Oral feeding and nasal instillation immunization with Microtus brandti lactate dehydrogenase c epitope DNA vaccine reduces fertility in mice via specific antibody responses

Shi, Shu-qun; Wang, Jinling; Peng, Jing‐Pian; Chang, Jian-Jun; Yang, Ying

doi:10.1016/j.fertnstert.2005.03.060

Cited by 7 publications

(6 citation statements)

References 9 publications

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“…For the reproduction activities, the local humoral immune response might have a more direct effect in lowing the rate of pregnancy. Shi et al immunized mice by oral feeding and nasal instillation with LDHC4 DNA vaccine and the effect was also significant [9] . Mucosal immunization could induce both local mucosal and systemic immune responses.…”

Section: Discussionmentioning

confidence: 99%

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Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immunocontraceptive effect on mice

et al. 2008

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Lactate dehydrogenase C4 (LDHC4) is a key enzyme for sperm metabolism. It is distributed specifically in testis and is highly immunogenic. In this study, two DNA vaccines pVAX1-hLDHC and pVAX1-mLDHC were constructed by inserting coding sequences of human and mice LDHC4 into the eukaryotic expression vector pVAX1. The production of LDHC4 specific antibodies was induced in the sera of vaccinated mice and the reproductive tract secretions of vaccinated female mice through immunization by mucosal surface instillation. Furthermore, the antibody titer increased with the times of immunization. In the mating experiment, the number of newborns of the vaccinated mice reduced significantly and some immunized female mice even lost the ability to bear any offsprings, suggesting that the difference between the immunized and control mice was statistically significant. Sperm agglutination analysis indicated that both the antisera from immunized mice and the reproductive tract secretions of vaccinated female mice could agglutinate normal sperms. Results of immunohistochemistry showed that the antibodies present in the sera of immunized mice and the reproductive tract secretions of vaccinated female mice could specifically react with LDHC4 antigen, which mainly locates in the cytoplasm, acrosome membrane externa and acrosome capsule of the sperm. Taken together, our results indicated that the constructed contraceptive DNA vaccines did yield immunocontraceptive effects on mice and this would enable clinical trials in near future.

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Section: Discussionmentioning

confidence: 99%

“…After vaginal DNA immunization using a controlled delivery matrix, this DNA vaccine could induce the production of specific antibodies in mice. Shi et al [9] and Chang et al [10] also constructed the LDHC4 DNA vaccine and studied its effect of immunocontraception.…”

mentioning

confidence: 99%

Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immunocontraceptive effect on mice

et al. 2008

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“…Anti‐sperm antibodies has been found in infertility patients 54 . Considerable effort has been made to identify sperm proteins as possible targets for oral immunocontraceptive vaccines and these include: tsNHE, 55 LDH, 44,56 SP10, 23,24 hSMP‐1, 57 FSA‐1 13 . The availability of ZP and sperm antigens is mainly through recombinant DNA technology.…”

Section: Introductionmentioning

confidence: 99%

Oral immunocontraceptive vaccines: A novel approach for fertility control in wildlife

Yang

Zhou

Li³

et al. 2022

American J Rep Immunol

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The overabundant populations of wildlife have caused many negative impacts, such as human‐wildlife conflicts and ecological degradation. The existing approaches like injectable immunocontraceptive vaccines and lethal methods have limitations in many aspects, which has prompted the advancement of oral immunocontraceptive vaccine. There is growing interest in oral immunocontraceptive vaccines for reasons including high immunization coverage, easier administration, frequent boosting, the ability to induce systemic and mucosal immune responses, and cost‐effectiveness. Delivery systems have been developed to protect oral antigens and enhance the immunogenicity, including live vectors, microparticles and nanoparticles, bacterial ghosts, and mucosal adjuvants. However, currently, no effective oral immunocontraceptive vaccine is available for field trials because of the enormous development challenges, including biological and physicochemical barriers of the gastrointestinal tract, mucosal tolerance, pre‐existing immunity, antigen residence time in the small intestine, species specificity and other safety issues. To overcome these challenges, this article summarizes achievements in delivery systems and contraceptive antigens in oral immunocontraceptive vaccines and explores the potential barriers for future vaccine design and application.

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“…Only one form of the isoenzyme is expressed in these tissues: LDH-C4. 5) Antibodies against LDH-C4 6-7) and DNA vaccines based on LDH-C gene 8) effectively reduced the reproductive capacity of many mammals. Gene knockout experiments showed that the lack of LDH-C could seriously damage the reproductive capacity of male mice.…”

mentioning

confidence: 99%

Inhibitor screening of lactate dehydrogenase C4 from black-lipped pika in the Western Sichuan Plateau

Zhang

Xue

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Oral feeding and nasal instillation immunization with Microtus brandti lactate dehydrogenase c epitope DNA vaccine reduces fertility in mice via specific antibody responses

Cited by 7 publications

References 9 publications

Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immunocontraceptive effect on mice

Construction of sperm-specific lactate dehydrogenase DNA vaccine and experimental study of its immunocontraceptive effect on mice

Oral immunocontraceptive vaccines: A novel approach for fertility control in wildlife

Inhibitor screening of lactate dehydrogenase C4 from black-lipped pika in the Western Sichuan Plateau

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