Oral dose and faecal concentration of antibiotics during antibiotic decontamination in mice and in a patient

Waaij, D. van der; Vries, J. M. Berghuis de; Altes, C. Korthals

doi:10.1017/s0022172400024025

Cited by 24 publications

(15 citation statements)

References 9 publications

(6 reference statements)

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“…Mice were allowed to acclimate to the animal facility for 1‐week prior to the start of the experiment. To deplete the commensal microbiota, at 12 weeks of age mice were treated with broad‐spectrum antibiotics: ampicillin 1.0 g/L (Sigma, St. Louis, MO, USA) and neomycin 0.5 g/L (Sigma), which are poorly absorbed by the intestine . Fresh antibiotics were given weekly via sterile drinking water; the effectiveness of the antibiotic‐water was confirmed by its prevention of bacterial growth on agar plates.…”

Section: Methodsmentioning

confidence: 99%

Probiotic Lactobacillus reuteri Prevents Postantibiotic Bone Loss by Reducing Intestinal Dysbiosis and Preventing Barrier Disruption

Schepper

Collins

Rios‐Arce³

et al. 2019

Journal of Bone and Mineral Research

139

154

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Antibiotic treatment, commonly prescribed for bacterial infections, depletes and subsequently causes long‐term alterations in intestinal microbiota composition. Knowing the importance of the microbiome in the regulation of bone density, we investigated the effect of postantibiotic treatment on gut and bone health. Intestinal microbiome repopulation at 4‐weeks postantibiotic treatment resulted in an increase in the Firmicutes:Bacteroidetes ratio, increased intestinal permeability, and notably reduced femoral trabecular bone volume (approximately 30%, p < 0.01). Treatment with a mucus supplement (a high‐molecular‐weight polymer, MDY‐1001 [MDY]) prevented the postantibiotic‐induced barrier break as well as bone loss, indicating a mechanistic link between increased intestinal permeability and bone loss. A link between the microbiome composition and bone density was demonstrated by supplementing the mice with probiotic bacteria. Specifically, Lactobacillus reuteri, but not Lactobacillus rhamnosus GG or nonpathogenic Escherichia coli, reduced the postantibiotic elevation of the Firmicutes:Bacteroidetes ratio and prevented femoral and vertebral trabecular bone loss. Consistent with causing bone loss, postantibiotic‐induced dysbiosis decreased osteoblast and increased osteoclast activities, changes that were prevented by both L. reuteri and MDY. These data underscore the importance of microbial dysbiosis in the regulation of intestinal permeability and bone health, as well as identify L. reuteri and MDY as novel therapies for preventing these adverse effects. © 2018 American Society for Bone and Mineral Research.

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Section: Methodsmentioning

confidence: 99%

Probiotic Lactobacillus reuteri Prevents Postantibiotic Bone Loss by Reducing Intestinal Dysbiosis and Preventing Barrier Disruption

Schepper

Collins

Rios‐Arce³

et al. 2019

Journal of Bone and Mineral Research

139

154

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“…Similar treatment protocols have been followed previously in comparable studies in mice and rats, demonstrating the induction of significant changes of the GCM. 18,19,57 Experimental protocols Mice were treated with the antibiotic mixture (n D 28) or vehicle (n D 28) during 14 consecutive days. Different subgroups of vehicle-and antibiotics-treated animals were used to assess visceral pain responses (behavioral pain responses to IP acetic acid, n D 22 and IC capsaicin, n D 22) and in vitro colonic contractility (n D 12).…”

Section: Antibiotic Treatmentmentioning

confidence: 99%

Antibiotic-induced dysbiosis alters host-bacterial interactions and leads to colonic sensory and motor changes in mice

