı̀, Antonio; Marco, Vito Di

doi:10.1111/j.1365-2036.2007.03335.x

Cited by 13 publications

(18 citation statements)

References 31 publications

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“…Recent studies have also shown a close association between oral and intestinal mucosa in patients with celiac disease, a complex autoimmune enteropathy induced by dietary gluten [7][8][9]. Consequently, the Ni-patch-induced oral mucosa lesions are probably related to bowel damage that occurs after ingestion of Ni-containing foods that in turn, causes the A Ni patch may also cause development of general reactions such as itching and dermographia, suggesting that the omPT can effectively identify Ni-sensitive patients.…”

Section: Discussionmentioning

confidence: 95%

“…The aim of the present study was to investigate the relationship between intestinal symptoms and Ni intake by a novel oral mucosa patch test (omPT), based on recent studies that have shown a close association between oral and intestinal mucosal compartments in patients with celiac disease, a complex autoimmune enteropathy induced by dietary gluten [7][8][9]. The omPT is described for the first time in the present study.…”

mentioning

confidence: 96%

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Oral Mucosa Patch Test: A New Tool to Recognize and Study the Adverse Effects of Dietary Nickel Exposure

Picarelli

Tola

Vallecoccia

et al. 2010

Biol Trace Elem Res

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On contact with the skin, nickel may cause allergic contact dermatitis, which can be diagnosed by an epicutaneous patch test. Nickel exposure via the intestinal mucosa can induce diarrhea, abdominal pain, and swelling. The aim of the present study was to investigate the relationship between these symptoms and nickel intake by means of a novel oral mucosa patch test. Eighty-six patients with intestinal symptoms related to ingestion of nickel-containing foods were submitted to epicutaneous and oral mucosa patch tests for nickel. All patients with positive oral mucosa patch test results were subject to a low-nickel diet and monitored over time. Skin lesions were observed in 33 out of 86 (38.4%) patients evaluated by the epicutaneous patch test. Mucosal lesions were seen in 53 out of 86 (61.6%) patients given the oral mucosa patch test. After 2 months of a low-nickel diet, 52 out of 53 (98.1%) patients showed an improvement of their symptoms. There is a significant correlation between response time of the oral mucosa patch test and the latency of symptoms after ingestion of nickel-containing foods. Consequently, the oral mucosa patch test can be used to recognize and study the adverse effects of dietary nickel exposure that could be defined as allergic contact mucositis. A low-nickel diet is also shown to be an effective treatment for this condition.

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Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 96%

Oral Mucosa Patch Test: A New Tool to Recognize and Study the Adverse Effects of Dietary Nickel Exposure

Picarelli

Tola

Vallecoccia

et al. 2010

Biol Trace Elem Res

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“…A subcutaneous oral mucosal challenge with gliadins, on the other hand, significantly increased the number of CD4 + and CD8 + T cells in the oral lamina propria of CD patients, but not in nonceliac controls, suggesting an induced oral mucosal immune response to gluten in CD . Cultured oral mucosal and duodenal biopsies from CD patients showed a comparable anti‐TG2 and anti‐endomysal antibody response in the culture media, with a sensitivity between 57 and 100% , likely depending on biopsy size . Saliva as a diagnostic fluid for CD has also been explored.…”

Section: Discussionmentioning

confidence: 99%

Salivary proline‐rich proteins and gluten: Do structural similarities suggest a role in celiac disease?

Tian

Messana

Leffler

et al. 2015

Proteomics Clinical Apps

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Purpose Gluten proteins, the culprits in celiac disease (CD), show striking similarities in primary structure with human salivary proline-rich proteins (PRPs). Both are enriched in pro-line and glutamine residues that often occur consecutively in their sequences. We investigated potential differences in the spectrum of salivary PRPs in health and CD. Experimental design Stimulated salivary secretions were collected from CD patients, patients with refractory CD, patients with gastrointestinal complaints but no CD, and healthy controls. PRP isoforms/peptides were characterized by anionic and SDS-PAGE, PCR, and LC-ESI-MS. Results The gene frequencies of the acidic PRP isoforms PIF, Db, Pa, PRP1, and PRP2 did not differ between groups. At the protein level, PRPs peptides showed minor group differences, but these could not differentiate the CD and/or refractory CDs groups from the controls. Conclusions and clinical relevance This extensive study established that salivary PRPs, despite similarity to gluten proteins, show no apparent correlation with CD and thus will not serve as diagnostic markers for the disease. The structural basis for the tolerance to the gluten-like PRP proteins in CD is worthy of further exploration and may lead to the development of gluten-like analogs lacking immunogenicity that could be used therapeutically.

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“…Anaemia without other clinical signs of intestinal malabsorption is one of the most common extra-intestinal manifestations of CD [37]. CD is frequently diagnosed in patients referred for evaluation for iron deficiency anaemia, which is found in 1.8-14.6% of patients [38].…”

Section: Ferropenic Anaemia and Associated CDmentioning

confidence: 99%

Introductory Chapter: Celiac Disease - An Overview

Rodrigo¹,

Hernández-Lahoz²

2019

Celiac Disease - From the Bench to the Clinic

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Oral mucosa of coeliac disease patients produces antiendomysial and antitransglutaminase antibodies: the diagnostic usefulness of an in vitro culture system

Abstract: SUMMARYBackground Antiendomysial (EmA) and antitransglutaminase (anti-tTG) antibodies are the most specific indirect marker of coeliac disease (CD). It is not known whether the oral mucosa of patients with CD is able to produce these antibodies or not.

Cited by 13 publications

References 31 publications

Oral Mucosa Patch Test: A New Tool to Recognize and Study the Adverse Effects of Dietary Nickel Exposure

Oral Mucosa Patch Test: A New Tool to Recognize and Study the Adverse Effects of Dietary Nickel Exposure

Salivary proline‐rich proteins and gluten: Do structural similarities suggest a role in celiac disease?

Introductory Chapter: Celiac Disease - An Overview

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