Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice

Kihl, Pernille; Krych, Łukasz; Deng, Ling; Kildemoes, Anna Overgaard; Laigaard, Ann; Hansen, Lars Hestbjerg; Hansen, Camilla Hartmann Friis; Buschard, Karsten; Nielsen, Dennis Sandris; Hansen, Axel Kornerup

doi:10.1155/2019/1649279

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…LPSs can be involved in diabetes development, because they lead to the impairment of pancreatic β-cell function and increase the level of pro-inflammatory cytokines [ 97 ]. In mouse models, an oral injection of E. coli LPS improved local immunity, while an intraperitoneal injection of E. coli LPS improved the autoimmune response and decreased the incidence of T1D [ 98 , 99 ]. However, it is difficult to conclude whether microbial alteration is causal or consequential for T1D development.…”

Section: The Potential Role Of the Gut Microbiota In The Development Of T1dmentioning

confidence: 99%

The Role of the Gut Microbiota in the Pathogenesis of Diabetes

Bielka

Przezak

Pawlik

2022

IJMS

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Diabetes mellitus is a significant clinical and therapeutic problem because it can lead to serious long-term complications. Its pathogenesis is not fully understood, but there are indications that dysbiosis can play a role in the development of diabetes, or that it appears during the course of the disease. Changes in microbiota composition are observed in both type 1 diabetes (T1D) and type 2 diabetes (T2D) patients. These modifications are associated with pro-inflammation, increased intestinal permeability, endotoxemia, impaired β-cell function and development of insulin resistance. This review summarizes the role of the gut microbiota in healthy individuals and the changes in bacterial composition that can be associated with T1D or T2D. It also presents new developments in diabetes therapy based on influencing the gut microbiota as a promising method to alter the course of diabetes. Moreover, it highlights the lacking data and suggests future directions needed to prove the causal relationship between dysbiosis and diabetes, both T1D and T2D.

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Section: The Potential Role Of the Gut Microbiota In The Development Of T1dmentioning

confidence: 99%

The Role of the Gut Microbiota in the Pathogenesis of Diabetes

Bielka

Przezak

Pawlik

2022

IJMS

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“…LPS also participates in the regulation of autoimmunity, most of which are Escherichia coli LPS involved in suppressing innate immune signals, but Bacteroides dorei LPS does not show significant improvement in T1D incidence[ 94 ]. In a recent study, intraperitoneal injection of Escherichia coli LPS in T1D mice showed a decrease in the incidence of T1D and an improved autoimmune response[ 94 ], while another study of NOD mice that received oral injection of Escherichia coli LPS also demonstrated an improvement in local immunity[ 95 ]. The concept that the pathogenesis of T1D is affected by gut microbiota has been well established in mouse models, but human studies on the microbiome in T1D are still few and far between to provide convincing evidence.…”

Section: Gut Microbiota and T1dmentioning

confidence: 99%

Gut microbiota and diabetes: From correlation to causality and mechanism

Stirling

Yang

et al. 2020

WJD

107

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In this review, we summarize the recent microbiome studies related to diabetes disease and discuss the key findings that show the early emerging potential causal roles for diabetes. On a global scale, diabetes causes a significant negative impact to the health status of human populations. This review covers type 1 diabetes and type 2 diabetes. We examine promising studies which lead to a better understanding of the potential mechanism of microbiota in diabetes diseases. It appears that the human oral and gut microbiota are deeply interdigitated with diabetes. It is that simple. Recent studies of the human microbiome are capturing the attention of scientists and healthcare practitioners worldwide by focusing on the interplay of gut microbiome and diabetes. These studies focus on the role and the potential impact of intestinal microflora in diabetes. We paint a clear picture of how strongly microbes are linked and associated, both positively and negatively, with the fundamental and essential parts of diabetes in humans. The microflora seems to have an endless capacity to impact and transform diabetes. We conclude that there is clear and growing evidence of a close relationship between the microbiota and diabetes and this is worthy of future investments and research efforts.

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“…It is known that Akkermansia muciniphila, belonging to the genera Akkermansia, is a mucindegrader bacterium and it has been reported to have both regulatory and inflammatory properties (64). The increase of this genus has been reported in other autoimmune pathologies, such as multiple sclerosis (64,65) and type 1 diabetes patients (66). The reasons of this increase can be found in its dual functions: it can increase to compensate for the imbalance in gut microbiota composition or it can contribute itself to the disequilibrium.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Bifidobacterium Administration on Experimental Autoimmune Myasthenia Gravis in Lewis Rats

et al. 2019

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Beneficial effects of probiotics on gut microbiota homeostasis and inflammatory immune responses suggested the investigation of their potential clinical efficacy in experimental models of autoimmune diseases. Indeed, administration of two bifidobacteria and lactobacilli probiotic strains prevented disease manifestations in the Lewis rat model of Myasthenia Gravis (EAMG). Here, we demonstrate the clinical efficacy of therapeutic administration of vital bifidobacteria (i.e., from EAMG onset). The mechanisms involved in immunomodulation were investigated with ex vivo and in vitro experiments. Improvement of EAMG symptoms was associated to decreased anti-rat AChR antibody levels, and differential expression of TGFβ and FoxP3 immunoregulatory transcripts in draining lymph nodes and spleen of treated-EAMG rats. Exposure of rat bone marrow-derived dendritic cells to bifidobacteria or lactobacilli strains upregulated toll-like receptor 2 mRNA expression, a key molecule involved in bacterium recognition via lipotheicoic acid. Live imaging experiments of AChR-specific effector T cells, co-cultured with BMDCs pre-exposed to bifidobacteria, demonstrated increased percentages of motile effector T cells, suggesting a hindered formation of TCR-peptide-MHC complex. Composition of gut microbiota was studied by 16S rRNA gene sequencing, and α and β diversity were determined in probiotic treated EAMG rats, with altered ratios between Tenericutes and Verrucomicrobia (phylum level), and Ruminococcaceae and Lachnospiraceae (family level). Moreover, the relative abundance of Akkermansia genus was found increased compared to healthy and probiotic treated EAMG rats. In conclusion, our findings confirms that the administration of vital bifidobacteria at EAMG onset has beneficial effects on disease progression; this study further supports preclinical research in human MG to evaluate probiotic efficacy as supplementary therapy in MG.

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Oral LPS Dosing Induces Local Immunological Changes in the Pancreatic Lymph Nodes in Mice

Cited by 10 publications

References 46 publications

The Role of the Gut Microbiota in the Pathogenesis of Diabetes

The Role of the Gut Microbiota in the Pathogenesis of Diabetes

Gut microbiota and diabetes: From correlation to causality and mechanism

Therapeutic Effect of Bifidobacterium Administration on Experimental Autoimmune Myasthenia Gravis in Lewis Rats

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