2015

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Abbreviations: AMP, antimicrobial peptide; CB1/2, cannabinoid receptor type 1 or 2; FGD, functional gastrointestinal disorder; FISH, fluorescent in situ hybridization; GCM, gut commensal microbiota; GI, gastrointestinal; IBS, irritable bowel syndrome; iNOS, inducible nitric oxide synthase; MOR, mu-opioid receptor; NGF, nerve growth factor; PPR, pattern recognition receptor; Reg3g, regenerating islet-derived protein 3 gamma; RELMb, resistin-like molecule-b; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SFB, segmented filamentous bacteria; sIgA, secretory IgA; TLR, toll-like receptor; TPH 1/2, tryptophan hydroxylase isoforms 1 or 2; TRPV1/3, transient receptor potential vanilloid types 1 or 3Alterations in the composition of the commensal microbiota (dysbiosis) seem to be a pathogenic component of functional gastrointestinal disorders, mainly irritable bowel syndrome (IBS), and might participate in the secretomotor and sensory alterations observed in these patients.We determined if a state antibiotics-induced intestinal dysbiosis is able to modify colonic pain-related and motor responses and characterized the neuro-immune mechanisms implicated in mice. A 2-week antibiotics treatment induced a colonic dysbiosis (increments in Bacteroides spp, Clostridium coccoides and Lactobacillus spp and reduction in Bifidobacterium spp). Bacterial adherence was not affected. Dysbiosis was associated with increased levels of secretory-IgA, up-regulation of the antimicrobial lectin RegIIIg, and toll-like receptors (TLR) 4 and 7 and down-regulation of the antimicrobial-peptide Resistin-Like Molecule-b and TLR5. Dysbiotic mice showed less goblet cells, without changes in the thickness of the mucus layer. Neither macroscopical nor microscopical signs of inflammation were observed. In dysbiotic mice, expression of the cannabinoid receptor 2 was up-regulated, while the cannabinoid 1 and the mu-opioid receptors were down-regulated. In antibiotic-treated mice, visceral painrelated responses elicited by intraperitoneal acetic acid or intracolonic capsaicin were significantly attenuated. Colonic contractility was enhanced during dysbiosis. Intestinal dysbiosis induce changes in the innate intestinal immune system and modulate the expression of pain-related sensory systems, an effect associated with a reduction in visceral painrelated responses. Commensal microbiota modulates gut neuro-immune sensory systems, leading to functional changes, at least as it relates to viscerosensitivity. Similar mechanisms might explain the beneficial effects of antibiotics or certain probiotics in the treatment of IBS.

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“…Experiments were performed in accordance with the Netherlands Experiments on Animals Act (1977) In a separate experiment on melphalan and IMS, the recipient mice were maintained on the non-absorbable antibiotic neomycin sulphate (3.5 g 1-; E-Z-EM Rooster, Dordrecht, The Netherlands), administered in drinking water 7 days before and 14 days after treatment. It is estimated that this dose and course of antibiotic treatment completely suppressed the intestinal microflora at the time of treatment (Van der Waaij & Berghuis-de Vries, 1974;Goris et al, 1986a) and is capable of protecting mice from radiationinduced gut lethality (Pearson & Phelps, 1981).…”

Section: Animalsmentioning

confidence: 99%

Relationships between ablation of distinct haematopoietic cell subsets and the development of donor bone marrow engraftment following recipient pretreatment with different alkylating drugs

Down

Boudewijn²,

Dillingh³

et al. 1994

Br J Cancer

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(LTBMCs). At this time a fixed complement of 10 congenicallv marked donor bone marrow cells (B6-Gpi-10-* B6-Gpi-PI) was infused in the drug-treated mice and erythroid engraftment was followed over 36 weeks. Diverse effects on early-and late-developing CAFC frequencies were found for the different drugs: these were generally related to the pattern of donor bone marrow engraftment in treated recipients. Melphalan was more toxic to earlydeveloping than to late-developing CAFC subsets. and the transplant only offered an earlv wave of blood chimerism followed bv return of host cells. CY and BCNU had minimal to moderate effects on CAFC content and engraftment with no apparent preference for any particular haemopoietic cell subset. IMS also had a relatively low toxic effect on host marrow CAFC frequencies but appeared exceptional in its ability to allow for more donor-type engraftment. The dimethane sulphonate compounds busulphan and DMB were especially potent at depleting late CAFC subsets and ensured high and lasting levels of donor bone marrow engraftment. These studies support the value of CAFC measurements for predicting the fate and growth of transplanted bone marrow cells in recipients pretreated with a variety of cytotoxic agents.

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Oral dose and faecal concentration of antibiotics during antibiotic decontamination in mice and in a patient

Cited by 24 publications

References 9 publications

Probiotic Lactobacillus reuteri Prevents Postantibiotic Bone Loss by Reducing Intestinal Dysbiosis and Preventing Barrier Disruption

Probiotic Lactobacillus reuteri Prevents Postantibiotic Bone Loss by Reducing Intestinal Dysbiosis and Preventing Barrier Disruption

Antibiotic-induced dysbiosis alters host-bacterial interactions and leads to colonic sensory and motor changes in mice

Relationships between ablation of distinct haematopoietic cell subsets and the development of donor bone marrow engraftment following recipient pretreatment with different alkylating drugs

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