Oral delivery of nanoparticles - let’s not forget about the protein corona

Berardi, Alberto; Bombelli, Francesca Baldelli

doi:10.1080/17425247.2019.1610384

Cited by 51 publications

(32 citation statements)

References 24 publications

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“…Then, protein corona was formed by injecting 1% FBS onto the immobilized NPs. When APOH was added to SNPs with protein corona in different concentrations (1,5,15,50, and 150 µg ml −1 ), a fraction of APOH remained associated with SNPs in a concentration-dependent manner (Fig. 4h).…”

Section: Sc/hc Show Different Binding States Of the Same Proteinsmentioning

confidence: 96%

“…Upon exposure to biofluids, the NPs acquire a "protein corona" due to the adherence of host proteins on the NPs surface. The composition of the corona is dependent on the types of nanoparticles and the biological sources [1][2][3] , and considered to provide the NPs with a "biological" identity 4 that affects stability, circulation time, and cellular uptake/interactions, and therefore has a strong impact on the functional role for the NPs [5][6][7][8][9][10] .…”

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confidence: 99%

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Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

et al. 2020

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The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identify a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins are revealed as modulators of nanoparticle-cell association mainly through their dynamic nature. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

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Section: Sc/hc Show Different Binding States Of the Same Proteinsmentioning

confidence: 96%

mentioning

confidence: 99%

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

et al. 2020

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“…As the particles emerge from the stomach they will be rapidly neutralized by pancreatic secretions, which again may cause a change in the enzymatic corona governed presumably by pancreatic enzymes. Using polymer nanoparticles it has been shown that the formation of an enzymatic corona occurs after exposure to digestive enzyme which may affect their delivery properties . In future work it will be necessary to understand the dynamic properties of the gastrointestinal enzymatic corona as well as to undertake systematic studies on the stability of the pre‐incubated hard protein coronas within the gastrointestinal tract with a specific focus on the formation of an enzymatic corona.…”

Section: Resultsmentioning

confidence: 99%

Influence of a Protein Corona on the Oral Pharmacokinetics of Testosterone Released from Mesoporous Silica

Giri

Kuschnerus

Ruan

et al. 2019

Advanced Therapeutics

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The formation of a protein corona adsorbed on mesoporous silica particles with 2D and 3D pore structures after incubation in fetal bovine serum is investigated. The compositional properties of the resulting protein-particle complex is determined by using mass spectrometry as a function of serum incubation time. The effect of the adsorbed proteins on the drug release properties of the mesoporous particles when these are used to deliver the hormone TST is examined in dissolution media. Changes to the pharmacokinetic parameters are validated in vivo in a rat model. Small changes to the maximum plasma concentration, terminal half-life, and area under the plasma concentration time curve are observed with pre-incubation of a hard protein corona around the particles. This strategy is used to slow down the release of pharmaceutical drugs loaded onto mesoporous silica, and acts as a formulation strategy for future nanomedical applications involving advanced drug delivery.

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“…Then, protein corona was formed by injecting 1 % FBS onto the immobilized NPs (shown in supplementary Fig.15). Subsequently, APO H was injected in different concentrations ( 1,5,15,50, and 150 µg/ml) (h). Two-dimensional fits were applied to the data to calculate K d and K off values for different populations of APO H binding to the SNPs@HC (i).…”

Section: Fig 1 Spaac Click Chemistry Reaction and Characterization mentioning

confidence: 99%

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Mohammad‐Beigi

Hayashi

Zeuthen

et al. 2020

Preprint

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The current understanding of the biological identity that nanoparticles may acquire in a given biological milieu is mostly inferred from the hard component of the protein corona (HC). The composition of soft corona (SC) proteins and their biological relevance have remained elusive due to the lack of analytical separation methods. Here, we identified a set of specific corona proteins with weak interactions at silica and polystyrene nanoparticles by using an in situ click-chemistry reaction. We show that these SC proteins are present also in the HC, but are specifically enriched after the capture, suggesting that the main distinction between HC and SC is the differential binding strength of the same proteins. Interestingly, the weakly interacting proteins in the SC are revealed as modulators of nanoparticle-cell association, in spite of their short residence time. We therefore highlight that weak interactions of proteins at nanoparticles should be considered when evaluating nano-bio interfaces.

show abstract

Oral delivery of nanoparticles - let’s not forget about the protein corona

Cited by 51 publications

References 24 publications

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

Influence of a Protein Corona on the Oral Pharmacokinetics of Testosterone Released from Mesoporous Silica

Mapping and identification of soft corona proteins at nanoparticles and their impact on cellular association

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