Oral microbiome alterations of healthy volunteers with proton pump inhibitor

Mishiro, Tsuyoshi; Oka, Koichiro; Kuroki, Yasutoshi; Takahashi, Motomichi; Tatsumi, Kasumi; Saitoh, Takayuki R.; Tobita, Hiroshi; Ishimura, Norihisa; Sato, Shuichi; Ishihara, Shunji; Sekine, Joji; Wada, Koichiro; Kinoshita, Yoshikazu

doi:10.1111/jgh.14040

Cited by 62 publications

(64 citation statements)

References 38 publications

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“…Our study is consistent with the notion that H. pylori in the stomach has little or no effect on the bacterial diversity of the oral cavity [20]. The Shannon diversity index, ACE richness index, and Chao 1 richness estimator all declined after eradication of H. pylori compared to the baseline samples (p < 0.05), which was consistent with the prior studies that use of PPI and antibiotics may affect the oral microbiome [21,22].…”

Section: Discussionsupporting

confidence: 92%

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

Liang

Lü

2020

BMC Oral Health

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Background: There have been reports of Helicobacter pylori (H. pylori) in the oral cavity and it has been suggested that the oral cavity may be a reservoir for H. pylori reflux from the stomach. High-throughput sequencing was used to assess the structure and composition of oral microbiota communities in individuals with or without confirmed H. pylori infection. Methods: Saliva samples were obtained from 34 H. pylori infected and 24 H. pylori uninfected subjects. Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatics analysis. Saliva sampling was repeated from 22 of the 34 H. pylori infected subjects 2 months after H. pylori eradication. Results: High-quality sequences (2,812,659) clustered into 95,812 operational taxonomic units (OTUs; 97% identity). H. pylori was detected in the oral cavity in infected (12/34), uninfected (11/24) and eradicated (15/22) subjects by technique of high-throughput sequencing, occupying 0.0139% of the total sequences. Alpha diversity of H. pylori infected subjects was similar to that of uninfected subjects (

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Section: Discussionsupporting

confidence: 92%

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

Liang

Lü

2020

BMC Oral Health

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“…Our study is consistent with the notion that H. pylori in the stomach has little or no effect on the bacterial diversity of the oral cavity (20). The Shannon diversity index, ACE richness index, and Chao 1 richness estimator all declined after eradication of H. pylori compared to the baseline samples (p<0.05), which was consistent with the prior studies that use of PPIs and antibiotics may affect the oral microbiome (21, 22).…”

Section: Discussionsupporting

confidence: 89%

The Salivary Microbiome: Analysis of by Pyrosequencing and the Relationship with Helicobacter pylori Infection

Liang

Lü

2018

Preprint

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Words count of abstract: 249 15 Words count of text: 3806 16 17 2 Abstracts 18Backgrounds: There have been reports of Helicobacter pylori (H. pylori) in the oral cavity and it has 19 been suggested that the oral cavity may be a reservoir for H. pylori reflux from the stomach. 20Objectives: High-throughput pyrosequencing was used to assess the structure and composition of oral 21 microbiota communities in individuals with or without confirmed H. pylori infection. 22 Methods: Saliva samples were obtained from 34 H. pylori infected and 24 H. pylori uninfected 23 subjects. Bacterial genomic DNA was extracted and examined by pyrosequencing by amplification of 24 the 16S rDNA V3-V4 hypervariable regions followed by bioinformatics analysis. Saliva sampling was 25 repeated from 22 of the 34 H. pylori infected subjects 2 months after H. pylori eradication. 26 Results: High-quality sequences (2,812,659) clustered into 95,812 operational taxonomic units (OTUs; 27 97% identity), representing 440 independent species belonging to 138 genera, 68 families, 36 orders, 28 21 classes, and 11 phyla. Species richness (alpha diversity) of H. pylori infected subjects was similar to 29 that of uninfected subjects. Eradication treatment decreased saliva bacterial diversity. Beta diversity 30analysis showed that the salivary microbial community structure differed between H. pylori infected 31 and uninfected subjects both before and after H. pylori eradication. 32Conclusions: Salivary microbiota diversity was similar in H. pylori infected and uninfected individuals. 33Antibiotic therapy was associated with a decline in salivary bacterial diversity. Both H. pylori infection 34 and its eradication caused the oral microbiota alterations in community and structure. The present of H. Importance 39The oral cavity plays a vital role in Helicobacter pylori transmission among human. 40High-throughput pyrosequencing of the 16S rDNA V3-V4 hypervariable regions was used to assess the 41 structure and composition of oral microbiota communities in individuals with or without confirmed 42 Helicobacter pylori infection. We show that both Helicobacter pylori infection and eradication cause 43 microbiota alterations in the oral microbiota. Prior studies report detection of Helicobacter pylori in the 44 oral cavity by polymerase chain reaction. We show that the presence of Helicobacter pylori in the oral 45 cavity is unrelated with its infection status in the stomach. 46 47 4 48 Helicobacter pylori (H. pylori) is a Gram-negative bacterium that colonizes the human gastric 49 epithelium. It belonged to Helicobacter genus, Helicobacteraceae family, Campylobacterales order, 50 Epsilonproteobacteria class, and Proteobacteria phyla. H. pylori infection is characterized by mucosal 51 inflammation (gastritis) and may result in peptic ulcer disease or gastric adenocarcinoma (1). H. pylori 52 is transmitted between humans by a variety of routes including gastro-oral and fecal-oral mechanisms 53 that include contaminated water and food. It has also been postulated t...

