Oral immunization using HgbA in a recombinant chancroid vaccine delivered by attenuated Salmonella typhimurium SL3261 in the temperature-dependent rabbit model

Production of Monoclonal Antibodies against Common Epitopes of Salmonella by Oral Immunization of Laboratory Animals

“…Similar experiments lead to the production of immunoglobulins M, G, and we have results in the production of immunoglobulins A [7][8][9]. Oral (intragastric) immunization of experimental animals to produce antibodies other than IgA class [9,10] is still rarely performed.…”

“…To demonstrate the immunomodal and polymeric IgA forms, the purified MAbs of each hybridoma clone were separated by SDS-PAGE under reducing and non-reducing conditions and analyzed by immunoblotting with a secondary antibody peroxidaseconjugated a-chain murine-specific antibody [9,10].…”

Antigen Delivery System II

“…This was confirmed by Rizos et al (2003), using Heliocobacter pylori UreA epitopes fused to AIDA-1 for surface display and presentation. Breau et al (2012) encoded the autotransporter HgbA in the pTetCnirB vector for use in S. Typhimurium vaccine strains to enable surface antigen display. Isoda et al (2007) made a C-terminal fusion of the Porphyomonas gingivalis hemagglutinin (HagB) to a Lpp-OmpA fusion for display on the surface of attenuated S. Typhimurium.…”

Salmonella Vaccines: Conduits for Protective Antigens

“…This group further demonstrated that the higher the level of Ag synthesis (which correlated with decreased fitness for the RASV), the more rapid was the induction of immune responses, especially mucosal responses (136). There is much evidence that secretion (119) or surface display of protective Ags on RASVs and other recombinant attenuated bacterial vaccines results in higher immune responses and protective immunity (133,134,(137)(138)(139)(140)(141)(142)(143)(144)(145). Sizemore et al (146) determined that a DphoPQ-attenuated S. Typhimurium strain induced much higher Ab titers against Yersinia pestis Ags F1 and LcrV when these Ags were retained in the cytoplasm rather than when they were secreted.…”

“…In some cases, the ability to display protective Ags on the surface of the RASVs may invoke improved immune responses. Several investigators have reported using fusions of protective Ags to autotransporters, such as AIDA-1 (133) and HgbA (134), for surface display and presentation. Other investigators have used fusions of Ags to Salmonella outer membrane proteins (135), but such fusions have sometimes proven to be toxic to the RASV, necessitating modifications that led to decreased Ag synthesis but still induced much higher serum Abs (IgG and IgA) and higher vaginal IgA to the Porphyromonas gingivalis HagB Ag than were obtained using an isogenic vaccine strain in which HagB remained in the cytoplasm (135).…”