Oral formulation of Wnt inhibitor complex reduces inflammation and fibrosis in intraperitoneal implants in vivo

Santos, Ana Luíza de Castro; Silva, Natália Jordana Alves da; Viana, Celso Tarso Rodrigues; Santos, Letícia Cristine Cardoso Dos; Silva, Gabriel Henrique Costa da; Scalzo, Sérgio; Costa, P.A.C.R.; Silva, Walison Nunes da; Jesus, Itamar Couto Guedes de; Birbrair, Alexander; Magalhães, Mariana T.Q. de; Frézard, Frédéric; Guatimosim, Sílvia; Haley, Rebecca M; Mitchell, Michael J.; Andrade, Sílvia Passos; Campos, Paula Peixoto; Guimarães, Pedro Pires Goulart

doi:10.1007/s13346-023-01303-0

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“…Sections with a thickness of 5 μm were stained with H&E for light microscopic studies to characterize the fibrovascular tissue and quantify morphometrically blood vessels, foreign body giant cells, and capsule thickness. In order to assess the level of inflammation, 20 fields per slide from 5 animals per group were evaluated according to the following score parameters: committed area, polymorphonuclear and mononuclear cell infiltration, and extent of each parameter (1 < 25%, 2 ≥ 25% to <50%, 3 ≥ 50% to <75%, 4 ≥ 75%), in a 1° to 4° (Supplementary Material 2) as previously described by Santos et al 44 and Feng et al 45 (Supplementary Material 2A,B).…”

Section: Methodsmentioning

confidence: 99%

Amitriptyline efficacy in decreasing implant‐induced foreign body reaction

et al. 2023

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Beyond its actions on the nervous system, amitriptyline (AM) has been shown to lower inflammatory, angiogenic, and fibrogenic markers in a few pathological conditions in human and in experimental animal models. However, its effects on foreign body reaction (FBR), a complex adverse healing process, after biomedical material implantation are not known. We have evaluated the effects of AM on the angiogenic and fibrogenic components on a model of implant‐induced FBR. Sponge disks were implanted subcutaneously in C57BL/6 mice, that were treated daily with oral administration of AM (5 mg/kg) for seven consecutive days in two protocols: treatment was started on the day of surgery and the implants were removed on the seventh day after implantation and treatment started 7 days after implantation and the implants removed 14 after implantation. None of the angiogenic (vessels, Vascular endothelial growth factor (VEGF), and interleukin‐1β (IL‐1β) or fibrogenic parameters (collagen, TGF‐β, and fibrous capsule) and giant cell numbers analyzed were attenuated by AM in 7‐day‐old implants. However, AM was able to downregulate angiogenesis and FBR in 14‐day‐old implants. The effects of AM described here expands its range of actions as a potential agent capable of attenuating fibroproliferative processes that may impair functionality of implantable devices.

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Section: Methodsmentioning

confidence: 99%