Oral microbiota analyses of Saudi sickle cell anemics with dental caries

Alyousef, Yousef M.; Alonaizan, Faisal; Al-Sulaiman, Abdulsalam A.; Aldarwish, Mohammed I.; Alali, Ali; Almasood, Naif N.; Vatte, Chittibabu; Cyrus, Cyril; Habara, Alawi; Koeleman, Bobby P. C.

doi:10.1016/j.identj.2022.06.017

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…It will indicate that the consumption of sweet foods poses a notable risk for the development of dental caries [38]. High carbohydrate consumption combined with poor oral hygiene is considered to be the main cause of severe dental caries [39]. The aforementioned ndings suggest that visual impairment among schoolchildren can have an impact on their learning and practical skills, potentially leading to poorer oral hygiene habits compared to individuals without visual impairments [36].…”

Section: Discussionmentioning

confidence: 99%

Investigation of visually impaired schoolchildren's dental health and salivary microbiota characteristics in Guangzhou, China

feng,

Huang,

Zhao

et al. 2024

Preprint

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Background Reports indicate that the prevalence of visual impairment (VS) among children in China is on the rise. The oral microbiome plays an essential role in maintaining oral homeostasis and health. The primary objective was to examine the oral health and their oral hygiene habits of VS children in Guangzhou. The secondary objective was to explore the differences in the saliva samples microbiota (SM) between HS (health visual children) and VS children. Method 101 VS children were chosen to have their oral health examined and their oral hygiene habits surveyed. The oral microbiota was profiled from saliva samples of 20 VS children and 20 healthy visual (HS) children in the Guangzhou population, and 16s rRNA V3-V4 region were sequenced using the Illumina MiSeq platform. The operational taxonomic units (OTUs) were clustered using QIIME and the statistical analysis was performed by R. Results Oral hygiene is inadequate among the 101 VS children age from 6-16, who have an exceptionally high caries rate of 92.1%. There was no statistically significant distinction observed in the composition of the salivary microbial community between the two groups. HS children had a higher abundance of Firmicutes, Patescibacteria, and Spirochaetes at the phylum level, and Bacilli, Negativicutes, and Saccharimonadia at the classes level, and Streptococcus at the genus level. In contrast, VS children had more Actinobacteria, Bacteroidetes, Proteobacteria, and Fusobacteriota at the phylum level, and Actinobacteria, Bacteroidia, Gammaproteobacteria, Fusobacteriia, and Clostridia at the classes level, and Rothia, Neisseria, Veillonella, Prevotella_7, Actinomyces, Leptotrichia, and lactobacillales at the genus level. Notably, Actinobacteria was significantly positively correlated with gingivitis and dental caries. Additionally, Actinobacteria salivarius, within the phylum Actinobacteria, was substantially more abundant in VS children. Conclusion The study highlights the importance of oral health care for visually impaired schoolchildren in Guangzhou. It provides valuable insights into the salivary microbiota characteristics of this population and suggests potential targets for intervention to improve their oral health status.

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Section: Discussionmentioning

confidence: 99%

Investigation of visually impaired schoolchildren's dental health and salivary microbiota characteristics in Guangzhou, China

feng,

Huang,

Zhao

et al. 2024

Preprint

View full text Add to dashboard Cite

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Genetic Patterns of Oral Cavity Microbiome in Patients with Sickle Cell Disease

Al-Sarraj,

Albiheyri,

Qari

et al. 2024

IJMS

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The Middle Eastern prevalence of sickle cell anemia, a genetic disorder that affects red blood cells, necessitates additional research. On a molecular level, we sought to identify and sort the oral microbiota of healthy individuals and those with sickle cell anemia. Furthermore, it is crucial to comprehend how changes in the genetic makeup of the oral microbiota impact the state of sickle cell anemia. Using next-generation sequencing, the 16S rRNA amplicon was examined using saliva samples from 36 individuals with sickle cell anemia and healthy individuals. These samples were obtained from sickle cell anemia patients (18 samples) and healthy control participants (controls, 18 samples). Various analyses are conducted using bioinformatic techniques to identify distinct species and their relative abundance. Streptococcus, followed by Fusobacterium nucleatum, Prevotella, and Veillonella were the most prevalent genera of bacteria in the saliva of the SCA and non-SCA individuals according to our findings. Rothia mucilaginosa, Prevotella scoposa, and Veillonella dispar species were the dominant species in both sickle cell anemia and non-sickle cell anemia subjects. Streptococcus salivarius, Actinomyces graevenitzii, Actinomyces odontolyticus, and Actinomyces georgiae spp. were the most prevalent bacterial spp. in the studied SCA cases. The sequencing of the 16S rRNA gene yielded relative abundance values that were visualized through a heatmap analysis. Alterations in the oral microflora’s constitution can significantly affect the susceptibility of sickle cell anemia patients to develop more severe health complications. Salivary diagnosis is a potential tool for predicting and preventing oral microbiome-related diseases in the future.

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Oral microbiota analyses of Saudi sickle cell anemics with dental caries

Cited by 2 publications

References 34 publications

Investigation of visually impaired schoolchildren's dental health and salivary microbiota characteristics in Guangzhou, China

Investigation of visually impaired schoolchildren's dental health and salivary microbiota characteristics in Guangzhou, China

Genetic Patterns of Oral Cavity Microbiome in Patients with Sickle Cell Disease

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