Oral malodorous compound triggers mitochondrial-dependent apoptosis and causes genomic DNA damage in human gingival epithelial cells

Calenic, Bogdan; Yaegaki, Ken; Murata, Takatoshi; Imai, Toshio; Aoyama, Izumi; Sato, Tsutomu; Ii, Hongyi

doi:10.1111/j.1600-0765.2008.01199.x

Cited by 52 publications

(68 citation statements)

References 39 publications

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“…D. oralis encodes several sulfatases that could liberate inorganic sulfate from GAGs, potentially playing an active role in matrix destruction (93). In addition, the hydrogen sulfide produced by D. oralis during sulfate respiration can lead to apoptosis in keratinocytes (82,94) and further elicits a proinflammatory response in oral cells (95,96). These features illustrate the capacity of D. oralis to create a dysbiotic oral environment and actively contribute to the pathogenesis of periodontal disease in a novel and compounded way not described for other oral pathogens so far.…”

mentioning

confidence: 99%

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

Cross

Chirania

Xiong

et al. 2018

mBio

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The human oral microbiota encompasses representatives of many bacterial lineages that have not yet been cultured. Here we describe the isolation and characterization of previously uncultured Desulfobulbus oralis, the first humanassociated representative of its genus. As mammalian-associated microbes rarely have free-living close relatives, D. oralis provides opportunities to study how bacteria adapt and evolve within a host. This sulfate-reducing deltaproteobacterium has adapted to the human oral subgingival niche by curtailing its physiological repertoire, losing some biosynthetic abilities and metabolic independence, and by dramatically reducing environmental sensing and signaling capabilities. The genes that enable free-living Desulfobulbus to synthesize the potent neurotoxin methylmercury were also lost by D. oralis, a notably positive outcome of host association. However, horizontal gene acquisitions from other members of the microbiota provided novel mechanisms of interaction with the human host, including toxins like leukotoxin and hemolysins. Proteomic and transcriptomic analysis revealed that most of those factors are actively expressed, including in the subgingival environment, and some are secreted. Similar to other known oral pathobionts, D. oralis can trigger a proinflammatory response in oral epithelial cells, suggesting a direct role in the development of periodontal disease.IMPORTANCE Animal-associated microbiota likely assembled as a result of numerous independent colonization events by free-living microbes followed by coevolution with their host and other microbes. Through specific adaptation to various body sites and physiological niches, microbes have a wide range of contributions, from beneficial to disease causing. Desulfobulbus oralis provides insights into genomic and physiological transformations associated with transition from an open environment to a host-dependent lifestyle and the emergence of pathogenicity. Through a multifaceted mechanism triggering a proinflammatory response, D. oralis is a novel periodontal pathobiont. Even though culture-independent approaches can provide insights into the potential role of the human microbiome "dark matter," cultivation and experimental characterization remain important to studying the roles of individual organisms in health and disease.KEYWORDS evolution, genome analysis, oral microbiology, periodontitis, proteomics

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confidence: 99%

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

Cross

Chirania

Xiong

et al. 2018

mBio

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“…67 A number of studies showed the positive relation between progression of periodontal disease and VSC amount. 35,36 Morita and Wang 68 found a significant relation between the severity of periodontal disease and VSC amount in breath. They discovered that VSC value was measured lower within people who are provided with periodontal treatment than the ones who are not treated.…”

Section: Discussionmentioning

confidence: 97%

The Effect of Diode Laser as an Adjunct to Periodontal Treatment on Clinical Periodontal Parameters and Halitosis: A Randomized Controlled Clinical Trial

Hatipoğlu¹,

Aytekin²,

Daltaban³

et al. 2017

Cumhuriyet Dental Journal

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“…The initial apical migration of junctional epithelium in lipopolysaccharideinduced experimental periodontitis appeared to occur simultaneously with the apoptosis of periodontal ligament fibroblasts. [25][26][27] It was therefore suggested that the apoptosis-related detachment of connective tissue may cause the migration of junctional epithelium. Callenic and others found there were two main mechanisms in the apoptotic process involve activation of an intrinsic pathway, in which the mitochondrion plays a central role, and activation of an extrinsic pathway, involving a receptor-ligand-mediated mechanism.…”

Section: Discussionmentioning

confidence: 99%

Volatile sulphur compounds elimination: A new insight in periodontal treatment

Setiawatie

Hudyono

2011

Dent. J. (Majalah Kedokteran Gigi)

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Oral malodorous compound triggers mitochondrial-dependent apoptosis and causes genomic DNA damage in human gingival epithelial cells

Abstract: Hydrogen sulfide induces apoptosis in human gingival epithelial cells by activating the mitochondrial pathway.

Cited by 52 publications

References 39 publications

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont,Desulfobulbus oralis

The Effect of Diode Laser as an Adjunct to Periodontal Treatment on Clinical Periodontal Parameters and Halitosis: A Randomized Controlled Clinical Trial

Volatile sulphur compounds elimination: A new insight in periodontal treatment

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