Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit

Matsumoto, Yasunobu; Suzuki, Shigenori; Nozoye, Tomoko; Yamakawa, Takashi; Takashima, Yasuhiro; Arakawa, Takeshi; Tsuji, Naotoshi; Takaiwa, Fumio; Hayashi, Yoshihiro

doi:10.1007/s11248-008-9205-4

Cited by 43 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, edible vaccines produced in papaya and corn seed induced protection against porcine-cysticercosis (70-90%) and porcine-transmissible gastroenteritis virus (50%) [76,77]. Even more, edible vaccines can include adjuvants as it was the case for As16-an antigen protective against the roundworm Ascaris suum fused with CTB in transgenic rice seeds, resulting in an antibody response [78].…”

Section: Plant-based Veterinary Vaccinementioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

View full text Add to dashboard Cite

Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

show abstract

Section: Plant-based Veterinary Vaccinementioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

View full text Add to dashboard Cite

show abstract

“…Ascaris suum infects pigs (but not humans), resulting in a decrease in daily weight gain and an increase in feed-to-gain ratio, resulting in economic losses (Stewart and Hale 1988). Matsumoto et al (2009) produced a 16-kDa protein of A. suum (As16) fused with CTB in transgenic rice seeds, and demonstrated that oral immunization with the chimeric protein together with mucosal CT adjuvant induced As16-specific serum IgG antibodies and reduced the number of larvae in the lungs after infection with A. suum embryonated eggs. Human roundworm, Ascaris lumbricoides, has a protein identical to A. suum As16 (Tsuji et al 2003).…”

Section: Matsumoto Et Al (2009) Reported a New Rice-based Vaccine Agmentioning

confidence: 99%

Novel Transgenic Rice-Based Vaccines

Azegami

Itoh

Kiyono

et al. 2014

Arch. Immunol. Ther. Exp.

View full text Add to dashboard Cite

Oral vaccination can induce both systemic and mucosal antigen-specific immune responses. To control rampant mucosal infectious diseases, the development of new effective oral vaccines is needed. Plant-based vaccines are new candidates for oral vaccines, and have some advantages over the traditional vaccines in cost, safety, and scalability. Rice seeds are attractive for vaccine production because of their stability and resistance to digestion in the stomach. The efficacy of some rice-based vaccines for infectious, autoimmune, and other diseases has been already demonstrated in animal models. We reported the efficacy in mice, safety, and stability of a rice-based cholera toxin B subunit vaccine called MucoRice-CTB. To advance MucoRice-CTB for use in humans, we also examined its efficacy and safety in primates. The potential of transgenic rice production as a new mucosal vaccine delivery system is reviewed from the perspective of future development of effective oral vaccines.

show abstract

“…Tomato was tested as an edible vaccine against malaria (Chowdhury and Bagasra 2007 ), lettuce against the E. coli heat-labile toxin B subunit (Martínez-González et al 2011 ), rice against the roundworm Ascaris suum (Matsumoto et al 2009 ). Dorokhov et al ( 2007 ) A complete review about plant-made vaccines was published by Awale et al ( 2012 ).…”

Section: Vaccinesmentioning

confidence: 99%

Molecular Farming in Plants

Álvarez

2014

Plant Biotechnology for Health

View full text Add to dashboard Cite

Molecular farming can generally be defi ned as the production of molecules (proteins, fatty acids) for the pharmaceutical and chemical industries in transgenic organisms (plants, animals, etc.).Molecular farming in plants has advantageous aspects as biosecurity, they do not bear pathogens for humans or animals, and they do not produce toxins. Also, plant protein synthetic machinery is able to produce complex glycosylated proteins as they have a glycosylation pattern with slight difference respect of that of mammals, and can perform foldings.When displayed in in vitro conditions, molecular farming have the characteristic advantages of that type of culture, mainly the capacity of working under Good Manufactory practices as is required by the pharmaceutical industry. The critical aspects of plants for molecular farming are the different glycosylation patterns respect to mammals, the duration of the productive process, and the relatively low yields.The approval by the FDA of the fi rst medicine to be used in humans, taliglucerase alfa (ELELYSO®), has give an impulse to this technology but there are some drawbacks to be addressed, particularly related to low yields and regulatory aspects.

show abstract

Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit

Cited by 43 publications

References 36 publications

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Novel Transgenic Rice-Based Vaccines

Molecular Farming in Plants

Contact Info

Product

Resources

About