Oral hydroxysafflor yellow A reduces obesity in mice by modulating the gut microbiota and serum metabolism

Liu, Juan; Yue, Shi‐Jun; Yang, Zhao; Feng, Wuwen; Meng, Xintong; Wang, Aiting; Peng, Cheng; Wang, Chang‐Yun; Yan, Dan

doi:10.1016/j.phrs.2018.05.012

Cited by 143 publications

(115 citation statements)

References 66 publications

Supporting

Mentioning

109

Contrasting

Unclassified

Order By: Relevance

“…However, Alloprevotella is a newly identified genus (63) and is a short-chain fatty acid (SCFA)-producing bacteria. SCFAs have been shown to play key functional roles in decreasing inflammation and enhancing mucosal barrier function (64). Therefore, the observed alterations in the composition of the intestinal microbiota are in line with the severe colitis observed in MZB1-deficient mice.…”

Section: Discussionmentioning

confidence: 56%

MZB1 promotes the secretion of J-chain–containing dimeric IgA and is critical for the suppression of gut inflammation

Xiong

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

IgA is the most abundantly produced antibody in the body and plays a crucial role in gut homeostasis and mucosal immunity. IgA forms a dimer that covalently associates with the joining (J) chain, which is essential for IgA transport into the mucosa. Here, we demonstrate that the marginal zone B and B-1 cell-specific protein (MZB1) interacts with IgA through the α-heavy-chain tailpiece dependent on the penultimate cysteine residue and prevents the intracellular degradation of α-light-chain complexes. Moreover, MZB1 promotes J-chain binding to IgA and the secretion of dimeric IgA. MZB1-deficient mice are impaired in secreting large amounts of IgA into the gut in response to acute inflammation and develop severe colitis. Oral administration of a monoclonal IgA significantly ameliorated the colitis, accompanied by normalization of the gut microbiota composition. The present study identifies a molecular chaperone that promotes J-chain binding to IgA and reveals an important mechanism that controls the quantity, quality, and function of IgA.

show abstract

Section: Discussionmentioning

confidence: 56%

MZB1 promotes the secretion of J-chain–containing dimeric IgA and is critical for the suppression of gut inflammation

Xiong

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…It was reported by Bao et al that SY could reduce body weight and blood lipid levels in the mice fed with HFD (Bao et al, 2015). Liu et al also found that HSYA could reduce body weight, fat accumulation, and insulin resistance in the HFD-fed mice (Liu et al, 2018). Besides, the promoting effects of SY and HSYA on the expression of Nrf2 and antioxidant enzymes have also been reported in pheochromocytoma cells, HepG2 cells, and…”

Section: Discussionmentioning

confidence: 89%

“…It was reported by Bao et al that SY could reduce body weight and blood lipid levels in the mice fed with high fat diet (Bao et al, 2015). Liu et al also found that HSYA could reduce body weight, fat accumulation, and insulin resistance in the high fat diet-fed mice by modulating the gut microbiota (Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 94%

Safflower Yellow and Its Main Component HSYA Alleviate Diet-Induced Obesity in Mice: Possible Involvement of the Increased Antioxidant Enzymes in Liver and Adipose Tissue

et al. 2020

View full text Add to dashboard Cite

Purpose: Oxidative stress plays an important role in the pathogenesis of obesity and its associated disorders. Safflower yellow (SY) and hydroxysafflor yellow A (HSYA), the natural compounds isolated from Carthamus tinctorius L., has been found to possess antioxidative and anti-obesity properties. The purpose of the present study is to investigate whether SY and its main component HSYA alleviate obesity by the antioxidant effects. Methods: Diet-induced obese (DIO) mice were treated with 200 mg/kg/d SY or HSYA for 10 weeks. Body weight, fat mass, serum biochemical parameters and superoxide dismutase (SOD) activities were measured. Glucose and insulin tolerance tests were performed. The expression of antioxidant enzymes in liver and adipose tissue were measured. In vitro, H 2 O 2-induced oxidative stress HepG2 cells and 3T3-L1 adipocytes were treated with SY and HSYA to investigate the direct effects of SY and HSYA on the expression of antioxidant enzymes. Results: SY and HSYA significantly decreased the body weight gain of DIO mice, and decreased fat mass to 57.8% and 61.6% of the control mice, respectively (P < 0.05). The parameters of glucose metabolism and liver function were improved after SY and HSYA treatment. The hepatic SOD activities and the mRNA levels of antioxidant enzymes in liver and adipose tissue of SY and HSYA treated mice were increased (P < 0.05). Meanwhile, the administration of SY and HSYA on the H 2 O 2-induced oxidative stress HepG2 cells and adipocytes also increased the expression of the antioxidant factor and antioxidant enzymes to 1.2~3.3 folds of the control cells (P < 0.05). Conclusion: SY and its main component HSYA could significantly decrease the fat mass, and improve glucose metabolism and liver function in diet-induced obese mice. The

