Oral immunogenicity of the inactivated Vibrio cholerae whole-cell vaccine encapsulated in biodegradable microparticles

Yeh, Ming–Kung; Liu, Yu-Tien; Chen, Jiin-Long; Chiang, Chiao–Hsi

doi:10.1016/s0168-3659(02)00140-2

Cited by 34 publications

(12 citation statements)

References 18 publications

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“…The approach of multiple emulsion (water-in oil-in water; w/o/w) combined with a solvent evaporation method is widely used to encapsulate drugs in PLGA or PLA matrices for preparing drug-loaded microparticles (Coombes et al 1998, Yeh et al 2002b). By using PLGA or PLA microparticle delivery systems, carried drugs are either adsorbed on the surface region or encapsulated in the whole polymeric matrix of microparticles (Herrmann andBodmeier 1995, Yeh et al 2002a, c).…”

Section: Introductionmentioning

confidence: 99%

The mechanism of surface-indented protein-loaded PLGA microparticle formation: the effects of salt (NaCl) on the solidification process

Chen

Yeh

Chiang

2004

Journal of Microencapsulation

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The purpose of this study was to evaluate ovalbumin (OVA) leakage pathways and to explore the mechanism of the surface-indented microparticle formation in the preparation of OVA-loaded microparticles. OVA-loaded poly (D,L-lacticco-glycolic acid) (PLGA) microparticles were prepared by a water-in oil-in water (w/o/w) solvent evaporation method associated with varied NaCl (NaCl) concentrations and adjusted with urea at 1240 mOsm kg À1 in the external aqueous phase. To evaluate dichloromethane (DCM)-related OVA leakage, three stirring rates, 600, 800, 1000 rpm at 25 C were carried out during the solvent evaporation stage. Both DCM and OVA levels in the external phase medium and total dispersion were sampled and measured. The time course of particle characteristics was evaluated by microscopy or SEM photography. The surface adsorptive capacities of the prepared microparticles were measured by using bovine serum albumin conjugated with fluorescein isothiocyanate (FITC-BSA). The findings were that the DCM-related OVA leakage accounted for $34% of the total leakage. By combining NaCl in the external phase, a faster solidifying crust-like structure was formed as a barrier to remarkably reduce OVA loss and improve OVA content from 40.1 to 72.8 mg mg À1 . The yield and OVA content for formulations containing NaCl were much improved by the ionic effect, in addition to the osmotic effect. The total entrapment efficiency was also highly increased from 43 to 72%. The formations of the crust-like surface structure of the microparticle were affected by entrapped drugs, salt content in the external phase and aqueous volume in the inner phase. A scheme was proposed to interpret the formation mechanism of the surfaceindented microparticles. In comparison to the surface-smooth microparticles, the surface adsorptive capacities of the surface-indented microparticles were highly improved from 26.6 to 87.0%, determined by the adsorption of FITC-BSA.

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Section: Introductionmentioning

confidence: 99%

The mechanism of surface-indented protein-loaded PLGA microparticle formation: the effects of salt (NaCl) on the solidification process

Chen

Yeh

Chiang

2004

Journal of Microencapsulation

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“…45 Compared with the administration of a VC solution, significantly higher serum titers of Vibriospecific immunoglobulin (Ig) G and IgM antibodies were elicited in mice immunized with VC-loaded microspheres. These VC-loaded microspheres were also prepared in different combinations: 50:50 PLG, 75:25 PLG, and PLA/poly (ethylene glycol) (PEG) blended microspheres.…”

Section: Cholera Vaccinementioning

confidence: 99%

Biodegradable polymeric microsphere-based vaccines and their applications in infectious diseases

Lin

Yang

et al. 2015

Human Vaccines & Immunotherapeutics

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“…The HS distribution in the microparticles (i.e. the amount of HS associated with either the surface or the core of loaded microparticles) was estimated by a modification of previously described methods (Coombes et al 1998, Yeh et al 2002. Briefly, 10 mg of each formulation of microparticles were dispersed in 1 ml of water and magnetically stirred for 1 h at room temperature.…”

Section: Physico-chemical Characterizationmentioning

confidence: 99%

Optimization of the entrapment of bacterial cell envelope extracts into microparticles for vaccine delivery

Estevan

Gamazo

González‐Gaitano

et al. 2006

Journal of Microencapsulation

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The encapsulation of a Brucella ovis extract (HS) in microparticles has been proved effective against experimental infections in mice. This work describes different strategies to increase the HS loading and prepare large batches as necessary to test this vaccine in ovine. The mixture of HS with beta-cyclodextrin was optimized in order to increase the HS loading in microparticles. On the other hand, TROMS ('Total Recirculation One-Machine System') led microparticles with a more homogeneous size than the laboratory or standard procedure. Moreover, the initial burst release of HS from the standard microparticles was higher than for the TROMS ones. In fact, standard microparticles displayed a higher amount of adsorbed HS. On the contrary, both preparative methods were found effective to preserve the integrity and anti-genicity of the loaded HS. In summary, beta-CD can be used to increase the loading of large hydrophobic materials and TROMS is a valid large production of antigen-loaded microparticles.

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Oral immunogenicity of the inactivated Vibrio cholerae whole-cell vaccine encapsulated in biodegradable microparticles

Cited by 34 publications

References 18 publications

The mechanism of surface-indented protein-loaded PLGA microparticle formation: the effects of salt (NaCl) on the solidification process

The mechanism of surface-indented protein-loaded PLGA microparticle formation: the effects of salt (NaCl) on the solidification process

Biodegradable polymeric microsphere-based vaccines and their applications in infectious diseases

Optimization of the entrapment of bacterial cell envelope extracts into microparticles for vaccine delivery

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