Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers

Masotti, Andrea; Baldassarre, Antonella; Fabrizi, Marta; Olivero, G; Loreti, M. C.; Giammaria, Paola; Veronelli, Patrizio; Graziani, Maria Pia; Manco, Melania

doi:10.1111/ijpo.12133

Cited by 36 publications

(61 citation statements)

References 29 publications

(44 reference statements)

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“…Table 2 also shows the 31/58 miRNAs (25 upexpressed and 6 downexpressed) previously reported to have a similar obese-associated trend in other cohorts [6, 8–14]. Six of these 31 miRNAs showed post-LAGB changes: miR-519d, miR-299-5p, miR-212, and miR-671-3p were downexpressed at T1 versus T0, and miR-370 and miR-487a were upexpressed at T1 versus T0 (Figure 1(a)).…”

Section: Resultssupporting

confidence: 61%

“…Italic type indicates miRNAs whose expression trend was similar to those of previous studies [6, 8–14]. These miRNAs were investigated after LAGB.…”

Section: Figurementioning

confidence: 77%

“…miRNAs whose mean baseline RQ levels were <0.5 (downexpressed) or >2.0 (upexpressed) in all obese patients versus controls were considered differently expressed. We compared the expression of these differently expressed miRNAs with those previously reported in the SAT of other obese cohorts [6, 8–14]. Only miRNAs whose expression trend was confirmed were selected for evaluation after LAGB.…”

Section: Methodsmentioning

confidence: 99%

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Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

et al. 2017

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Background. Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients. To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women. Case Reports. SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0. Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism. Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism. Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0. Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation. Conclusion. We show that the miRNA profile changes after LAGB. This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.

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Section: Resultssupporting

confidence: 61%

“…Italic type indicates miRNAs whose expression trend was similar to those of previous studies [6, 8–14]. These miRNAs were investigated after LAGB.…”

Section: Figurementioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

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Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

et al. 2017

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“…MicroRNAs are small non‐coding RNAs (~22 nucleotides) that function in the post‐transcriptional regulation of gene expression. Several recent studies have evaluated their potential as biomarkers in a variety of paediatric conditions, including diabetes, endothelial dysfunction in obesity, leukaemia, asthma, autism and cholestatic liver disease . In addition to the possibility of improved sensitivity and specificity, miRNAs may appear in the serum earlier in disease progression than many commonly assayed protein biomarkers.…”

Section: Introductionmentioning

confidence: 99%

“…evaluated a full miRNA panel in pre‐pubertal obese children and showed that several miRNAs were de‐regulated and associated with body mass index (BMI), percent fat mass and other measures of metabolic dysfunction . Three specific circulating miRNAs (miR‐200c‐3p, miR‐190a and miR‐95) were identified as biomarkers of insulin resistance in obese preschoolers . Another panel of three miRNAs (hsa‐miR‐125a‐5p, hsa‐miR‐342‐3p and hsa‐miR‐365b‐3p) was predictive of endothelial dysfunction in obese children 5–10 years old .…”

Section: Introductionmentioning

confidence: 99%

Elevation of circulating microRNA levels in obese children compared to healthy controls

et al. 2017

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As childhood obesity increases, it is becoming important to understand the complications of obesity in children and develop novel biomarkers. Evidence indicates that microRNAs (miRNA) are dys-regulated in obesity and may serve as sensitive and specific circulating biomarkers. Non-alcoholic fatty liver disease (NAFLD) is a complication of obesity that ultimately requires a liver biopsy to determine disease severity. While studies have been conducted in adults, no study to date has examined circulating miRNAs in children with obesity and NAFLD. The goal of this study was to evaluate a panel of selected circulating miRNAs in obese children compared to healthy controls. We present here an analysis of a pre-selected panel of 20 candidate miRNAs in obese children compared to healthy controls. The miRNAs were chosen based on having been previously reported to be involved in NAFLD. We found that 16 out of 20 miRNAs tested were elevated at least twofold in children with obesity compared to controls. miR-122 and miR-199a showed the greatest increase in children with obesity versus controls. Both also had a high area under the curve when receiver-operator curves were plotted. Several circulating miRNAs correlated with body mass index (BMI) or serum transaminases. This study provides initial evidence that circulating miRNAs can be measured in the paediatric population and provides several diagnostic candidates increased in children with obesity that may be relevant to NAFLD.

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The downregulation of miR‐22 and miR‐372 may contribute to gestational diabetes mellitus through regulating glucose metabolism via the PI3K/AKT/GLUT4 pathway

Li¹,

Yuan

et al. 2022

Clinical Laboratory Analysis

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Background: Identifying effective regulatory mechanisms will be significant for Gestational diabetes mellitus (GDM) diagnosis and treatment. Methods:The expressions of miR-22 and miR-372 in placenta tissues from 75 pregnant women with GDM and 75 matched healthy controls and HRT8/SVneo cells (a model of insulin resistance) were analyzed by qPCR. The expressions of PI3K, AKT, IRS, and GLUT4 in high glucose-treated HRT8/SVneo cells transfected with miR-22 or miR-372 mimics or inhibitors was assessed by Western blot. A luciferase gene reporter assay was employed to verify miRNAs' target genes. Results:The expressions of miR-22 and miR-372 in placental tissues from GDM patients and HRT8/SVneo cells were significantly decreased compared with the respective controls. The GLUT4 expression was significantly decreased in the placenta tissues of GDM and HRT8/SVneo cells with high glucose transfected with miR-22 and miR-372 inhibitors. We confirmed that SLC2A4, the gene encoding GLUT4, was a direct target of miR-22 and miR-372. In this study, we report that the lower expressions of miR-22 and miR-372 in placental tissue from GDM patients. Conclusion:Our results further suggested that the downregulations of miR-22 and miR-372 may contribute to GDM through regulating the PI3K/GLUT4 pathway.

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Oral glucose tolerance test unravels circulating miRNAs associated with insulin resistance in obese preschoolers

Cited by 36 publications

References 29 publications

Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding

Elevation of circulating microRNA levels in obese children compared to healthy controls

The downregulation of miR‐22 and miR‐372 may contribute to gestational diabetes mellitus through regulating glucose metabolism via the PI3K/AKT/GLUT4 pathway

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