Oral Intake of Collagen Peptide Attenuates Ultraviolet B Irradiation-Induced Skin Dehydration In Vivo by Regulating Hyaluronic Acid Synthesis

Abstract

Collagen peptide (CP) has beneficial effects on functions of the skin, such as skin barrier function and skin elasticity, in vivo. However, there are few studies investigating the mechanism underlying the potential effects of CP in skin epidermal moisturization after ultraviolet B (UVB) irradiation. In this study, we examined whether orally-administered CP affects the loss of skin hydration induced by UVB irradiation in hairless mice. SKH-1 hairless mice were orally administered CP at two doses (500 and 1000 mg/kg) for nine weeks, and the dorsal skin was exposed to UVB. The potential effects of CP were evaluated by measuring the transepidermal water loss (TEWL), skin hydration, wrinkle formation, and hyaluronic acid expression in the dorsal mice skin. We found that oral administration of CP increased skin hydration and decreased wrinkle formation compared to the UVB-irradiated group. Treatment of CP increased the mRNA and protein expression of hyaluronic acid synthases (HAS-1 and -2) concomitant with an increased hyaluronic acid production in skin tissue. The expression of hyaluronidase (HYAL-1 and 2) mRNA was downregulated in the CP-treated group. In addition, the protein expression of skin-hydrating factors, filaggrin and involucrin, was upregulated via oral administration of CP. In summary, these results show that oral administration of CP increases hyaluronic acid levels, which decreases during UVB photoaging. Therefore, we suggest that CP can be used as a nutricosmetic ingredient with potential effects on UVB-induced skin dehydration and moisture loss in addition to wrinkle formation.

Oral Supplementation of Collagen Peptides Improves Skin Hydration by Increasing the Natural Moisturizing Factor Content in the Stratum Corneum: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial

“…Here, we found that in the SC, the level of the NMF constituents, PCA and UCA, significantly increased following the ingestion of CP, and the total amino acids tended to increase. This result is consistent with the result that oral administration of CP promoted filaggrin expression in mice [29]. On the contrary, the CPs, Pro-Hyp and Hyp-Gly, did not induce HA production in keratinocytes in vitro (data not shown).…”

Anti-Photoaging Effects of Four Insect Extracts by Downregulating Matrix Metalloproteinase Expression via Mitogen-Activated Protein Kinase-Dependent Signaling

“…Skin hydration is strongly linked to skin barrier function, and is important for maintaining a healthy skin barrier [25]. UV irradiation increases the TEWL to result in the damage or weakening of the skin barrier function, leading to wrinkle formation [26]. Also, HA and TGF-β are well-known to improve skin hydration reduced by UVB irradiation [27].…”

A Peptide YGDEY from Tilapia Gelatin Hydrolysates Inhibits UVB‐mediated Skin Photoaging by Regulating MMP‐1 and MMP‐9 Expression in HaCaT Cells

“…They can be secreted via keratinocytes under various stimuli such as UVB irradiation, oxidative stress and cytokines and involved in the degradation and synthesis inhibition of collagen fibers in extracellular matrices (ECM) . As an important member in MMPs, MMP‐1 and MMP‐9 co‐degrade collagen, elastin and other structural proteins in ECM , which not only leads to wrinkle formation, sagging skin, but also exacerbate trans epidermal water loss and reduces skin hydration . Type I procollagen is the main structural component of ECM, and MMP‐1 specifically degrades Type I procollagen during photoaging .…”