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“…A prospective investigation in 10 healthy adults showed that a 4‐week course of esomeprazole (20 mg once daily) decreased species diversity of saliva microbiota, while not significantly affecting species richness and community diversity of saliva or periodontal pocket microbiota. At the genus level, PPIs reduced Veillonella , Neisseria and Haemophilus abundances in the saliva and increased Fusobacterium and Leptotrichia in the periodontal pocket (Tables and ).…”

Section: Resultsmentioning

confidence: 99%

“…In a cohort of 24 elderly, healthy individuals, a 14‐day course of omeprazole (20 mg once daily) did not affect faecal bacterial richness or diversity, but significantly reduced the abundance of Lachnospiraceae, Erysipelotrichaceae and Bifidobacteriaceae, while increasing the abundance of Streptococcaceae . Mishiro et al equally observed increased abundance of faecal Streptococcus levels after 4‐weeks of esomeprazole (20 mg once daily) …”

Section: Resultsmentioning

confidence: 99%

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Systematic review: the effects of proton pump inhibitors on the microbiome of the digestive tract—evidence from next‐generation sequencing studies

Macke

Schulz

Koletzko

et al. 2020

Aliment Pharmacol Ther

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Background: Proton pump inhibitors (PPI) are widely used to treat acid-related disorders of the upper gastrointestinal tract. However, large observational studies have raised concerns about PPI-associated adverse events. In recent years, data from next-generation sequencing studies suggested that PPIs affect the composition of the intestinal microbiota, while a balanced gut microbiome is essential for maintaining health.Aim: To review the available evidence from next-generation sequencing studies on the effect of PPIs on the intestinal microbiome and to discuss possible implications of PPI-induced dysbiosis in health and disease.Methods: A systematic review was conducted following the recommendations of the Preferred Reporting Items for Systematic reviews and Meta-Analyses statement. A PubMed query yielded 197 results. 19 publications met the prespecified eligibility criteria.Results: Twelve observational study cohorts with 708 PPI users and 11 interventional cohorts with 180 PPI users were included in the review. In most studies, PPI treatment did not affect microbiological richness and diversity, but was associated with distinct taxonomic alterations: In the upper gastrointestinal tract, PPI users showed overgrowth of orally derived bacteria, mostly Streptococcaceae (findings based on six independent cohorts with 126 PPI users). In faecal samples, PPIs increased multiple taxa from the orders Bacillales (eg, Staphylococcaceae), Lactobacillales (eg, Enterococcaceae, Lactobacillaceae, Streptococcaceae) and Actinomycetales (eg, Actinomycetaceae, Micrococcaceae), the families Pasteurellaceae and Enterobacteriaceae and the genus Veillonella. Taxa decreased by PPIs include Bifidobacteriaceae, Ruminococcaceae, Lachnospiraceae and Mollicutes (findings in faecal samples based on 19 independent cohorts with 790 PPI users). Conclusion: PPI use is associated with moderate alterations to upper and distal gut microbiota. The available data suggest that PPI-induced hypochlorhydria facilitates colonization of more distal parts of the digestive tract by upper gastrointestinal microbiota. MACKE Et Al 1 | INTRODUC TI ON: G A S TRI C ACID INHIB ITI ON AND ADVER S E EFFEC TS OF PPIS Gastric acid eliminates microorganisms, promotes the digestive process and facilitates the absorption of iron, calcium and vitamin B12. However, gastric acid also contributes to the development of mild and severe gastroduodenal pathologies. Proton pump inhibitor (PPIs) are the most effective therapy of acid-related diseases and are among the most commonly used drugs. 1 While generally considered a safe therapy, PPIs have been associated with numerous adverse effects in population-based observational studies conducted in recent years. These include cardiovascular and kidney disease, micronutrient deficiencies and osteoporosis, cognitive impairment and death. 2Most studies are retrospective and not hypothesis-driven in design, might be subject to significant confound and thus they cannot establish causality. 2,3 This is also the case for a recent study on ...

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Oral microbiome alterations of healthy volunteers with proton pump inhibitor

Abstract: These results suggest that PPIs cause both oral and gut microbiota alterations.

Cited by 62 publications

References 38 publications

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

The Salivary Microbiome: Analysis of by Pyrosequencing and the Relationship with Helicobacter pylori Infection

Systematic review: the effects of proton pump inhibitors on the microbiome of the digestive tract—evidence from next‐generation sequencing studies

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