Oral Branched‐Chain Amino Acids Decrease Whole‐Body Proteolysis

Ferrando, Arny A.; Williams, Brandon; Stuart, Charles Alexander; Lane, Helen W.; Wolfe, Robert R.

doi:10.1177/014860719501900147

Cited by 64 publications

(39 citation statements)

References 18 publications

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“…The third reason may be the extra-protein effect of the BCAA blend (Ferrando et al, 1995;Anthony et al, 2000). There are lots of researches related to the extra-protein effect of the BCAA and/or a role for BCAA in protein synthesis (Anthony et al, 2000;Ospina-Rojas et al, 2014), although these effect and roles of the BCAA blend were not determined in the present study.…”

Section: Resultsmentioning

confidence: 70%

Growth, Digestive Tract and Muscle Weights in Slow-Growing Broiler is not Affected by a Blend of Branched-Chain Amino Acids Injected into Different Sites of Egg

2015

JAES

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One hundred-fifty fertile eggs from 30-week-old Ross x Rhode Island Red (RIR) breeders as a factorial arrangement of 3 injection site (amnion, yolk sac or albumin) and 2 levels of BCAA (Control and BCAA, 0.2%) were arranged into 6 groups with 3 replicates. The solutions used in ovo feeding were aseptically prepared to contain 0% (C) or 0.2% BCAA (mixed within ratio of 2 L-leucine: 1 L-valine: 1 L-isoleucine) in 0.5% saline. At 18 d of incubation 1 ml of in ovo feeding solution was injected into the amnion (A), yolk sac (Y) or albumin (AL) of egg. Hence, there were six nutrition treatments; AC (n = 25), ABCAA (n = 25), YC (n = 25), YBCAA (n = 25), ALC (n = 25) and ALBCAA (n = 25). Upon hatch obtained birds were fed on standard broiler diets until slaughter (8 weeks of age). The YC had lower gizzard weight (P = 0.023) compared to other treatments. The interaction effect of factors on the gizzard weight was found significant (P < 0.025). The in ovo BCAA injection increased the gizzard weight of AL chicks while decreased that of A and Y birds. The crude fat content of thigh meat from Y birds was lower than those of A and AL broilers (P = 0.029). However, growth performance, muscles weights, digestive tract weight and length and abdominal fat, hearth, liver, weights are not affected by factors. The role of in ovo BCAA injection in slow-growing broiler needs further research.

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Section: Resultsmentioning

confidence: 70%

Growth, Digestive Tract and Muscle Weights in Slow-Growing Broiler is not Affected by a Blend of Branched-Chain Amino Acids Injected into Different Sites of Egg

2015

JAES

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“…Four diets composed of purified 20% vitamin-free casein diet with (1) or without (2) vitamin B6 (7.0 mg of pyridoxine HCl/kg of diet) and with (1) Isolation of lipoproteins and purification of apo E. Isolation and purification of VLDL (d,1.006 g/mL) using preparative ultracentrifugation from pooled serum of male Wistar rats and delipidation of VLDL were performed as described previously (16)(17)(18). Delipidated VLDL (40-45 mg) was solubilized in 6 m guanidine HCl and 0.05 m Tris-HCl (pH 8.4) containing 0.1% NaN3 and was applied on Sepharose CL-6B.…”

Section: Methodsmentioning

confidence: 99%

“…Branched-chain amino acids (BCAAs) composed of valine, leucine, and isoleucine are essential amino acids and account for about 40% of dietary essential amino acids in body protein and about 15% of total amino acids in muscle proteins (1). BCAAs play a role in delayed onset of muscle soreness and fatigue, and suppression of fatigue in the central nervous system (2,3).…”

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confidence: 99%

High Incidence of Lipid Deposition in the Liver of Rats Fed a Diet Supplemented with Branched-Chain Amino Acids under Vitamin B6 Deficiency

