Oral inoculation of ultraviolet-irradiated Eimeria species oocysts protects chickens against coccidiosis

El‐Ashram, Saeed; Aboelhadid, Shawky M.; Gadelhaq, Sahar M.; Arafa, Waleed M.; Abdel‐Razik, Abdel‐Razik H.; Abohamra, Salama; Abd-Elaziz, Khaled

doi:10.1007/s00436-019-06455-y

Cited by 12 publications

(4 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oocysts were sporulated in a 2.5% (w/v) potassium dichromate solution at 28 ˚ C for three days before being washed three times with normal saline. After assessing the total number of oocysts using the McMaster procedure, the sporulated oocysts were diluted to 5 × 10 5 oocysts/mL in normal saline [ 2 , 3 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

Efficacy of liver free and Chitosan against Eimeria tenella in chickens

Yu,

Alouffi,

Al-Olayan

et al. 2024

BMC Vet Res

View full text Add to dashboard Cite

Eimeria spp. are the pathogen that causes coccidiosis, a significant disease that affects intensively reared livestock, especially poultry. Anticoccidial feed additives, chemicals, and ionophores have routinely been employed to reduce Eimeria infections in broiler production. Therefore, the shift to antibiotic-free and organic farming necessitates novel coccidiosis preventive strategies. The present study evaluated the effects of potential feed additives, liver free and chitosan, against Eimeria tenella infection in White Leghorn broiler female chickens. One hundred sixty-five 1-day-old White Leghorn broiler female chicks were divided into 11 groups (15 female chicks per group), including the positive control group (G1), the negative control group (G2), a chitosan-treated group (G3), a chitosan-treated-infected group (G4), the liver free-treated group (G5), the liver free-treated-infected group (G6), the liver free-and-chitosan-treated group (G7), the liver free-and-chitosan-infected group (G8), the therapeutic liver free-and-chitosan-treated-infected group (G9), the sulfaquinoxaline-treated group (G10), and the sulfaquinoxaline-treated-infected group (G11). Chitosan was fed to the chicks in G3 and G4 as a preventative measure at a dose of 250 mg/kg. The G5 and G6 groups received 1.5 mg/kg of Liverfree. The G7 and G8 groups received chitosan and Liverfree. The G10 and G11 groups were administered 2 g/L of sulfaquinoxaline. From the moment the chicks arrived at Foshan University (one-day-old chicks) until the completion of the experiment, all medications were given to them as a preventative measure. G8 did; however, receive chitosan and liver free as therapeutic supplements at 7 dpi. The current study showed that the combination of liver free and chitosan can achieve better prophylactic and therapeutic effects than either alone. In E. tenella challenged chickens, G8 and G9 chickens showed reduced oocyst shedding and lesion score, improved growth performance (body weight, body weight gain, feed intake, feed conversion ratio, and mortality rate), and cecal histology. The current study demonstrates that combining liver free and chitosan has superior preventive and therapeutic benefits than either alone, and they could also be used as alternative anticoccidial agents.

show abstract

Section: Methodsmentioning

confidence: 99%

Efficacy of liver free and Chitosan against Eimeria tenella in chickens

Yu,

Alouffi,

Al-Olayan

et al. 2024

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…First, cecal coccidiosis can be controlled by reducing the intake of sporulated oocysts ( 21 , 146 ). These sporocysts can be controlled by in vitro sporicidal substances, which may kill the sporocysts or stop the sporulation of oocysts ( 147 ).…”

Section: Methodsmentioning

confidence: 99%

Botanicals: A promising approach for controlling cecal coccidiosis in poultry

Saeed

Alkheraije

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Avian species have long struggled with the problem of coccidiosis, a disease that affects various parts of the intestine, including the anterior gut, midgut, and hindgut. Among different types of coccidiosis, cecal coccidiosis is particularly dangerous to avian species. Chickens and turkeys are commercial flocks; thus, their parasites have remained critical due to their economic importance. High rates of mortality and morbidity are observed in both chickens and turkeys due to cecal coccidiosis. Coccidiostats and coccidiocidal chemicals have traditionally been added to feed and water to control coccidiosis. However, after the EU banned their use because of issues of resistance and public health, alternative methods are being explored. Vaccines are also being used, but their efficacy and cost-effectiveness remain as challenges. Researchers are attempting to find alternatives, and among the alternatives, botanicals are a promising choice. Botanicals contain multiple active compounds such as phenolics, saponins, terpenes, sulfur compounds, etc., which can kill sporozoites and oocysts and stop the replication of Eimeria. These botanicals are primarily used as anticoccidials due to their antioxidant and immunomodulatory activities. Because of the medicinal properties of botanicals, some commercial products have also been developed. However, further research is needed to confirm their pharmacological effects, mechanisms of action, and methods of concentrated preparation. In this review, an attempt has been made to summarize the plants that have the potential to act as anticoccidials and to explain the mode of action of different compounds found within them.

show abstract

“…Although attenuated vaccines are safe, the cost of their productions is high due to the reduced fecundity of precocious parasite (Williams 2002), and 2-3 cycles of Eimeria are necessary for maximum acquired immunity (Blake et al 2017). Treatment with UV-irradiated oocysts significantly reduced oocyst shedding and maintained cecal mucosal integrity; moreover, the body weight was higher in chickens inoculated with irradiated oocysts than their non-irradiated counterparts (El-Ashram et al 2019).…”

Section: Immunological and Genetic Manipulations 31 Vaccinesmentioning

confidence: 99%

Avian Coccidiosis: Recent Advances in Alternative Control Strategies and Vaccine Development

Khater¹

2020

Agrobiol. Records

View full text Add to dashboard Cite

Coccidiosis induces huge economic losses to poultry production. Its control through anticoccidial live vaccines and drugs has been very successful with some limitations because of the cost of production of live vaccines, drug resistance, and residues representing public health concerns. Consequently, there is a crucial need for drug-free production of foods. Useful strategies include environmental, immunological, and genetic approaches; feed additives are recent attitudes involving probiotics, synbiotics, organic acids, phytobiotics as essential oils, antioxidants, and nanobiotics (nanoparticles). A combination of such additives is a recent useful trend. Transgenic Eimeria parasite could fill in the gap in the control of chicken coccidiosis as an efficient anticoccidial vaccine with improved protective efficacy using multiple vaccine antigens. Alternatives justify further studies as therapeutic or prophylactic anticoccidial agents. Full biological and toxicology profiles are crucial for the promising materials which deserve to be applied on a larger scale.

show abstract

Oral inoculation of ultraviolet-irradiated Eimeria species oocysts protects chickens against coccidiosis

Cited by 12 publications

References 64 publications

Efficacy of liver free and Chitosan against Eimeria tenella in chickens

Efficacy of liver free and Chitosan against Eimeria tenella in chickens

Botanicals: A promising approach for controlling cecal coccidiosis in poultry

Avian Coccidiosis: Recent Advances in Alternative Control Strategies and Vaccine Development

Contact Info

Product

Resources

About