show abstract

“…suggested that HSYA might be a potential therapeutic drug for obesity, and the gut microbiota may be a potential territory for targeting of HSYA [48]. Furthermore, the half-lives of HSYA are very short and 90% of the HSYA are eliminated within six hours in normal rats; however, HSYA was with high uptake and eliminated slowly in the rats with blood stasis syndrome [49].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Hydroxysafflor Yellow A on Nephropathy by Attenuating Oxidative Stress and Inhibiting Apoptosis in Induced Type 2 Diabetes in Rat

Lee

Zhao

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Diabetic nephropathy (DN) is a serious complication of diabetes mellitus, and its prevalence has been increasing all over the world, which is also the leading cause of end-stage renal failure. Hydroxysafflor yellow A (HSYA) is the main active chemical component of Carthamus tinctorius L., and it is commonly used in patients with cardiovascular and cerebrovascular diseases in China. The aim of this study was to investigate the renal protective effects and molecular mechanisms of HSYA on high-fat diet (HFD) and streptozotocin- (STZ-) induced DN in rats. The DN rats were treated with HSYA for eight weeks. We assessed creatinine (CR), urea nitrogen (UN), glomerular volume, podocyte number, renal inflammation, oxidative stress, and cells apoptosis markers after HSYA treatment. The number of apoptotic cells was measured by the TUNEL assay, and apoptosis-related proteins BAX, caspase-3, and BCL-2 in the renal tissue were analyzed by western blot. The treatment with HSYA significantly decreased fasting blood glucose, CR, UN, and blood lipid profile, including triglyceride and total and low-density lipoprotein cholesterol, even though it did not change the rats’ body weights. The western blot results indicated that HSYA reversed the upregulation of BAX and caspase-3 and significantly increased BCL-2 in renal tissue. Moreover, the levels of TNF-α and the inflammatory products, including free fatty acids (FFA) and lactic dehydrogenase (LDH) in the HSYA group, were significantly decreased. For the oxidative stress marker, the superoxide dismutase (SOD) markedly increased in the HSYA treatment group, while the malondialdehyde (MDA) in the serum and kidney tissue evidently decreased. In conclusion, HSYA treatment preserved kidney function in diabetic nephropathy in the HFD- and STZ-induced rats. The potential mechanism of renal protective effect of HSYA might be through inhibiting oxidative stress, reducing inflammatory reaction, and attenuating renal cell apoptosis. Our studies present a promising use for Hydroxysafflor yellow A in the treatment of type 2 diabetes mellitus.

show abstract

Oral hydroxysafflor yellow A reduces obesity in mice by modulating the gut microbiota and serum metabolism

Cited by 143 publications

References 66 publications

MZB1 promotes the secretion of J-chain–containing dimeric IgA and is critical for the suppression of gut inflammation

MZB1 promotes the secretion of J-chain–containing dimeric IgA and is critical for the suppression of gut inflammation

Safflower Yellow and Its Main Component HSYA Alleviate Diet-Induced Obesity in Mice: Possible Involvement of the Increased Antioxidant Enzymes in Liver and Adipose Tissue

Protective Effect of Hydroxysafflor Yellow A on Nephropathy by Attenuating Oxidative Stress and Inhibiting Apoptosis in Induced Type 2 Diabetes in Rat

Contact Info

Product

Resources

About