Oral L-glutamine pretreatment attenuates skeletal muscle atrophy induced by 24-h fasting in mice

Vasconcelos, Diogo Antônio Alves de; Giesbertz, Pieter; Souza, Diego Ribeiro de; Vitzel, Kaio Fernando; Abreu, Phablo; Marzuca‐Nassr, Gabriel Nasri; Fortes, Marco Aurélio Salomão; Murata, Gilson Masahiro; Hirabara, Sandro Massao; Curi, Rui; Daniel, Hannelore; Pithon-Curi, Tânia Cristina

doi:10.1016/j.jnutbio.2019.05.010

Cited by 25 publications

(17 citation statements)

References 71 publications

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“…Results were normalized to sample total protein content as determined by Ponceau S staining and presented as arbitrary units. In our previous studies [6,28,29] and in the others [28][29][30][31] the use of the same procedure was reported. Previous in vitro studies reported no changes in the content of total insulin signaling proteins [32][33][34][35][36] and highlighted changes in the phosphorylated proteins.…”

Section: Calculations Of Uptake and Export Of Amino Acidsmentioning

confidence: 81%

“…Western blotting analysis C 2 C 12 myotubes were scraped off the plates and homogenized in lysis buffer containing protease and phosphatase inhibitors. The same procedure is in our previous studies [6,28,29]. Antibodies were diluted (1:1000) in Tris-buffered saline with 0.1% Tween 20 (TBST) containing 5% BSA (bovine serum albumin).…”

Section: Calculations Of Uptake and Export Of Amino Acidsmentioning

confidence: 99%

“…A loss of skeletal mass is associated with catabolic conditions such as diabetes, cancer, sepsis, and fasting, and it links to a reduced intracellular L-glutamine content in skeletal muscle [1][2][3][4][5][6]. In muscle wasting states, proteins are degraded raising intracellular levels of essential amino acids (EAAs), such as L-leucine, L-isoleucine, L-valine, L-phenylalanine, and L-methionine [6,7]. Proteolysis is also associated with an increase in transcript levels of EAAs transporters that secondarily leads to a further elevation of these amino acids uptake (GAAC -General Amino Acids Control) by the cells [8].…”

Section: Introductionmentioning

confidence: 99%

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Myotube Protein Content Associates with Intracellular L-Glutamine Levels

Vasconcelos

Giesbertz

Murata

et al. 2019

Cell Physiol Biochem

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Section: Calculations Of Uptake and Export Of Amino Acidsmentioning

confidence: 81%

Section: Calculations Of Uptake and Export Of Amino Acidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Myotube Protein Content Associates with Intracellular L-Glutamine Levels

Vasconcelos

Giesbertz

Murata

et al. 2019

Cell Physiol Biochem

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“…In skeletal muscle, Glu is converted by glutamine synthetase into Gln which can be released from skeletal muscle with Val into the blood, followed by the liver, intestines, and other tissues, maintaining the balance of blood glucose as the substrate of gluconeogenesis (Newsholme et al., 2011). A recent study has proven that both protein consumption and atrophy in skeletal muscle can be relieved by Gln supplementation (de Vasconcelos et al., 2019). Ala, which accumulates at high levels in skeletal muscle, plays a major role as an energy source.…”

Section: Discussionmentioning

confidence: 99%

Zanthoxylum alkylamides improve amino acid metabolism in type 2 diabetes mellitus rats

et al. 2020

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To investigate the effect of Zanthoxylum alkylamides (ZA) on amino acid metabolism of type 2 diabetes mellitus (T2DM), the present study was performed with T2DM rats model induced with high fat and sugar fodder combined with low-dose of streptozotocin. ZA were fed to rats at three different doses of 2, 4, and 8 mg/kg for 28 days and metformin was fed to rats at 135 mg/kg as positive control. The results showed that compared with the normal control, the amino acid levels and the expression of related carrier genes were disordered in T2DM rats. Compared with the model, different doses of ZA could significantly resist (p < .05) the decrease in body weight of T2DM rats and improve hyperglycemia, with the best result observed with the high dose (8 mg/kg). Different doses of ZA could ameliorate the levels of 19 kinds of amino acid in the plasma, jejunum, liver, and skeletal muscle of T2DM rats by regulating the expression of related amino acid transporters including LAT1, SNAT2, CAT1, et al. to thereby ameliorating amino acid metabolism disorder in T2DM rats. Practical applications Previous studies showed that Zanthoxylum alkylamides (ZA) could promote the amino acid metabolism in the jejunum of healthy SD rats, improve protein metabolism disorder of type 1 diabetic rats, and also reduce the risk of metabolic syndrome in fat rats model. Herein, we investigated the effect of ZA on amino acid metabolism in type 2 diabetes mellitus (T2DM) rats. The results indicated that ZA could remarkably improve the abnormal expression of amino acid carriers in the jejunum, liver, and skeletal muscle, thereby ameliorating the disorder of amino acid metabolism in the plasma, jejunum, liver, and skeletal muscle of T2DM rats. Therefore, ZA are potential antidiabetic food/medicine product for the T2DM treatment.

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“…L-glutamine improves C2C12 cells differentiation and prevents TNF-α-induced myotube atrophy via reducing p38 mitogen-activated protein kinase (MAPK) signal transduction [35]. Oral L-glutamine pretreatment attenuates 24-h fasting-induced muscle atrophy in C57BL/6 mice [36]. Moreover, glutamine supplementation prevents glucocorticoid-induced SD rats skeletal muscle atrophy and increases muscle mass, partially through inhibiting the expression of myostatin.…”

Section: Protein Amino Acid and Peptidesmentioning

confidence: 99%

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

Wang

Liu²,

Quan

et al. 2021

Nutrients

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Imbalance of protein homeostasis, with excessive protein degradation compared with protein synthesis, leads to the development of muscle atrophy resulting in a decrease in muscle mass and consequent muscle weakness and disability. Potential triggers of muscle atrophy include inflammation, malnutrition, aging, cancer, and an unhealthy lifestyle such as sedentariness and high fat diet. Nutraceuticals with preventive and therapeutic effects against muscle atrophy have recently received increasing attention since they are potentially more suitable for long-term use. The implementation of nutraceutical intervention might aid in the development and design of precision medicine strategies to reduce the burden of muscle atrophy. In this review, we will summarize the current knowledge on the importance of nutraceuticals in the prevention of skeletal muscle mass loss and recovery of muscle function. We also highlight the cellular and molecular mechanisms of these nutraceuticals and their possible pharmacological use, which is of great importance for the prevention and treatment of muscle atrophy.

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Oral L-glutamine pretreatment attenuates skeletal muscle atrophy induced by 24-h fasting in mice

Cited by 25 publications

References 71 publications

Myotube Protein Content Associates with Intracellular L-Glutamine Levels

Myotube Protein Content Associates with Intracellular L-Glutamine Levels

Zanthoxylum alkylamides improve amino acid metabolism in type 2 diabetes mellitus rats

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

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