Oral delivery of DNA vaccine encoding VP28 against white spot syndrome virus in crayfish by attenuated Salmonella typhimurium

Ning, Jian; Zhu, Wei; Xu, Jianqing; Zheng, Cong; Meng, Xiao

doi:10.1016/j.vaccine.2008.11.075

Cited by 62 publications

(34 citation statements)

References 42 publications

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“…Recently, it reported that ORF 53R targeted artificial miRNAs that could mediate iridovirus resistance to RGV infection by specific silencing pathway [33]. The envelope protein is reported to be an effective vaccine against WSSV [10,34]. There is a general agreement that anti-envelope antibodies that bind to the envelope spike on the virion are neutralized or show antiviral activity [35].…”

Section: Discussionmentioning

confidence: 99%

Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Xiong¹,

Dong²,

Weng³

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Xiong¹,

Dong²,

Weng³

et al. 2011

Fish & Shellfish Immunology

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“…Attenuated S. typhimurium as a gene delivery vector provides more advantages when compared to viral vectors, such as good targeting. [24][25][26] For example, Salmonella can: (i) specifically colonize in hypoxic tumor tissues if administered through the intravenous or intraperitoneal routes; (ii) be used across a wide range of tissues; (iii) express foreign genes in any hypoxic region, unlike a retroviral vector which can only infect dividing cells; (iv) proliferate in vivo and efficiently express exogenous gene products; and by itself exerts antitumor effects; (v) be an ideal and safe vehicle for gene transfer since that it is sensitive to antibiotics and small doses of currently available antibiotics can kill it; and (vi) not be rejected by the host upon longterm use. Taken together, these characteristics make S. typhimurium an ideal vector for gene therapy.…”

Section: Introductionmentioning

confidence: 99%

Plasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth

Liu

Zhang²,

Guo³

et al. 2012

Asian J Androl

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Persistent activation of Survivin and its overexpression contribute to the formation, progression and metastasis of several different tumor types. Therefore, Survivin is an ideal target for RNA interference mediated-growth inhibition. Blockade of Survivin using specific short hairpin RNAs (shRNA) can significantly reduce prostate tumor growth. RNA interference does not fully ablate target gene expression, owing to the idiosyncrasies associated with shRNAs and their targets. To enhance the therapeutic efficacy of Survivin-specific shRNA, we employed a combinatorial expression of Survivin-specific shRNA and gene associated with retinoid-interferon-induced mortality-19 (GRIM-19). Then, the GRIM-19 coding sequences and Survivin-specific shRNAs were used to create a dual expression plasmid vector and were carried by an attenuated strain of Salmonella enteric serovar typhimurium (S. typhimurium) to treat prostate cancer in vitro and in vivo. We found that the co-expressed Survivin-specific shRNA and GRIM-19 synergistically and more effectively inhibited prostate tumor proliferation and survival, when compared with treatment with either single agent alone in vitro and in vivo. This study has provided a novel cancer gene therapeutic approach for prostate cancer.

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“…Numerous studies have explored different vaccination strategies to protect shrimp from WSSV infection, including inactivated WSSV vaccines (45), recombinant protein-based vaccines (46,47), DNA-based vaccines (48)(49)(50), and RNA-based vaccines (51)(52)(53)(54)(55)(56)(57). VP28, which is the most abundant WSSV envelope protein, is the major target for vaccine design, and oral vaccination is thought to be the most practical route for vaccine delivery (58).…”

Section: Discussionmentioning

confidence: 99%

VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection

Han

et al. 2016

J Virol

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Oral ingestion is the major route of infection for the white spot syndrome virus (WSSV). However, the mechanism by which virus particles in the digestive tract invade host cells is unknown. In the present study, we demonstrate that WSSV virions can bind to chitin through one of the major envelope proteins (VP24). Mutagenesis analysis indicated that amino acids (aa) 186 to 200 in the C terminus of VP24 were required for chitin binding. Moreover, the P-VP24 186 -200 peptide derived from the VP24 chitin binding region significantly inhibited the VP24-chitin interaction and the WSSV-chitin interaction, implying that VP24 participates in WSSV binding to chitin. Oral inoculation experiments showed that P-VP24 186 -200 treatment reduced the number of virus particles remaining in the digestive tract during the early stage of infection and greatly hindered WSSV proliferation in shrimp. These data indicate that binding of WSSV to chitin through the viral envelope protein VP24 is essential for WSSV per os infection and provide new ideas for preventing WSSV infection in shrimp farms. IMPORTANCEIn this study, we show that WSSV can bind to chitin through the envelope protein VP24. The chitin-binding domain of VP24 maps to amino acids 186 to 200 in the C terminus. Binding of WSSV to chitin through the viral envelope protein VP24 is essential for WSSV per os infection. These findings not only extend our knowledge of WSSV infection but also provide new insights into strategies to prevent WSSV infection in shrimp farms. W hite spot syndrome virus (WSSV) is an enveloped doublestranded DNA (dsDNA) virus belonging to the genus Whispovirus, family Nimaviridae (1). WSSV has caused enormous economic losses to the shrimp farming industry since 1993. Infectious WSSV virions are typically 250 to 380 nm in length and 120 to 150 nm in diameter (2, 3), and they contain a genome of ϳ300 kb that encodes ϳ180 proteins (4, 5).Ingestion of WSSV-infected sick or dead shrimp has been accepted as the major route of natural infection due to the cannibalistic nature of shrimp (6-11). Therefore the digestive tract of shrimp may be a primary site of infection. The digestive tract of shrimp is composed of the esophagus, stomach, midgut, and hindgut. The esophagus, stomach, and hindgut possess a chitinous lining, which is not present in the midgut (12, 13). Instead, the midgut epithelium is generally lined with the peritrophic membrane (PM), which is a noncellular structure surrounding the food bolus. The PM is composed of regularly arranged chitin fibrils embedded in a matrix of proteins, proteoglycans, and mucopolysaccharides (14-17). Therefore, WSSV must cross the PM in the midgut or the chitinous lining in the other parts of the digestive tract to traverse the basal membranes and reach the host cells. We speculate that the interaction between WSSV and chitin may be important for WSSV infection in the shrimp digestive tract.More than 40 WSSV structural proteins have been identified using proteomic methods to date (18)(19)(20). Among them, VP28,...

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Oral delivery of DNA vaccine encoding VP28 against white spot syndrome virus in crayfish by attenuated Salmonella typhimurium

Cited by 62 publications

References 42 publications

Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus

Plasmid-based Survivin shRNA and GRIM-19 carried by attenuated Salmonella suppresses tumor cell growth

VP24 Is a Chitin-Binding Protein Involved in White Spot Syndrome Virus Infection

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