Oral microbial influences on oral mucositis during radiotherapy treatment of head and neck cancer

Vesty, Anna; Gear, Kim; Biswas, Kristi; Mackenzie, Brett Wagner; Taylor, Michael W.; Douglas, Richard

doi:10.1007/s00520-019-05084-6

Cited by 50 publications

(50 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Core salivary bacterial genera, including Streptococcus, remain stable during radiotherapy 31 . However, subsequent hyposalivation and xerostomia, reported by 10 of the 13 patients in this study, may compromise the ability of probiotic strains to disseminate and colonise the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

Randomised, double-blind, placebo-controlled trial of oral probiotic Streptococcus salivarius M18 on head and neck cancer patients post-radiotherapy: a pilot study

Vesty

Gear

Boutell

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Xerostomia detrimentally affects the oral health of many head and neck cancer patients who undergo radiotherapy. Its sequelae become an ongoing burden for patients that often manifest as periodontal disease and dental decay. Bacteria play a major role in the pathogenesis of these conditions and here we explore the use of an oral probiotic to beneficially modulate the oral bacterial community postradiotherapy. In this pilot study, a four-week intervention with oral probiotic lozenges containing Streptococcus salivarius M18 was trialled in seven patients. Post-intervention changes in oral health and in the composition of the plaque and saliva bacterial communities were compared with six patients in a placebo group. An improvement in periodontal screening and plaque index scores was observed in both groups after the intervention period. The oral probiotic lozenges did not significantly impact bacterial community composition or diversity, nor did the probiotic lozenges increase the relative sequence abundance of ZOTU_1 (the probiotic-associated sequence assigned to S. salivarius) detected in the samples. Network analyses suggest negative interactions occurred between ZOTU_1 and species from the periopathogenic genera Campylobacter, Fretibacterium, Selenomonas and Treponema but further investigation is required to more fully understand the beneficial properties of this oral probiotic. Head and neck cancer describes a group of malignant tumours that often require radiotherapy as part of the treatment approach. Due to the close proximity of the salivary glands to the radiation portal, many head and neck cancer patients sustain permanent radiation-induced damage to the salivary glands during radiotherapy 1,2. Subsequently, salivary gland function is reduced, resulting in chronic hyposalivation and xerostomia (dry mouth) that causes considerable discomfort, compromises mastication and speech, increases the risk of oral health complications and decreases the quality of life for many patients 1,3,4. Hyposalivation and loss of salivary buffering capacity lead to a decrease in the pH of saliva and a shift to acidogenic and cariogenic bacteria, including an increase the in abundance of Streptococcus mutans and Lactobacillus spp. 5-7. These effects, combined with a decrease in the mechanical flushing mechanism of saliva to reduce plaque build-up, leave patients prone to post-radiotherapy gingivitis, periodontal disease and rampant dental decay 2,3,8. Oral probiotics, primarily those containing lactobacilli, have been successfully shown to decrease the abundance of periopathogens in sub-and supra-gingival plaque, significantly improve plaque index and periodontal

show abstract

Section: Discussionmentioning

confidence: 99%

Randomised, double-blind, placebo-controlled trial of oral probiotic Streptococcus salivarius M18 on head and neck cancer patients post-radiotherapy: a pilot study

Vesty

Gear

Boutell

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tongue cancer, or squamous cell carcinoma of the tongue, is one of the most common malignant tumors of the head and neck, as well as being a common oral cancer. 23 Tongue cancer can be divided into 2 types: tongue body cancer in the anterior 2/3 part and the tongue root cancer in the posterior 1/3 part. 24 At present, surgical treatment, radiotherapy, chemotherapy, and targeted treatment are mainly used to treat tongue cancer.…”

Section: Discussionmentioning

confidence: 99%

Silencing of SPP1 Suppresses Progression of Tongue Cancer by Mediating the PI3K/Akt Signaling Pathway

