Oral<i>Lactobacillus reuteri</i>LR06 or<i>Bifidobacterium</i>BL5b supplement do not produce analgesic effects on neuropathic and inflammatory pain in rats

Huang, Jiangju; Zhang, Chuanlei; Wang, Jian; Guo, Qulian; Zou, Wangyuan

doi:10.1002/brb3.1260

Cited by 17 publications

(19 citation statements)

References 55 publications

(53 reference statements)

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“…Huang et al [42] attempted to treat neuropathic pain in rats with oral supplementation of probiotics (i.e., Lactobacillus reuteri LR06 or Bifidobacterium BL5b), but failed. Along with the reasons provided by Huang et al [42], we speculate that the treatment failed because key microbes in the rats with neuropathic pain were influenced by and cooperated with the probiotics for survival, and these attenuated the analgesic effects of the probiotics. Interestingly, Staphylococcus, the key microbe in the SNI group, influenced Lactobacillus through cooperative interactions in our study.…”

Section: Discussionmentioning

confidence: 99%

“…Bulgaricus, L. acidophilus, Streptococcus thermophilus) alleviated chemotherapy-induced neuropathic pain in an in vitro model, but has not been tested yet in vivo [12]. A recent study attempted to use probiotics (i.e., Lactobacillus reuteri lr06 or Bifidobacterium bl5b) to alleviate SNI (spared-nerve injury; a traumatic peripheral nerve injury)-induced neuropathic pain, but failed [42].…”

Section: Introductionmentioning

confidence: 99%

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A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

et al. 2020

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Background Neuropathic pain is an abnormally increased sensitivity to pain, especially from mechanical or thermal stimuli. To date, the current pharmacological treatments for neuropathic pain are still unsatisfactory. The gut microbiota reportedly plays important roles in inducing neuropathic pain, so probiotics have also been used to treat it. However, the underlying questions around the interactions in and stability of the gut microbiota in a spared nerve injury-induced neuropathic pain model and the key microbes (i.e., the microbes that play critical roles) involved have not been answered. We collected 66 fecal samples over 2 weeks (three mice and 11 time points in spared nerve injury-induced neuropathic pain and Sham groups). The 16S rRNA gene was polymerase chain reaction amplified, sequenced on a MiSeq platform, and analyzed using a MOTHUR- UPARSE pipeline. Results Here we show that spared nerve injury-induced neuropathic pain alters gut microbial diversity in mice. We successfully constructed reliable microbial interaction networks using the Metagenomic Microbial Interaction Simulator (MetaMIS) and analyzed these networks based on 177,147 simulations. Interestingly, at a higher resolution, our results showed that spared nerve injury-induced neuropathic pain altered both the stability of the microbial community and the key microbes in a gut micro-ecosystem. Oscillospira, which was classified as a low-abundance and core microbe, was identified as the key microbe in the Sham group, whereas Staphylococcus, classified as a rare and non-core microbe, was identified as the key microbe in the spared nerve injury-induced neuropathic pain group. Conclusions In summary, our results provide novel experimental evidence that spared nerve injury-induced neuropathic pain reshapes gut microbial diversity, and alters the stability and key microbes in the gut.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

et al. 2020

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show abstract

“…Numerous studies showed that the supplementation of probiotics is effective for combating various pathogens (Ayala et al, 2019;Dean et al, 2019;Piewngam et al, 2018) and reducing anxiety and depression-related behavior in mice (Bravo et al, 2011). Huang et al (2019) attempted to treat neuropathic pain in rats with oral supplementation of probiotics (i.e., Lactobacillus reuteri LR06 or Bi dobacterium BL5b), but failed. Along with the reasons provided by Huang et al (2019), we speculate that the treatment failed because key microbes in the rats with neuropathic pain were in uenced by and cooperated with the probiotics for survival, and these attenuated the analgesic effects of the probiotics.…”

Section: Discussionmentioning

confidence: 99%

“…Huang et al (2019) attempted to treat neuropathic pain in rats with oral supplementation of probiotics (i.e., Lactobacillus reuteri LR06 or Bi dobacterium BL5b), but failed. Along with the reasons provided by Huang et al (2019), we speculate that the treatment failed because key microbes in the rats with neuropathic pain were in uenced by and cooperated with the probiotics for survival, and these attenuated the analgesic effects of the probiotics. Interestingly, Staphylococcus, the key microbe in the SNI group, in uenced Lactobacillus through cooperative interactions in our study.…”

