Background: Poor oral hygiene and untreated oral diseases and conditions can have a significant impact on the quality of life. Dental students, the future leaders in oral-health care, have a significant role to play in public oral health education and its promotion. Dental students in general have been found to have a positive oral health attitude but their own oral-health behavior must improve if they are want to serve as positive models for their patients, families and friends. Material and Methods: A descriptive cross sectional facultybased study was conducted on December 2021. A total of 92 dental students were involved from Napata College, Dentistry Program, Sudan from (third, fourth and fifth academic level). Data were collected using self-administered questionnaire including questions regarding oral hygiene practice and habits. The data was entered and analyzed using SPSS version 23.Results: Majority of the dental students were female (59)64.1%. The commonest academic level was 3 rd year 37(40.2%), students. The oral hygiene practice among dental students showed that regarding frequency of brushing per day 50(54.3%) of dental students brushed their teeth twice per day, and the type of tooth brush49 (53.3 %) of dental students used medium sized tooth brush and42(45.7%) with circular motion and when asked about changing tooth brush52 (56.5%) changed tooth brush once every three month. Regarding how often participants floss their teeth, the commonest answer was never 45(48.9%), followed by occasionally. 43(46.7%) of dental students went for dental checkup only if they had oral problems .61 (66.3%) of dental students had 1-4 decay teeth. 32(34.8%) ate a sugar containing snack or drink (other than tea and coffee) between meals more than twice daily. Regarding if participants used tobacco products, the majority84 (91.3%) said no. Most of the dental students60(65.2%) were strongly agree that tobacco had adverse effect in the oral and dental tissue.