Oral hairy leukoplakia diagnosis by Epstein–Barr virus <i>in situ</i> hybridization in liquid‐based cytology

Braz‐Silva, Paulo Henrique; Santos, R. T. M.; Schussel, Juliana Lucena; Gallottini, Marina

doi:10.1111/cyt.12053

Cited by 13 publications

(10 citation statements)

References 36 publications

(70 reference statements)

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Section: Discussionmentioning

confidence: 99%

“…However, a custom-designed oral brush was warranted (8). We previously collected samples using the "Cytobrush Plus GT" (Medscand Medical AB, Stockholm, Sweden) and "CervexBrush" (Rovers) (8). The observed cellularity of the LBC preparations produced by these brushes ranged from 15,000-40,000 cells with a mean of 25,000 in each slide (8).…”

Section: Discussionmentioning

confidence: 99%

“…We previously collected samples using the "Cytobrush Plus GT" (Medscand Medical AB, Stockholm, Sweden) and "CervexBrush" (Rovers) (8). The observed cellularity of the LBC preparations produced by these brushes ranged from 15,000-40,000 cells with a mean of 25,000 in each slide (8). In the current study, the Brush collected representative cells from all oral mucosa layers; basal/parabasal, spinosal (intermediate) and superficial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported the feasibility and reliability of oral brush sampling for collecting an adequate number of cells from the oral mucosa for subsequent liquid-based cytology (LBC) (5)(6)(7)(8). However, a good, custom-designed brush for oral cavity sampling has been lacking.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of an innovative oral brush for potential applications using liquid based cytology

et al. 2018

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The present study was conducted to assess the applicability of liquid-based cytology (LBC) using an innovative oral brush, Orcellex. Fifty healthy volunteers were recruited. From each subject, four samples were collected using "Orcellex" from apparently normal oral mucosal sites. A plastic spatula was also used to obtain an additional sample. Data on the tolerability and acceptability of the Orcellex were collected from the subjects, together with assessments of the adequacy of LBC slide preparations for cellularity, preparation quality, and the types of cells observed. The Orcellex brush was well accepted by the volunteers, who reported relatively little pain. Orcellex brush LBC preparations were of good quality in terms of cell morphology and staining, with a clean background. Only two smears (2/200; 1%) were found to be inadequate due to low cellularity. All of the plastic spatula LBC preparations were inadequate. Representative cells from all layers of the different oral epithelia examined were documented. Oral liquid-based cytology using the Orcellex brush may have considerable potential for early detection of oral cancer and precancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of an innovative oral brush for potential applications using liquid based cytology

et al. 2018

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“…The diagnosis can be established on a clinical basis, though some authors believe that the presence of EBV should be confirmed by in situ hybridization 8,9 . The importance of an accurate diagnosis of OHL relies in the fact that this condition has a clear prognostic value for HIV infection progression and is classified as a Centers for Disease Control and Prevention category-B clinical marker of HIV disease 10 .…”

Section: Introductionmentioning

confidence: 99%

Retrospective analysis of the clinical behavior of oral hairy leukoplakia in 215 HIV-seropositive patients

et al. 2016

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Oral manifestations are common findings in human immunodeficiency virus (HIV) infected patients and frequently influence the overall health. Oral hairy leukoplakia (OHL) is strongly associated with HIV infection demonstrating its relationship with the individual's immune status and progression of immunosuppression. This study aims to retrospectively evaluate OHL in HIV patients, analyzing its incidence, demographic aspects and possible changes in clinical and epidemiological profile of the disease over 17 years. The records of 1600 HIV-infected patients were reviewed. The data were correlated and analyzed, considering HIV exposure category, age, gender, harmful habits, CD4 level, use and type of antiretroviral. OHL was observed in 215 (13.4%) patients. Most were men in the fourth decade of life, 171 (79.5%) and 112 (52,1%) respectively, but an increase in the incidence of OHL among female patients and those in the fifth decade of life was observed. Tobacco smoking was the most frequent harmful habit reported by 114 (68%) patients. OHL occurred mostly in patients with CD4 counts between 200 and 500 cells/mm3 35 (55.5%). The lower incidence of OHL was found among patients using at least one non-nucleoside reverse transcriptase inhibitor (NNRTI). OHL is related to CD4 count, use of ARVT and tobacco smoking and is also more prevalent in men in the fourth decade of life. These characteristics were recognized in absolute values, but when verifying the behavior over the years we noticed that the incidence of OHL is decreasing and its epidemiological characteristics changing.

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Oral Signs of Viral Disease

Brown

Kollipara

Tyring

2019

Oral Signs of Systemic Disease

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Oral hairy leukoplakia diagnosis by Epstein–Barr virus in situ hybridization in liquid‐based cytology

Abstract: Exfoliative LBC associated with EBV in situ hybridization is a simple, effective and non-invasive diagnostic tool for OHL.

Cited by 13 publications

References 36 publications

Evaluation of an innovative oral brush for potential applications using liquid based cytology

Evaluation of an innovative oral brush for potential applications using liquid based cytology

Retrospective analysis of the clinical behavior of oral hairy leukoplakia in 215 HIV-seropositive patients

Oral Signs of Viral Disease

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