Section: Discussionmentioning

confidence: 99%

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

Brandon-Mong

Shaw

Chen

et al. 2020

Preprint

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Background Neuropathic pain is an abnormally increased sensitivity to pain, especially from mechanical or thermal stimuli. To date, the current pharmacological treatments for neuropathic pain are still unsatisfactory. The gut microbiota reportedly plays important roles in inducing neuropathic pain, so probiotics have also been used to treat it. However, the underlying questions around the interactions in and stability of the gut microbiota in a spared nerve injury-induced neuropathic pain model and the key microbes (i.e., the microbes that play critical roles) involved have not been answered. Results Here we show that spared nerve injury-induced neuropathic pain alters gut microbial diversity in mice. We successfully constructed reliable microbial interaction networks using the Metagenomic Microbial Interaction Simulator (MetaMIS) and analyzed these networks based on 177,147 simulations. Interestingly, at a higher resolution, our results showed that spared nerve injury-induced neuropathic pain altered both the stability of the microbial community and the key microbes in a gut micro-ecosystem. Oscillospira, which was classified as a low-abundance and core microbe, was identified as the key microbe in the Sham group, whereas Staphylococcus, classified as a rare and non-core microbe, was identified as the key microbe in the spared nerve injury-induced neuropathic pain group. Conclusions In summary, our results provide novel experimental evidence that spared nerve injury-induced neuropathic pain reshapes gut microbial diversity, and alters the stability and key microbes in the gut.

show abstract

“…Bulgaricus, L. acidophilus, Streptococcus thermophilus) alleviated chemotherapy-induced neuropathic pain in an in vitro model, but has not been tested yet in vivo (Castelli et al, 2018). A recent study attempted to use probiotics (i.e., Lactobacillus reuteri lr06 or Bi dobacterium bl5b) to alleviate SNI (spared-nerve injury; a traumatic peripheral nerve injury)-induced neuropathic pain, but failed (Huang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

Brandon-Mong

Shaw

Chen

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Neuropathic pain is an abnormally increased sensitivity to pain, especially from mechanical or thermal stimuli. To date, the current pharmacological treatments for neuropathic pain are still unsatisfactory. The gut microbiota reportedly plays important roles in inducing neuropathic pain, so probiotics have also been used to treat it. However, the underlying questions around the interactions in and stability of the gut microbiota in a spared nerve injury-induced neuropathic pain model and the key microbes (i.e., the microbes that play critical roles) involved have not been answered. We collected 66 fecal samples over two weeks (three mice and 11 time points in spared nerve injury-induced neuropathic pain and Sham groups). The 16S rRNA gene was polymerase chain reaction amplified, sequenced on a MiSeq platform, and analyzed using a MOTHUR- UPARSE pipeline.Results: Here we show that spared nerve injury-induced neuropathic pain alters gut microbial diversity in mice. We successfully constructed reliable microbial interaction networks using the Metagenomic Microbial Interaction Simulator (MetaMIS) and analyzed these networks based on 177,147 simulations. Interestingly, at a higher resolution, our results showed that spared nerve injury-induced neuropathic pain altered both the stability of the microbial community and the key microbes in a gut micro-ecosystem. Oscillospira, which was classified as a low-abundance and core microbe, was identified as the key microbe in the Sham group, whereas Staphylococcus, classified as a rare and non-core microbe, was identified as the key microbe in the spared nerve injury-induced neuropathic pain group. Conclusions: In summary, our results provide novel experimental evidence that spared nerve injury-induced neuropathic pain reshapes gut microbial diversity, and alters the stability and key microbes in the gut.

show abstract

OralLactobacillus reuteriLR06 orBifidobacteriumBL5b supplement do not produce analgesic effects on neuropathic and inflammatory pain in rats

Cited by 17 publications

References 55 publications

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

A network approach to investigating the key microbes and stability of gut microbial communities in a mouse neuropathic pain model

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