Oral immunoglobulin treatment in Campylobacter jejuni enteritis

Hammarström, Viera; Smith, C. I. Edvard; Hammarström, Lennart; Heaton, Paul Anthony

doi:10.1016/0140-6736(93)91136-a

Cited by 43 publications

(30 citation statements)

References 9 publications

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“…Roos et al (1995) demonstrated that as much as 19% of ingested IgG and IgM remain immunologically active in the intestine of adult humans. Orally administered human Ig has been shown to reduce the subclinical symptoms of necrotizing enterocolitis (Eibl et al, 1988), whereas Ig has also been used therapeutically against Campylobacter jejuni (Hammarströ m et al, 1993) and Clostridium difficile (Tjellstrom et al, 1993), a major cause of diarrhoea in immunocompromised patients as well as chronic diarrhoea of unknown aetiology in normal infants . The successful treatment of Helicobacter pylori, rotavirus and cryptosporidial infection using bovine Ig has also been reported .…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with ovine serum immunoglobulin attenuates acute effects on growth, organ weights, gut morphology and intestinal mucin production in the growing rat challenged with Salmonella enteritidis

Balan

Han

Rutherfurd

et al. 2011

Animal

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The aim was to determine the effect of orally administered ovine serum immunoglobulin (Ig) on growth performance, organ weight, gut morphology and mucin production in the Salmonella enteritidis -gavaged growing rat. Four groups consisted of non-gavaged rats fed a casein-based control basal diet (BD) and three groups of rats gavaged with 1 3 10 7 CFU S. enteritidis and fed a casein-based diet, a diet containing freeze-dried ovine Ig (FDOI) or a casein-based diet containing inactivated ovine Ig (IOI). The rats were randomly allocated to one of the four groups (n 5 15/group) and received their respective diets for an 18-day experimental study. Gavaging took place on day 15. Average daily gain and body gain : feed ratio (post-gavage, 3 days) were significantly (P , 0.05) higher for the Salmonella-challenged rats fed the FDOI diet compared to those fed the BD and IOI diets. At the end of the study, the small intestine and colon were significantly (P , 0.05) heavier for the gavaged rats fed the FDOI diet compared to the gavaged rats fed either the BD or IOI diet. Moreover, the relative weights of the caecum, liver and spleen of the gavaged rats fed the BD or IOI diet were significantly (P , 0.05) heavier compared to the gavaged rats fed the FDOI diet. Generally, the gavaged rats fed the FDOI diet had significantly (P , 0.05) higher goblet cell counts and luminal mucin protein contents than the gavaged rats fed either the BD or IOI diet and had a more functional gut morphology. Overall, the FDOI fraction prevented the acute effects of S. enteritidis.

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Section: Introductionmentioning

confidence: 99%

Dietary supplementation with ovine serum immunoglobulin attenuates acute effects on growth, organ weights, gut morphology and intestinal mucin production in the growing rat challenged with Salmonella enteritidis

Balan

Han

Rutherfurd

et al. 2011

Animal

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“…Several studies carried out in production animals at weaning (Kats et al, 1994;Torrallardona et al, 2003;Pierce et al, 2005) have demonstrated beneficial effects from the ingestion of spray-dried animal plasma (SDAP) containing Ig, such as increased intestinal tissue growth rate, increased feed intake and higher body weight (BW) gain. Furthermore, the beneficial effects of feeding SDAP are more pronounced with higher pathogen challenge, as when pigs were housed in an on-farm conventional nursery, which was exposed to a greater load of subclinical pathogenic organisms, the animals exhibited an enhanced growth rate and feed intake compared to those reared in the off-site experimental (cleaner) settings (Coffey and Cromwell, 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Human Ig has been used as a prophylactic treatment for children born prematurely with necrotizing enterocolitis (Eibl et al, 1988). To date, Ig has been used therapeutically for intervention in Campylobacter jejuni (Hammarstrom et al, 1993), Clostridium difficile (Tjellstrom et al, 1993), Helicobacter pylori, rotavirus and cryptosporidial infections.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of ovine serum immunoglobulin in the growing rat

