Oral exposure to the anti-pyridoxine compound 1-amino d-proline further perturbs homocysteine metabolism through the transsulfuration pathway in moderately vitamin B6 deficient rats

Investigation of vitamin B6 inadequacy, induced by exposure to the anti-B6 factor 1-amino d-proline, on plasma lipophilic metabolites of rats: a metabolomics approach

“…Therefore, potential effects of B 6 inadequacy on other metabolic pathways including neurotransmitter biosynthesis, organic acids and lipid metabolism need to be further investigated. Additionally, we also had demonstrated that a concurrent feeding of antivitamin B 6 during low vitamin B 6 status further perturbed homocysteine metabolism and initiated hepatic steatosis due to increased accumulation of liver triglycerides [24]. In view of these, the current study was designed to develop novel lipophilic biomarkers of B 6 inadequacy induced by oral exposure to anti-B 6 factor 1ADP, in order to understand better about the roles of this water-soluble vitamin on other biochemical pathways particularly linked to lipid metabolism.…”

“…Food intake was monitored daily, and body weight was measured weekly over a 35-day study period. Full details on the model and the confirmation of moderate B 6 deficiency have been presented elsewhere [24]. In brief, feeding of 0.7 mg/kg diet of PN·HCl decreased plasma PLP to a level of 30-40 nmoles/L without any significant changes in food consumption, growth and plasma homocysteine (<12 µmol/L) compared with the optimum B 6 supply (7 mg/kg diet of PN·HCl).…”

“…This condition was considered to be a moderate vitamin B 6 deficiency in a rodent model, and hence, a dietary supply of 0.7 mg/kg diet of PN·HCl was chosen to mimic a moderate vitamin B 6 deficiency in humans for this study. Additionally, we chose 10 mg/kg diet of 1ADP in the current study as this antivitamin B 6 at this concentration impaired growth performance as well as profiles of several B 6 markers including plasma PLP content in the rats of a model previously described [24]. At the end of the experiment, rats were fasted for 12 h and euthanized using isoflurane.…”

Impaired recovery of hind limb muscle perfusion following ischemic injury in rodents receiving B vitamin supplementation

“…Plasma B 6, determined as the semicarbazide derivative of pyridoxyl-5-phosphate, was measured using high-performance liquid chromatography (HPLC) and fluorescence detection as previously described [21]. Plasma total Hcy and Cys were quantified using ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate by HPLC with fluorescence detection [21,22].…”

“…Plasma total Hcy and Cys were quantified using ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate by HPLC with fluorescence detection [21,22]. Vitamin B12 and folate were measured using paramagnetic particle, chemiluminescent immunoassays (Access Immunoassay Systems, Beckman Coulter Inc.) according to standardized processes at the St. Michael's Hospital Core Laboratory.…”