Zhang

Lai

et al. 2020

Technol Cancer Res Treat

View full text Add to dashboard Cite

Background: In the present study, we aimed to find an effective target for the treatment of tongue cancer using gene chip screening and signal pathway research. Methods: We used microarray screening and gene expression profile analyses to find important differentially expressed genes in tongue cancer. We constructed a protein-protein interaction network, and used enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes to screen for important genes. We then silenced the genes of interest in SCC154 cells to study the relationship with the Phosphatidylinositol 3-kinase/Akt signal pathway. Western blot analyses, the 3-(4,5Dimethylthiazol-yl)-2,5Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) test, and immunofluorescence assays were used to compare the expression levels of Phosphatidylinositol 3-kinase/Akt signal pathway-related proteins, cell viability, and cell proliferation ability in normal SCC154 cells, Si-RNA SCC154 cells, and gene-silenced SCC154 cells. The scratch test, Transwell test, and western blotting were used to determine migration, invasion, and carcinogenesis. Results: Using GSE9844, GSE13601, and GSE31056 gene chips, we identified 93 upregulated genes and 76 downregulated genes in tongue cancer. Using the protein-protein interaction network and Kyoto Encyclopedia of Genes and Genomes enrichment analyses, we further identified 47 differentially expressed genes. Using Kaplan-Meier plotter online tools, we also identified 3 genes (SPP1, Recombinant Human Secreted Phosphoprotein 1; PLAU, plasminogen activator urinary; and APP, amyloid precursor protein). Compared with normal SCC154 cells and Si-RNA control SCC154 cells, the expressions of Phosphatidylinositol 3-kinase/Akt pathway proteins in si-SPP1 SCC154 cells were significantly decreased (*P < 0.05), and the protein activities and proliferation abilities were also significantly decreased (*P < 0.05), while the migration ability, invasion ability, and cancer forming ability were significantly increased (*P < 0.05). Conclusion: Inhibition of the SPP1 gene may have a therapeutic effect on tongue cancer, and could be an effective target for the treatment of this disorder.

show abstract

“…Infection complicates the damage of mucositis. The abundance of a variety of gram-negative bacteria ( Fusobacterium, Haemophilus, Tannerella, Porphyromonas, and Eikenella ) in the oral mucosa may influence susceptibility of patients to RIOM ( 70 ). Therefore, oral health intervention is necessary.…”

Section: Clinical Intervention For Riom and Malnutrition In Hnc Patiementioning

confidence: 99%

The Effects of Early Nutritional Intervention on Oral Mucositis and Nutritional Status of Patients With Head and Neck Cancer Treated With Radiotherapy

Zheng

Zhao

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Radiation-induced oral mucositis (RIOM) is a common side effect after radiotherapy (RT) in head and neck cancer (HNC) patients. RIOM patients with severe pain have difficulty in eating, which increases the incidence of malnutrition and affects patients’ quality of life and the process of RT. The mechanism of RIOM is not fully understood, and inflammatory response and oxidative stress appear to be important for RIOM occurrence and development. The nutritional status of patients is very important for their RT tolerance and recovery. Malnutrition, which can lead to anemia, low protein, decreased immunity and other problems, is an important clinical factor affecting tumor progression and treatment. Recent studies have shown that early nutritional intervention can ameliorate oral mucositis and nutritional status of patients with HNC. However, in clinical practice, early nutritional intervention for patients with HNC is not a conventional intervention strategy. Therefore, this review summarized the possible pathogenesis of RIOM, commonly used assessment tools for malnutrition in patients, and recent studies on the effects of early nutritional interventions on RIOM and nutritional status of patients with HNC. We hope to provide the basis and reference for the clinical application of early nutritional intervention models.

show abstract

Oral microbial influences on oral mucositis during radiotherapy treatment of head and neck cancer

Cited by 50 publications

References 35 publications

Randomised, double-blind, placebo-controlled trial of oral probiotic Streptococcus salivarius M18 on head and neck cancer patients post-radiotherapy: a pilot study

Randomised, double-blind, placebo-controlled trial of oral probiotic Streptococcus salivarius M18 on head and neck cancer patients post-radiotherapy: a pilot study

Silencing of SPP1 Suppresses Progression of Tongue Cancer by Mediating the PI3K/Akt Signaling Pathway

The Effects of Early Nutritional Intervention on Oral Mucositis and Nutritional Status of Patients With Head and Neck Cancer Treated With Radiotherapy

Contact Info

Product

Resources

About