Oral Immunization of Human with transgenic lettuce Expressing Hepatitis B Surface Antigen

Kapusta, Józef; Modelska, A.; Pniewski, Tomasz; Figlerowicz, Magdalena; Jankowski, Kacper; Lisowa, O.; Płucienniczak, Andrzej; Koprowski, Hilary; Legocki, Andrzej B.

doi:10.1007/978-1-4615-0685-0_41

Cited by 60 publications

(40 citation statements)

References 4 publications

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“…1c) and longer intervals between antigen administration on immunogenicity presented here (Fig. 2) is consistent with the previous observation that the humoral response was higher and reached the C10 mIU/ml protective level in human adult volunteers fed on days 1 and 60 (Kapusta et al 1999) than in those fed on days 1, 7, and 30 (Kapusta et al 2001) with 1-2 lg/person plant-associated HBsAg. No humoral response was observed when mice were fed on seven consecutive days with plant-based HBsAg in a total dose of 1 lg (data not shown), which also suggests that multiple and high-dose antigen oral administration is conducive to tolerance rather than immunity.…”

Section: Discussionsupporting

confidence: 81%

“…Many attempts have also been made in the last two decades to develop plant-based vaccines against HBV. Orally administered plant-associated HBsAg was shown to elicit a humoral response in mice (Joung et al 2004;Kapusta et al 1999;Kong et al 2001;Richter et al 2000) and humans (Kapusta et al 1999;Kapusta et al 2001;Mason et al 2003;Thanavala et al 2005). Studies on prototype oral vaccines were predominantly based on multiple administration of relatively high, i.e.…”

Section: Introductionmentioning

confidence: 99%

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Nanogram Doses of Alum-Adjuvanted HBs Antigen Induce Humoral Immune Response in Mice When Orally Administered

Kapusta

Pniewski

Wojciechowicz³

et al. 2010

Arch. Immunol. Ther. Exp.

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Mucosal immunity elicited by plant-based and other orally administered vaccines can serve as the first line of defense against most pathogens infecting through mucosal surfaces, but it is also considered for systemic immunity against blood-borne diseases such as hepatitis B (HB). Previous oral immunization trials based on multiple administration of high doses of HBs antigen elicited an immune response; however, a reproducible and long-lasting immunization protocol was difficult to design. The objective of this study was to evaluate the effect of dose and timing of orally delivered alum-adsorbed antigen on the magnitude of the anti-HBs humoral response. Mice were immunized orally by gavage intubation or parenterally by intramuscular injection three times, once every 2 weeks, with doses of 5, 50, or 500 ng alum-adjuvanted HBsAg. A low dose (10 ng) of HBsAg was orally administered three times in different time intervals: 2, 4, 6, and 8 weeks. The three consecutive 5-ng oral doses of the antigen induced immune response at the protective level (>or=10 mIU/ml), significantly higher than the reaction elicited by three 50 or 500 ng doses. In contrast, intramuscular delivery of these doses did not differ significantly; however, they induced a five to six times higher immune response than oral immunization. The 8-week period between each of the three oral immunizations appeared to be favorable to the anti-HBs humoral responses compared with the shorter schedules. The results presented here clearly identify the importance of low doses of antigen administered orally in extended intervals for a significantly higher anti-HBs response. This finding provides some indications concerning the strategy of orally administered vaccines, including plant-based ones.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Nanogram Doses of Alum-Adjuvanted HBs Antigen Induce Humoral Immune Response in Mice When Orally Administered

Kapusta

Pniewski

Wojciechowicz³

et al. 2010

Arch. Immunol. Ther. Exp.

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“…Plant-produced recombinant HBsAg accumulates intracelluarly as tubular structures in plant cells (Smith et al 2003) and oral immunization with plant material expressing the HBsAg has been shown to be effective in eliciting a strong antibody response to HBV in mice (Kapusta et al 1999;Richter et al 2000;Kong et al 2001) and humans (Kapusta et al 2001). …”

Section: Introductionmentioning

confidence: 98%

Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses

et al. 2006

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A synthetic chimeric gene, TBI-HBS, encoding the immunogenic ENV and GAG epitopes of human immunodeficiency virus (HIV-1) and the surface protein antigen (HBsAg) of hepatitis B virus (HBV), was expressed in tomato plants. Tomato fruits containing the TBI-HBS antigen were fed to experimental mice and, on days 14 and 28 post-feeding, high levels of HIV- and HBV-specific antibodies were present in the serum and feces of the test animals. Intraperitoneal injection of a DNA vaccine directing synthesis of the same TBI-HBsAg antigen boosted the antibody response to HIV in the blood serum; however, it had no effect on the high level of antibodies produced to HBV.

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“…Edible plants containing vaccines can be fed directly to individuals and do not require purification. Transformations have already been reported in a variety of crops, such as tobacco, tomato, and potato [29][30][31], but bananas possess several advantages. Bananas provide one fourth of all food calories in western and central Africa and feed tens of millions in Central America and Asia [32].…”

Section: Candidate Foods As Vehicles For Edible Vaccinesmentioning

confidence: 99%

Novel foods to treat food allergy and gastrointestinal infection

Perr

2004

Curr Gastroenterol Rep

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The gastrointestinal tract communicates directly with the external environment. Necessary nutrients must be absorbed and commensal bacteria tolerated, and foreign proteins, antigens, and pathogens must be simultaneously excluded or destroyed. Immaturity or disruption of the mucosal immune defenses increases vulnerability to food allergy, intolerance, and infectious disease. Diseases resulting from ingested foreign proteins and organisms are increasing and cause morbidity and mortality worldwide. There is no specific treatment for food allergy other than avoidance. Vaccination for infectious disease is limited by the cost and logistics of distribution and administration, particularly in developing countries. Novel strategies are being explored to modulate the gut mucosal immune system by altering protein expression in food. Crops are being developed to remove deleterious allergens to prevent immunogenic exposure while preserving nutritional quality. Local food plants that express protein fragments of pathogens might provide an effective means to stimulate gut mucosal immunity while increasing vaccine accessibility.

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Oral Immunization of Human with transgenic lettuce Expressing Hepatitis B Surface Antigen

Cited by 60 publications

References 4 publications

Nanogram Doses of Alum-Adjuvanted HBs Antigen Induce Humoral Immune Response in Mice When Orally Administered

Nanogram Doses of Alum-Adjuvanted HBs Antigen Induce Humoral Immune Response in Mice When Orally Administered

Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses

Novel foods to treat food allergy and gastrointestinal infection

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