Oral contrast enhances the resolution of in-life NIS reporter gene imaging

Suksanpaisan, Lukkana; Pham, Linh; McIvor, Scott; Russell, Stephen J.; Peng, Kah-Whye

doi:10.1038/cgt.2013.57

Cited by 12 publications

(17 citation statements)

References 8 publications

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“…[ 46 ] The use of contrast agents can further increase the ability to differentiate organs by blocking endogenous uptake prior to administration of the radiotracer. [ 54 ]…”

Section: Detection Of Viral Replication and Biodistributionmentioning

confidence: 99%

The use of the NIS reporter gene for optimizing oncolytic virotherapy

Miller

Russell

2015

Expert Opinion on Biological Therapy

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Introduction: Oncolytic viruses are experimental cancer therapies being translated to the clinic. They are unique in their ability to amplify within the body, therefore requiring careful monitoring of viral replication and biodistribution. Traditional monitoring strategies fail to recapitulate the dynamic nature of oncolytic virotherapy. Consequently, clinically relevant, noninvasive, high resolution strategies are needed to effectively track virotherapy in real time. Areas covered: The expression of the sodium iodide symporter (NIS) reporter gene is tightly coupled to viral genome replication and mediates radioisotope concentration, allowing noninvasive molecular nuclear imaging of active viral infection with high resolution. This provides insight into replication kinetics, biodistribution, the impact of vector design, administration, and dosing on therapeutic outcomes, and highlights the heterogeneity of spatial distribution and temporal evolution of infection. NIS-mediated imaging in clinical trials confirms the feasibility of this technology to noninvasively and longitudinally observe oncolytic virus infection, replication, and distribution. Expert opinion: NIS-mediated imaging provides detailed functional and molecular information on the evolution of oncolytic virus infection in living animals. The use of NIS reporter gene imaging has rapidly advanced to provide unparalleled insight into the spatial and temporal context of oncolytic infection which will be integral to optimization of oncolytic treatment strategies.

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“…[ 46 ] The use of contrast agents can further increase the ability to differentiate organs by blocking endogenous uptake prior to administration of the radiotracer. [ 54 ]…”

Section: Detection Of Viral Replication and Biodistributionmentioning

confidence: 99%

The use of the NIS reporter gene for optimizing oncolytic virotherapy

Miller

Russell

2015

Expert Opinion on Biological Therapy

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“…Coregistration of the SPECT and CT images was performed by the application of precalibrated spatial transformation to the SPECT images to match the CT images. In order to mask background signals from the stomach, animals were administered 350 μL of undiluted barium sulfate (40% wt/vol; Tagitol V, E‐Z‐EM, Lake Success, NY) by oral gavage with a 22‐gauge plastic feeding tube (Instech Laboratories, Plymouth, PA), as previously described …”

Section: Methodsmentioning

confidence: 99%

“…In order to mask background signals from the stomach, animals were administered 350 lL of undiluted barium sulfate (40% wt/vol; Tagitol V, E-Z-EM, Lake Success, NY) by oral gavage with a 22-gauge plastic feeding tube (Instech Laboratories, Plymouth, PA), as previously described. 24…”

Section: Spect/ct Imaging Systemmentioning

confidence: 99%

Noninvasive 3‐dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene

et al. 2015

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4 Imanis Life Sciences, Rochester, MNCell transplantation is a potential treatment for the many liver disorders that are currently only curable by organ transplantation. However, one of the major limitations of hepatocyte (HC) transplantation is an inability to monitor cells longitudinally after injection. We hypothesized that the thyroidal sodium iodide symporter (NIS) gene could be used to visualize transplanted HCs in a rodent model of inherited liver disease: hereditary tyrosinemia type 1. Wild-type C57Bl/6J mouse HCs were transduced ex vivo with a lentiviral vector containing the mouse Slc5a5 (NIS) gene controlled by the thyroxine-binding globulin promoter. NIS-transduced cells could robustly concentrate radiolabeled iodine in vitro, with lentiviral transduction efficiencies greater than 80% achieved in the presence of dexamethasone. Next, NIS-transduced HCs were transplanted into congenic fumarylacetoacetate hydrolase knockout mice, and this resulted in the prevention of liver failure. NIStransduced HCs were readily imaged in vivo by single-photon emission computed tomography, and this demonstrated for the first time noninvasive 3-dimensional imaging of regenerating tissue in individual animals over time. We also tested the efficacy of primary HC spheroids engrafted in the liver. With the NIS reporter, robust spheroid engraftment and survival could be detected longitudinally after direct parenchymal injection, and this thereby demonstrated a novel strategy for HC transplantation. This work is the first to demonstrate the efficacy of NIS imaging in the field of HC transplantation. We anticipate that NIS labeling will allow noninvasive and longitudinal identification of HCs and stem cells in future studies related to liver regeneration in small and large preclinical animal models. Liver Transpl 21:442-453, 2015. V C 2015 AASLD.

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“…Although NanoSPECT-CT (Mediso Medical Imaging Systems, Budapest, Hungary) of gavaged technetium-labeled activated charcoal diethylene triaminepentaacetic acid has been used to assess gastrointestinal transit in mice, 26 to our knowledge SPECT imaging has not been used to assess gastric accommodation specifically in nonhuman animals. Given the rapidly advancing use of 99m Tc-pertechnetate and other sodium/iodide symporter substrates in numerous animal models 27 , 28 as well as descriptions of methods to circumvent the high stomach signal that confounds such studies, 29 it is reasonable to assume that this well-validated approach to assess gastric accommodation in human beings can be reverse-translated easily for use in preclinical studies.…”

Section: Tests To Evaluate Gastric Capacity and Accommodationmentioning

confidence: 99%

Measurement of Gastrointestinal and Colonic Motor Functions in Humans and Animals

Camilleri

Linden

2016

Cellular and Molecular Gastroenterology and Hepatology

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Synopsis Accurately measuring the complex motor behaviors of the gastrointestinal tract has tremendous value for the understanding, diagnosis and treatment of digestive diseases. This review synthesizes the literature regarding current tests that are used in both humans and animals. There remains further opportunity to enhance such tests, especially when such tests are able to provide value in both the preclinical and the clinical settings.

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Oral contrast enhances the resolution of in-life NIS reporter gene imaging

Cited by 12 publications

References 8 publications

The use of the NIS reporter gene for optimizing oncolytic virotherapy

The use of the NIS reporter gene for optimizing oncolytic virotherapy

Noninvasive 3‐dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene

Measurement of Gastrointestinal and Colonic Motor Functions in Humans and Animals

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