Oral delivery of dsRNA by microbes: Beyond pest control

Abrieux, Antoine; Chiu, Joanna C.

doi:10.1080/19420889.2016.1236163

Cited by 19 publications

(15 citation statements)

References 25 publications

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“…As an attractive alternative, the use of oral delivery of dsRNA by microbes (biopesticides) has been proposed . This approach employs free‐living microorganisms, including model organisms, such as Saccharomyces cerevisae , to deliver dsRNA to control insects .…”

Section: Discussionmentioning

confidence: 99%

“…A similar approach could be used to modify plant bacterial endophytes to express dsRNA targeting insect pests, as described for Bt toxins . Oral delivery of dsRNA by microbes offers a great potential to be a part of integrated pest management, contributing to overcoming limitations from genetic modification of plants …”

Section: Discussionmentioning

confidence: 99%

“…11,59 As an attractive alternative, the use of oral delivery of dsRNA by microbes (biopesticides) has been proposed. 60 This approach employs free-living microorganisms, including model organisms, such as Saccharomyces cerevisae, to deliver dsRNA to control insects. 61 Mass production of bacteria expressing dsRNA to be used as an oral delivery system costs less than synthetic or in vitro synthesized dsRNA, and it can be easily applied to crops.…”

Section: Wileyonlinelibrarycom/journal/psmentioning

confidence: 99%

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Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

Bento

Marques

Campana

et al. 2019

Pest Management Science

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BACKGROUND RNA interference (RNAi) has been evaluated in several insect pests as a novel strategy to be included in integrated pest management. Lepidopterans are recognized to be recalcitrant to gene silencing by RNAi. As such, double‐stranded RNA (dsRNA) delivery needs to be adjusted to assure its stability until it reaches the target gene transcript for silencing. Gene silencing by RNAi offers the potential to be used in the control of Tuta absoluta (Meyrick), one of the main insect pests of tomato (Solanum lycopersicum) worldwide. Here, we tested the delivery of dsRNA expressed in Escherichia coli HT115(DE3) and supplied to larvae in an artificial diet by screening target genes for silencing. We tested six target genes: juvenile hormone inducible protein (JHP); juvenile hormone epoxide hydrolase protein (JHEH); ecdysteroid 25‐hydroxylase (PHM); chitin synthase A (CHI); carboxylesterase (COE); and arginine kinase (AK). RESULTS Based on larval mortality, the duration of the larval stage in days, pupal weight, and the accumulation of the target gene transcript, we demonstrated the efficacy of bacterial dsRNA delivery for the functional effects on larval development. Providing dsRNA targeted to JHP, CHI, COE and AK by bacteria led to a significant decrease in transcript accumulation and an increase in larval mortality. CONCLUSION Bacteria expressing dsRNA targeting essential T. absoluta genes supplied in artificial diet are efficient to screen RNAi target‐genes. The oral delivery of dsRNA by bacteria is a novel potential alternative for the control of T. absoluta based on RNAi. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Wileyonlinelibrarycom/journal/psmentioning

confidence: 99%

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Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

Bento

Marques

Campana

et al. 2019

Pest Management Science

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“…Recently, a study showed that significant gene knockdown was achieved when yeast cells expressing dsRNA were fed to Drosophila suzukii (Abrieux & Chiu, ; Murphy, Tabuloc, Cervantes, & Chiu, ). Cellular uptake of orally delivered dsRNA may also be improved by encapsulating dsRNA in nanomaterials such as branched amphiphilic peptide capsules (Avila et al, ) or engineered ribonucleoprotein particles (Gillet et al, ).…”

Section: Discussionmentioning

confidence: 99%

Lethal RNA interference response in the pepper weevil

Taylor

Pinheiro

et al. 2019

J Applied Entomology

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The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae) is an important pest of peppers (Capsicum spp.) in the southern United States, Mexico, and Central America. Current management practices rely on chemical control of adults although grower options are limited. A more integrated strategy to manage the pepper weevil infestations may benefit from measures that can control this pest specifically such as RNA interference (RNAi). We cloned and sequenced transcript fragments of two possible essential genes of the pepper weevil, Snf7 and V‐ATPase‐A. We treated pepper weevil females with the double‐stranded RNAs (dsRNAs) of these genes and found that orally delivered dsRNA of either gene was not effective in eliciting strong RNAi response or causing mortality. In contrast, Snf7 and V‐ATPase‐A dsRNAs delivered through injections produced robust gene silencing and led to more than 90% mortality within 12 days. Our results suggest that the pepper weevil possesses the molecular machinery needed for a robust RNAi response and that Snf7 and V‐ATPase‐A genes likely encode essential functions in this insect. Moreover, RNAi‐mediated silencing of essential genes can potentially be used to control this pest if strong RNAi response can be induced by orally delivered dsRNA.

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“…Current methods to control D. suzukii rely considerably on the use of expensive, broad-spectrum insecticides (e.g., malathion), which have variable efficacy 5 , are difficult to use due to timing of fruit infestation 7 , and face the risk of D. suzukii evolving resistance 8 . While other forms of control may be possible (e.g., the use of recently identified natural predators 9 or oral delivery of dsRNA by microbes 10 ), these approaches have not been widely adopted 11,12 . Therefore, given the rapid worldwide spread and potential economic impact of D. suzukii, novel effective control measures are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Synthetically EngineeredMedeaGene Drive System in the Worldwide Crop Pest,D. suzukii

Buchman

Marshall

Ostrovski

et al. 2017

Preprint

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Synthetic gene drive systems possess enormous potential to replace, alter, or suppress wild populations of significant disease vectors and crop pests; however, their utility in diverse populations remains to be demonstrated. Here, we report the creation of the first-ever synthetic Medea gene drive element in a major worldwide crop pest, D. suzukii. We demonstrate that this drive element, based on an engineered maternal "toxin" coupled with a linked embryonic "antidote," is capable of biasing Mendelian inheritance rates with up to 100% efficiency.However, we find that drive resistance, resulting from naturally occurring genetic variation and associated fitness costs, can hinder the spread of such an element. Despite this, our results suggest that this element could maintain itself at high frequencies in a wild population, and spread to fixation, if either its fitness costs or toxin resistance were reduced, providing a clear path forward for developing future such systems.

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Oral delivery of dsRNA by microbes: Beyond pest control

Cited by 19 publications

References 25 publications

Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

Lethal RNA interference response in the pepper weevil

Synthetically EngineeredMedeaGene Drive System in the Worldwide Crop Pest,D. suzukii

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