Balan

Han

Rutherfurd-Markwick

et al. 2010

Animal

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This study aimed to determine whether orally administered ovine serum immunoglobulin (Ig) modulates aspects of immunity such as phagocytosis, lymphocyte proliferation, cytokine production, intestinal and plasma Ig concentrations in growing rats. Forty-five male Sprague-Dawley rats (n 5 15/group) were used in the 21-day study, and fed a basal control diet (BD; no Ig) or two test diets: freeze-dried ovine Ig (FDOI) and inactivated ovine Ig (IOI). Phagocytic activity of peripheral blood leukocytes and lymphocyte proliferation in the presence of the mitogen concanavalin A (ConA) was greater ( P , 0.05) for the FDOI-fed rats than for the BD-and IOI-fed groups. ConA-stimulated and unstimulated spleen cell culture produced higher ( P , 0.05) interferon-g and interleukin-4, respectively, from rats fed the FDOI than rats fed the BD diet. In the jejunum, ileum and plasma, rats fed FDOI produced higher ( P , 0.05) concentrations of secretory IgA (sIgA) than rats fed IOI or BD. Rats fed the FDOI diet had greater jejunal ( P 5 0.037) and lower plasma ( P 5 0.025) rat IgG concentrations than rats fed either BD or IOI. In conclusion, an ovine Ig fraction selectively modulated various indices of immune function.

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“…During transport from the basolateral to the apical membrane of the epithelial cell, the extracellular portion of the pIgR is cleaved, resulting in the external secretion of secretory IgA (sIgA) consisting of a secretory component (SC) bound to an IgA dimer (Kraehenbuhl and Neutra, 1992). The potential of orally and nasally delivered IgA for protection of mucosal surfaces against viral and bacterial pathogens has been recently demonstrated (Michetti et al, 1992;Czinn et al, 1993;Hammarstrom et al, 1993;Thomas et al, 1993;Mazanec et al, 1995), using hybridoma cells as a convenient source of protective IgA. In order to conduct studies aiming at comparing the function of IgA with slgA, SC has, however, to be produced in heterologous expression systems in milligram quantities and in the native conformation prior to reconstitution with dimeric IgA (Lullau et al, 1996).…”

mentioning

confidence: 99%

Cellular Processing Limits the Heterologous Expression of Secretory Component in Mammalian Cells

Cottet¹,

Corthésy²

1997

European Journal of Biochemistry

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Recombinant vaccinia-virus-based expression systems are very popular for the overproduction of proteins in mammalian cell lines. Both the double virus T7/vaccinia hybrid system and the single recombinant strategy based on the p l l K late promoter were evaluated for their ability to govern expression and secretion of recombinant human secretory component (SC), a glycoprotein associated with IgA in mucosal secretions. We report here that, while the T7 promoter is transcriptionally 3.4-fold more active than the p l l K promoter, no difference in levels of secreted recombinant human SC is observed using either vaccinia system to infect CV-1 cells. High transcription, and thus translation levels, lead to saturation of early processing steps involved in protein export. Both systems exhibit transient accumulation of comparable amount of recombinant human SC in the endoplasmic reticulum andor the cis Golgi network, as demonstrated by immunofluorescence and endoglycosidase H (EndoH) sensitivities. Exposure of infected cells to tunicamycin results in similar inhibition of recombinant human SC export, further arguing that N-linked glycosylation is necessary for proper folding and subsequent secretion. Moreover, pulse-chase experiments indicate that newly synthesized recombinant human SC is not completely processed in a mature glycoprotein and that a portion of overexpressed SC might be degraded before it can be secreted. Recombinant human SC behaves identically to native SC in terms of kinetics of secretion and IgAbinding capacity.Our results indicate that optimization of expression systems should not only rely on the design of effective vectors, but also on the identification and clearance of the cellular bottlenecks associated with maturation of the secreted proteins.

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Oral immunoglobulin treatment in Campylobacter jejuni enteritis

Cited by 43 publications

References 9 publications

Dietary supplementation with ovine serum immunoglobulin attenuates acute effects on growth, organ weights, gut morphology and intestinal mucin production in the growing rat challenged with Salmonella enteritidis

Dietary supplementation with ovine serum immunoglobulin attenuates acute effects on growth, organ weights, gut morphology and intestinal mucin production in the growing rat challenged with Salmonella enteritidis

Immunomodulatory effects of ovine serum immunoglobulin in the growing rat

Cellular Processing Limits the Heterologous Expression of Secretory Component in Mammalian Cells

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