Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats

Abstract

We investigated the effects of the oral administration of lophenol (Lo) and cycloartanol (Cy), two kinds of antidiabetic phytosterol isolated from Aloe vera , on glucose and lipid metabolism in Zucker diabetic fatty (ZDF) rats. We demonstrated that the administrations of Lo and Cy suppressed random and fasting glucose levels and reduced visceral fat weights significantly. It was also observed that treatments with Lo and Cy decreased serum and hepatic lipid concentrations (triglyceride, nonesterified fatty acid, and total cholesterol). Additionally, Lo and Cy treatments resulted in a tendency for reduction in serum monocyte chemotactic protein-1 (MCP-1) level and an elevation in serum adiponectin level. Furthermore, the expression levels of hepatic genes encoding gluconeogenic enzymes (G6 Pase, PEPCK), lipogenic enzymes (ACC, FAS), and SREBP-1 were decreased significantly by the administrations of aloe sterols. In contrast, Lo and Cy administration increased mRNA levels of glycolysis enzyme (GK) in the liver. It was also observed that the hepatic β-oxidation enzymes (ACO, CPT1) and PPARα expressions tended to increase in the livers of the Lo- and Cy-treated rats compared with those in ZDF-control rats. We therefore conclude that orally ingested aloe sterols altered the expressions of genes related to glucose and lipid metabolism, and ameliorated obesity-associated metabolic disorders in ZDF rats. These findings suggest that aloe sterols could be beneficial in preventing and improving metabolic disorders with obesity and diabetes in rats.

Dietary Aloe Vera Gel Powder and Extract Inhibit Azoxymethane-induced Colorectal Aberrant Crypt Foci in Mice Fed a High-fat Diet

“…Lophenol and cycloartanol, two kinds of Aloe-sterols, reduced visceral fat accumulation and improved hyperlipidemia and hyperglycemia in ZDF rats fed a high-fat diet (Misawa et al, 2008). Furthermore, these of Aloe-sterols altered the expression levels of genes related to glucose and lipid metabolism, and ameliorated obesity-associated metabolic disorders DOI:http://dx.doi.org/10.7314/APJCP.2015.16.2.683 Dietary Aloe Vera Inhibits Azoxymethane-Induced Colorectal Aberrant Crypt Foci in Mice Fed a High-Fat Diet. in ZDF rats given a high-fat diet (Misawa et al, 2012b). In the present study, ACF percentage inhibition was smaller in the H-AVGE group than in the 3% AVGP group.…”

“…Therefore, the mRNA expression levels of the two enzymes in the 3% AVGP group were estimated to be similar to those in the H-AVGE group. Misawa et al (2012b) speculated that Aloe-sterols in bioactive molecules containing Aloe vera were the main active ingredients with anti-obesity effects in a high-fat diet-induced obesity rat model. Recent epidemiological studies have also reported that obesity may be a major risk factor in many cancers, especially cancers of the colon and breast (Sung et al, 2011).…”

Administration ofAloe arborescenshomogenate to cattle: interaction with rumen fermentation and gut absorption of aloin

“…whole plant or from some of its components is scanty and only a recent cause of interest (Gutterman & Chauser-Volfson 2000;Beppu et al 2006;Shin et al 2011;Misawa et al 2012). No information is available on the fate of active components from Aloe spp.…”

Immune Modulation of Aloe vera: Acemannan and Gut Microbiota Modulator

“…Orally ingested aloe sterols altered the expressions of genes related to glucose and lipid metabolism, and ameliorated obesity-associated metabolic disorders in ZDF rats. These findings by Misawa E. group suggest that aloe sterols could be beneficial in preventing and improving metabolic disorders with obesity and diabetes in rats [46] .…”