Oral intake of α‑glucosyl‑hesperidin ameliorates selenite‑induced cataract formation

Abstract

Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti-cataract effects of α-glucosyl hesperidin in vivo and in vitro using a selenite-induced cataract model. SD rats (age, 13 days) were orally administered PBS (0.2 ml) or α-glucosyl hesperidin (200 mg/kg) on days 0, 1 and 2. Sodium selenite was subcutaneously administered to the rats 4 h after the first oral administration on day 0. Antioxidant levels in the lens and blood were measured on day 6. In vitro, human lens epithelial cells were treated with sodium selenite (10 µM) and/or hesperetin (50 or 100 mM) for 24 h and analyzed for apoptosis markers using sub-G 1 population and Annexin V-FITC/propidium iodide staining and DNA ladder formation. α-glucosyl hesperidin treatment significantly reduced the severity of selenite-induced cataract. The level of antioxidants was significantly reduced in the selenite-treated rats compared with in the controls; however, they were normalized with α-glucosyl hesperidin treatment. In vitro, hesperetin could significantly reduce the number of cells undergoing apoptosis induced by sodium selenite in human lens epithelial cell lines. Overall, oral consumption of α-glucosyl hesperidin could delay the onset of selenite-induced cataract, at least in part by modulating the selenite-induced cell death in lens epithelial cells.

Optimization of Ultrasound-Assisted Extraction via Sonotrode of Phenolic Compounds from Orange By-Products

“…A hesperidin derivate, alpha-glucosyl hesperidin, previously found in the orange peel by several authors [ 62 , 63 , 64 , 65 , 66 ] has been identified at time 8.481 min (peak 25). In addition, a naringin derivate found at peak 34 was assigned to naringin hydrate according with Iglesias-Carres et al [ 55 ] and Fayek et al [ 59 ].…”

Oral consumption of α-glucosyl-hesperidin could prevent lens hardening, which causes presbyopia

“…Besides surgical removal of the cataractous lens and insertion of an artificial lens, there is no effective drug treatment to cure cataracts. It was reported that lens sclerosis and presbyopia are early observed symptoms of age-related nuclear cataract [ 2 ], and we previously reported that oral consumption of G-Hsd delayed the onset of cataract induced by sodium selenite [ 5 ]. So, we hypothesized that G-Hed could prevent lens sclerosis, and here we show that lens elasticity was maintained by G-Hsd treatment under the same experimental conditions ( Fig.…”

“…Therefore, daily intake of antioxidants is a useful approach for the prevention of both presbyopia and cataract formation. In this study, we investigated the effects of hesperetin (Hst), a natural antioxidant, which we have previously reported to have anti-cataract effects in a selenite-induced cataract rat model [ 4 , 5 ]. Hst is the aglycone of hesperidin (Hsd), which is the major flavonoid in citrus fruits.…”

“…On the contrary, α-glucosyl Hsd (G-Hsd), prepared by Yamada et al, has a solubility in water approximately 10,000 times greater than that of Hst and Hsd, with more than three-fold increased bioavailability [ 6 ]. We previously reported that oral intake of G-Hsd ameliorated cataract formation in a rat model [ 5 ]. However, no studies have investigated of the effects of Hst and/or G-Hsd in preventing the onset of presbyopia.…”

Effect of Alpha-Glucosyl-Hesperidin Consumption on Lens Sclerosis and Presbyopia

“…We previously reported that α-glucosyl-hesperidin (G-Hsd) prevents nuclear cataract formation induced by selenite administration [ 4 ]. G-Hsd was synthesized by the glycosylation process with hesperidin (Hsd), which is the major and abundant flavonoid found in citrus fruits.…”