Oral Epithelial Cells are Activated <i>via</i> TRP Channels

Wang, B.; Danjo, Atsushi; Kajiya, Hiroshi; Okabe, Koji; Kido, Mizuho A.

doi:10.1177/0022034510385459

Cited by 46 publications

(41 citation statements)

References 25 publications

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“…In the present study, we confirmed TRPV4 protein expression by in situ hybridization, Western blotting, immunohistochemistry, and immunocytochemistry, in accord with our previous reports on TRPV4 mRNA expression in rats and mice . Antibody specificity against TRP channels has challenged many researchers, including us.…”

Section: Discussionsupporting

confidence: 91%

Enhanced junctional epithelial permeability in TRPV4‐deficient mice

Kitsuki

Yoshimoto

Aijima

et al. 2019

J of Periodontal Research

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Background and Objective As the interface between the oral cavity and the teeth, the junctional epithelial barrier is critical for gingival defense. The junctional epithelium is subject to mechanical stresses from biting force or external insults such as bacterial attacks, but little is known about the effects of mechanical stimuli on epithelial functions. Transient receptor potential vanilloid 4 (TRPV4) functions as a mechanosensitive nonselective cation channel. In the present study, based on marked expression of TRPV4 in the mouse junctional epithelium, we aimed to clarify the putative links between TRPV4 and junctional complexes in the junctional epithelium. Methods and Results Histological observations revealed that the junctional epithelium in TRPV4‐deficient (TRPV4−/−) mice had wider intercellular spaces than that in wild‐type (TRPV4+/+) mice. Exogenous tracer penetration in the junctional epithelium was greater in TRPV4−/− mice than in TRPV4+/+ mice, and immunoreactivity for adherens junction proteins was suppressed in TRPV4−/− mice compared with TRPV4+/+ mice. Analysis of a mouse periodontitis model showed greater bone volume loss in TRPV4−/− mice compared with TRPV4+/+ mice, indicating that an epithelial barrier deficiency in TRPV4−/− mice may be associated with periodontal complications. Conclusion The present findings identify a crucial role for TRPV4 in the formation of adherens junctions in the junctional epithelium, which could regulate its permeability. TRPV4 may be a candidate pharmacological target to combat periodontal diseases.

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Section: Discussionsupporting

confidence: 91%

Enhanced junctional epithelial permeability in TRPV4‐deficient mice

Kitsuki

Yoshimoto

Aijima

et al. 2019

J of Periodontal Research

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“…Earlier publications had suggested a role for the direct activation of oral epithelial cells by polyphenols (Wang et al . ; Kurogi et al . ).…”

Section: Discussionmentioning

confidence: 99%

Do Transient Receptor Protein (TRP) Channels Play a Role in Oral Astringency?

Carpenter

2013

Journal of Texture Studies

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Astringency is the dry sensation when solutions containing polyphenols are taken into the mouth. It is believed that polyphenols interact with salivary proteins to disrupt the lubricating layer, thus causing the perceived dryness. However, some recent data have suggested that the oral epithelial cells may be directly stimulated by polyphenols by activating transient receptor protein (TRP) channels. To test this theory, TRP channels in immortalized oral epithelial cells (TR146) were incubated with TRP agonists and black tea. In addition, human oral epithelial cells were collected and stimulated. Whereas the TR146 cells responded to known TRP agonists (camphor, capsaicin and menthol), they did not respond to the tea solution. In addition, nearly all the human oral epithelial cells did not respond to either the TRP agonists or the tea solution. It is concluded that human oral epithelial cells are not directly stimulated by polyphenols during the normal drinking of black tea. Practical Applications There is an increasing awareness of the role of transient receptor protein (TRP) receptors being important for various taste modalities. Because TRP channels occur in most epithelial cells, it was important to check to see if human oral epithelial cells have functional TRP channels. It appears that the top layer of cells does not, however, some sensory nerves and some deeper epithelial cells may have functional channels. This is important for any tastant that may affect TRP channels, particularly those described as having a trigeminal effect.

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“…Expression patterns of TRPV1, 3, and 4 have previously been reported in normal tongue, buccal mucosa, and palate tissue of rats [19], while TRPV3 is reported as involved in healing mucosal injury in rat palatal mucosa [20]. In humans, quantitative expression analysis of TRPV1 has been reported in the lingual mucosa and oral squamous cell carcinoma (SCC) [16, 21].…”

Section: Introductionmentioning

confidence: 99%

Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking

Sakakibara

Kusumoto

et al. 2017

PLoS ONE

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ObjectivesTransient receptor potential cation channel (subfamily V, members 1–4) (TRPV1–4) are expressed in skin and neurons and activated by external stimuli in normal mucosae of all oral cavity sites. The oral cavity is exposed to various stimuli, including temperature, mechanical stimuli, chemical substances, and changes in pH, and, notably, the risk factors for oncogenic transformation in oral squamous epithelium are the same as the external stimuli received by TRPV1–4 receptors. Hence, we examined the relationship between oral squamous cell carcinoma (SCC) and TRPV1–4 expression.Materials and MethodsOral SCC patients (n = 37) who underwent surgical resection were included in this study. We investigated the expression of TRPV1–4 by immunohistochemical staining and quantification of TRPV1–4 mRNA in human oral mucosa. In addition, we compared the TRPV1–4 levels in mucosa from patients with SCC to those in normal oral mucosa.ResultsThe receptors were expressed in oral mucosa at all sites (tongue, buccal mucosa, gingiva, and oral floor) and the expression was stronger in epithelia from patients with SCC than in normal epithelia. Furthermore, alcohol consumption and tobacco use were strongly associated with the occurrence of oral cancer and were found to have a remarkable influence on TRPV1–4 receptor expression in normal oral mucosa. In particular, patients with a history of alcohol consumption demonstrated significantly higher expression levels.ConclusionVarious external stimuli may influence the behavior of cancer cells. Overexpression of TRPV1-4 is likely to be a factor in enhanced sensitivity to external stimuli. These findings could contribute to the establishment of novel strategies for cancer therapy or prevention.

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Oral Epithelial Cells are Activated via TRP Channels

Cited by 46 publications

References 25 publications

Enhanced junctional epithelial permeability in TRPV4‐deficient mice

Enhanced junctional epithelial permeability in TRPV4‐deficient mice

Do Transient Receptor Protein (TRP) Channels Play a Role in Oral Astringency?

Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking

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