Oral DNA vaccines based on CS-TPP nanoparticles and alginate microparticles confer high protection against infectious pancreatic necrosis virus (IPNV) infection in trout

Ahmadivand, Sohrab; Soltani, Mehdi; Behdani, Mahdi; Evensen, Øystein; Alirahimi, Ehsan; Hassanzadeh, Reza; Soltani, Ellahe

doi:10.1016/j.dci.2017.05.004

Cited by 58 publications

(32 citation statements)

References 44 publications

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“…Antigen-specific antibodies are an important parameter of the humoral immune response following vaccination and offer the most reliable correlates with protection, in conferring protective immunity for immunized fish (28). Previous studies have suggested that intramuscular or oral administration of DNA vaccines can lead to the production of antigen-specific antibodies against bacteria and recombinant proteins, with the highest level of antiserum being observed between weeks 5 and 7 post-vaccination (62)(63)(64)(65)(66)(67). In the present study, production of specific antibodies against V. anguillarum or rVAA was detected at days 28 and 35 post-immunization.…”

Section: Discussionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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Vibrio anguillarum is a pathogenic bacterium that infects flounder resulting in significant losses in the aquaculture industry. The VAA protein previously identified in flounder is associated with a role in immune protection within these fish. In the present study, a recombinant DNA plasmid encoding the VAA gene of V. anguillarum was constructed and its potential as a DNA vaccine, to prevent the infection of V. anguillarum in flounder fish, investigated. We verified the expression of the VAA protein both in vitro in cell lines and in vivo in flounder fish. The protective effects of pcDNA3.1-VAA (pVAA) were analyzed by determination of the percentage of sIgM+, CD4-1+, CD4-2+, CD8β+ lymphocytes, and the production of VAA-specific antibodies in flounder following their immunization with the DNA vaccine. Histopathological changes in immune related tissues, bacterial load, and relative percentage survival rates of flounder post-challenge with V. anguillarum, were all investigated to assess the efficacy of the pVAA DNA vaccine candidate. Fish intramuscularly immunized with pVAA showed a significant increase in CD4-1+, CD4-2+, and CD8β+ T lymphocytes at days 9, 11, and 14 post-vaccination, reaching peak T-cell levels at days 11 or 14 post-immunization. The percentage of sIgM+ lymphocytes reached peak levels at weeks 4–5 post-immunization. Specific anti-V. anguillarum or anti-rVAA antibodies were induced in inoculated fish at days 28–35 post-immunization. The liver of vaccinated flounder exhibited only slight histopathological changes compared with a significant pathology observed in control immunized fish. Additionally, a lower bacterial burden in the liver, spleen, and kidney were observed in pVAA protected fish in response to bacterial challenge, compared with pcDNA3.1 vector control injected fish. Moreover, the pVAA vaccine confers a relative percentage survival of 50.00% following V. anguillarum infection. In summary, this is the first study indicating an initial induction of the T lymphocyte response, followed by B lymphocyte induction of specific antibodies as a result of DNA immunization of flounder. This signifies the important potential of pVAA as a DNA vaccine candidate for the control of V. anguillarum infection.

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Section: Discussionmentioning

confidence: 99%

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Xing

Tang

et al. 2019

Front. Immunol.

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“…High levels of protection against IPNv in salmonids have been obtained through various approaches, such as subunit or DNA vaccines, oral administration, and using a liposomal formulation of chitosan/tripolyphosphate nanoparticles and alginate microparticles (C arcamo et al 2012;Ahmadivand et al 2017;Reyes et al 2017).…”

Section: Ipnv: Vaccination In Chilementioning

confidence: 99%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

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The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

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“…The infectious hematopoietic necrosis virus (IHNV) is a member of the Novirhabdovirus genus in the Rhabdoviridae family of viruses (Ahmadivand, Soltani, Behdani et al, 2017;Ahmadivand, Soltani, Mardani et al, 2017). Outbreaks of IHNV infection, leading to mass mortality, have posed a significant threat to the salmonid farming industry worldwide (Rucker, Whipple, Parvin, & Evans, 1953).…”

Section: Introductionmentioning

confidence: 99%

“…These epitopes are capable of eliciting the production of viral neutralizing antibodies against IPNV (Frost et al, 1995;Munang'Andu & Evensen, 2015). Vaccines encoding the recombinant VP2 gene had been shown to be sufficient to induce a protective immune response (Ahmadivand, Soltani, Behdani et al, 2017;Ahmadivand, Soltani, Mardani et al, 2017;Ballesteros, Saint-Jean, Encinas, Perez-Prieto, & Coll, 2012;Guo et al, 2018). A previous study showed that the truncated VP2 protein (147-307 amino acids) is capable of inducing an anti-IPNV antibody response with antibody titres similar to those for whole IPNV (Labus et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐G^N438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout

Chen

Guo

Wang

et al. 2019

Journal of Fish Diseases

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Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens in rainbow trout farming worldwide. Their co‐infection is also common, which causes great economic loss in juvenile salmon species. Development of a universal virus vaccine providing broadly cross‐protective immunity will be of great importance. In this study, we generated two recombinant (r) virus (rIHNV‐N438A‐ΔNV‐EGFP and rIHNV‐N438A‐ΔNV‐VP2) replacing the NV gene of the backbone of rIHNV at the single point mutation at residue 438 with an efficient green fluorescent protein (EGFP) reporter gene and antigenic VP2 gene of IPNV. Meanwhile, we tested their efficacy against the wild‐type (wt) IHNV HLJ‐09 virus and IPNV serotype Sp virus challenge. The relative per cent survival rates of two recombinant viruses against (wt) IHNV HLJ‐09 virus challenge were 84.6% and 81.5%, respectively. Simultaneously, the relative per cent survival rate of rIHNV‐N438A‐ΔNV‐VP2 against IPNV serotype Sp virus challenge was 88.9%. It showed the two recombinant viruses had high protection rates and induced a high level of antibodies against IHNV or IPNV. Taken together, these results suggest the VP2 gene of IPNV can act as candidate gene for vaccine and attenuated multivalent live vaccines and molecular marker vaccines have potential application for viral vaccine.

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Oral DNA vaccines based on CS-TPP nanoparticles and alginate microparticles confer high protection against infectious pancreatic necrosis virus (IPNV) infection in trout

Cited by 58 publications

References 44 publications

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐G^N438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout

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Oral DNA vaccines based on CS-TPP nanoparticles and alginate microparticles confer high protection against infectious pancreatic necrosis virus (IPNV) infection in trout

Cited by 58 publications

References 44 publications

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

A DNA Vaccine Encoding the VAA Gene of Vibrio anguillarum Induces a Protective Immune Response in Flounder

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐GN438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout

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Resources

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Immunity induced by recombinant attenuated IHNV (infectious hematopoietic necrosis virus)‐G^N438A expresses VP2 gene‐encoded IPNV (infectious pancreatic necrosis virus) against both pathogens in rainbow trout