Oral Delivery of Purple Sweet Potato (Ipomoea batatas L.) Extract-Loaded Carboxymethyl Chitosan and Alginate Nanocapsule in Streptozotocininduced Diabetic Mice

Abstract

Background: Insulin therapy is an essential part in diabetes mellitus type 2 treatment, but it has several side effects such as allergy and hypoglycemia. Therefore, alternative treatments are needed, one of which is by using nanocapsules to increase the performance of the drug delivery system. Purple sweet potato contain high anthocyanin levels which has antidiabetic properties. The idea of this research were to determine the potential use of purple sweet potato extract-loaded CMC-Alginate nanocapsules and to address problems regarding the efficiency of encapsulation to increase the bioavailability. Methods: This experimental research used post-test only control group design. The samples were 24 mice which were separated into four groups based on the administration of the following, respectively: 0.5 mL of extract-containing CMC-Alginate nanocapsules, 0.5 mL of extract, 1 mL of glibenclamide as the positive control and 0.5 mL of 0.5% CMC sodium placebo as the negative control. Mice were conditioned to be diabetic by induction of Streptozotocin. Blood glucose levels were carried out on days 1, 3, and 7 using an "Easy Touch" glucometer. The statistical analysis was conducted by two-way ANOVA proceeded by Tukey's post hoc test to investigate the differences between all groups. Results: Statistically, purple sweet potato extract-loaded CMC-Alginate nanocapsules, which its extract concentration was 4.4 times less than that of extract without encapsulation, can reduce high glucose levels in mice when as compared to negative control (p<0.05). Conclusion: Purple sweet potato extract-loaded CMC-Alginate nanocapsules has capability to reduce blood glucose levels of streptozotocininduced mice.

Combination of purple sweet potato (Ipomoea batatas L.) leaf extract with metformin on blood glucose and total cholesterol levels of albino rats induced by high-fat diet and streptozotocin

“…13,16 Anthocyanins are flavonoid phytopigments with antioxidant properties. 17 Previous research has shown that EDUJ is anti-hyperglycaemic by stimulating the secretion of glucagon-like peptide-1 (GLP-1), the antidiabetic mechanism of anthocyanins by stimulating GLP-1, increatin hormone, and triggering the proliferation of insulin hormone and pancreatic β-cells. 10,17 Saponins have hypoglycemic activity and prevent diabetic complications due to their antioxidant activity.…”

Nanoformulations for the Delivery of Dietary Anthocyanins for the Prevention and Treatment of Diabetes Mellitus and Its Complications

“…More recently, I’tishom et al [ 115 ] showed that DM can be treated with carboxymethyl chitosan and alginate nanocapsules loaded with anthocyanin-rich purple-sweet-potato-extract. Researchers found that purple sweet potato extract-loaded chitosan and alginate nanocapsules were 4.4 times more effective at lowering blood glucose levels in diabetic mice caused by Streptozotocin than the extract alone.…”

Antidiabetic properties of analog rice from local raw materials: a systematic review

“…Besides that, anthocyanin can trigger a potent incretin hormone, also known as glucagon-like peptide-1 (GLP-1). This hormone can stimulate insulin secretion through proliferation and help to keep pancreatic βcells [67], [68].…”