Oral infection of mice with Salmonella entericaserovar Typhimurium causes meningitis and infection of the brain

Wickham, Mark; Brown, Nathaniel Francis; Provias, John; Finlay, B. Brett; Coombes, Brian K.

doi:10.1186/1471-2334-7-65

Cited by 38 publications

(37 citation statements)

References 22 publications

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“…Significant progress has been made in understanding the molecular interactions between the BBB and microbial pathogens with the availability of in vitro tissue culture models of human BMEC (hBMEC) (Stins et al 1994;Weksler et al 2005) and in vivo animal models (Smith et al 1973;Doran et al 2002;Wickham et al 2007;Uchiyama et al 2009;Sheen et al 2010). Microbial interactions with the BBB may involve crossing of BMEC in a vacuole (transcytosis), through the intercellular junctional spaces ( paracytosis), or while inside a host cell (e.g., infected phagocyte) using it as a vehicle to cross the barrier (Trojan horse) (Kim 2008(Kim , 2010 (Fig.…”

Section: Pathogen Invasion Of the Bbbmentioning

confidence: 99%

Concepts and Mechanisms: Crossing Host Barriers

Doran

Banerjee

Disson

et al. 2013

Cold Spring Harbor Perspectives in Medicine

111

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Section: Pathogen Invasion Of the Bbbmentioning

confidence: 99%

Concepts and Mechanisms: Crossing Host Barriers

Doran

Banerjee

Disson

et al. 2013

Cold Spring Harbor Perspectives in Medicine

111

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“…Human infection with Salmonella Typhi can be modeled in the laboratory through the infection of susceptible mice with Salmonella Typhimurium (34). In this model, systemic infection ensues and Salmonella can infect multiple organs, such as the gastrointestinal tract, brain, liver, spleen, and gallbladder (19,42). Many aspects of host colonization in this murine model are reminiscent of human Salmonella Typhi infection.…”

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confidence: 99%

Repression of Salmonella entericaphoPExpression by Small Molecules from Physiological Bile

et al. 2012

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Infection with Salmonella enterica serovar Typhi in humans causes the life-threatening disease typhoid fever. In the laboratory, typhoid fever can be modeled through the inoculation of susceptible mice with Salmonella enterica serovar Typhimurium. Using this murine model, we previously characterized the interactions between Salmonella Typhimurium and host cells in the gallbladder and showed that this pathogen can successfully invade gallbladder epithelial cells and proliferate. Additionally, we showed that Salmonella Typhimurium can use bile phospholipids to grow at high rates. These abilities are likely important for quick colonization of the gallbladder during typhoid fever and further pathogen dissemination through fecal shedding. To further characterize the interactions between Salmonella and the gallbladder environment, we compared the transcriptomes of Salmonella cultures grown in LB broth or physiological murine bile. Our data showed that many genes involved in bacterial central metabolism are affected by bile, with the citric acid cycle being repressed and alternative respiratory systems being activated. Additionally, our study revealed a new aspect of Salmonella interactions with bile through the identification of the global regulator phoP as a bile-responsive gene. Repression of phoP expression could also be achieved using physiological, but not commercial, bovine bile. The biological activity does not involve PhoPQ sensing of a bile component and is not caused by bile acids, the most abundant organic components of bile. Bioactivity-guided purification allowed the identification of a subset of small molecules from bile that can elicit full activity; however, a single compound with phoP inhibitory activity could not be isolated, suggesting that multiple molecules may act in synergy to achieve this effect. Due to the critical role of phoP in Salmonella virulence, further studies in this area will likely reveal aspects of the interaction between Salmonella and bile that are relevant to disease.

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“…Recently, Wickham et al (30) reported that oral infection of mice with wild-type S. enterica serovar Typhimurium strains can cause meningoencephalitis. We have confirmed these observations and determined that such infections also arise after inoculation with wild-type strains by the i.n.…”

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confidence: 99%

Presence of Wild-Type and Attenuated Salmonella enterica Strains in Brain Tissues following Inoculation of Mice by Different Routes

Bollen

Gunn

et al. 2008

Infect Immun

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Oral infection of mice with Salmonella entericaserovar Typhimurium causes meningitis and infection of the brain

Abstract: Background: Salmonella meningitis is a rare and serious infection of the central nervous system following acute Salmonella enterica sepsis. For this pathogen, no appropriate model has been reported in which to examine infection kinetics and natural dissemination to the brain.

Cited by 38 publications

References 22 publications

Concepts and Mechanisms: Crossing Host Barriers

Concepts and Mechanisms: Crossing Host Barriers

Repression of Salmonella entericaphoPExpression by Small Molecules from Physiological Bile

Presence of Wild-Type and Attenuated Salmonella enterica Strains in Brain Tissues following Inoculation of Mice by Different Routes

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