Changes to the oral cavity arising from malignant disease, treatment and other co-morbidity factors can have a profound affect on the person with cancer, causing pain, discomfort, lack of nutrition, longer hospital stays, and in some situations sepsis and death. While it is recognized that caring for the mouth is an important aspect of cancer care, all too often this aspect of care may be overlooked until problems arise. This leads to needless distress and discomfort and in some cases serious clinical consequences. Oral and gastrointestinal mucositis can affect up to 100% of patients undergoing high-dose chemotherapy and hematopoietic stem cell transplantation. The aim of the study was to assess knowledge and practice on oral care among the patients receiving chemotherapy. This is descriptive cross-sectional study. A total of 102 respondents, visiting B.P. Koirala Memorial Cancer Hospital, Bharatpur Chitwan and who met eligible criteria were systematically sampled and interviewed face to face. Descriptive and inferential statistics were used for the analysis using the SPSS version 16.0. The duration of the study was one month i.e. June, 2010. The tool was developed after reviewing the related literatures. The knowledge on oral care was adequate among 23.4% of the respondents and 18.6% of the respondents had adequate practice receiving chemotherapy. Only 28.4% respondents had adequate knowledge. Among total respondents 60.7% of the respondents had adequate knowledge on the prevention of oral problems and 55.8% of the respondents had adequate knowledge on treatment of oral problems. Nurses were found as a main source of information on oral care. Thus, it was concluded that majority of the respondents had inadequate knowledge and practice on oral care. There was association between selected demographic (age and education). There was no association between family history of cancer and knowledge of respondents. Thus the study also concluded that adequate knowledge on oral care should be provided to the patients before undergoing chemotherapy.