Kaimoto

Shibuya

Nishikawa

et al. 2013

J Nutr Sci Vitaminol

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73Branched-chain amino acids (BCAAs) composed of valine, leucine, and isoleucine are essential amino acids and account for about 40% of dietary essential amino acids in body protein and about 15% of total amino acids in muscle proteins (1). BCAAs play a role in delayed onset of muscle soreness and fatigue, and suppression of fatigue in the central nervous system (2, 3). BCAAs have been also used for the prevention of hepatic encephalopathy in patients with liver cirrhosis (4, 5). Recently, sport enthusiasts have created an increased demand for dietary supplements with BCAAs and have increased their consumption of BCAAs-enriched soft drinks.The first step in the catabolism of BCAAs involves branched-chain aminotransferase (BCAT; EC 2.6.1.42), a common enzyme. This enzyme requires vitamin B 6, which functions as a cofactor in many enzyme reactions of amino acid metabolism (6, 7). However, little information is available on the relationship between supplementation with BCAAs and the recommended daily allowance of vitamin B6. In addition, it is known that a high-protein diet (e.g., 70% casein diet) under vitamin B6 deficiency has a negative impact on several biochemical parameters and leads to fatty liver in rats (8-10).Our previous study showed that providing supplemented BCAAs under vitamin B6 deficiency and a 20% casein diet caused an accumulation of triacylglycerol in the liver, and individual differences in biological responses were observed (11). It is possible that a disorder of amino acid metabolism in the presence of vitamin B6 deficiency might influence the assembly of lipids and apolipoproteins and/or the secretion of lipoproteins. Among the serum lipoproteins, very low-density lipoprotein (VLDL) is present during endogenous lipogenesis in the liver and its major apolipoproteins are apolipoprotein (apo) B, apo C, and apo E (12). It is interesting to compare serum apo B and apo E levels, and hepatic apoE levels to evaluate lipid deposition, because apo B and apo E are key determinants responsible for cellular recognition and internalization of chylomicron remnants, VLDL, and high-density lipoprotein (HDL) (13,14). In this study, we investigated the possible mechanism of lipid deposition in the liver of rats fed with the supplementation of BCAA under vitamin B 6 deficiency and suggested the presence of impaired secretion of VLDL into the bloodstream from the liver. Materials and MethodsDiets and animals. Four diets composed of purified 20% vitamin-free casein diet with (1) or without (2) vitamin B6 (7.0 mg of pyridoxine HCl/kg of diet) and with (1) or without (2) Summary Male Wistar rats were fed four diets composed of purified 20% vitamin-free casein diet with (1) or without (2) vitamin B6 (7.0 mg of pyridoxine HCl/kg of diet) and with (1) or without (2) branched-chain amino acids (BCAAs) of valine, leucine, and isoleucine (4.75%): B6 (1)BCAA (2); B6(1)BCAA(1); B6 (2)BCAA (2); and B6 (2)BCAA (1) for 21 d. Among rats fed the B6(2)BCAA(1) diet, about a half showed lipid deposition in the liver. On the other...

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“…An overnight infusion of BCAA was associated with a 20-60% decline in arterial concentrations of the other amino acids. This suggested that the BCAA inhibit proteolysis in skeletal muscle and other body tissues (Ferrando et al, 1995;Louard et al, 1995). In addition to the protein sparing effects, leucine and the other BCAA exert their effects on respiratory functions.…”

Section: Branched Chain Amino Acids (Valine Leucine Isoleucine)mentioning

confidence: 99%

A review of physiological and metabolic effects of essential amino acids

1998

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The authors review ten essential amino acids with regard to their metabolic, physiologic and therapeutic effects throughout the human body. Physical properties of these biologically active compounds are discussed as a foundation for their diverse roles in special nitrogen containing products, neurotransmitters, and as alternative energy sources. Both normal and abnormal amino acid metabolism are considered in the areas of digestion, elimination of metabolic products, metabolic intermediates, and defects in these systems. Recent developments in therapeutic applications are further examined for clinical utility and as an economical alternative to traditional clinical treatment modalities.

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Oral Branched‐Chain Amino Acids Decrease Whole‐Body Proteolysis

Abstract: These results suggest that branched-chain amino acid ingestion suppresses whole-body proteolysis in tissues other than skeletal muscle in normal men.

Cited by 64 publications

References 18 publications

Growth, Digestive Tract and Muscle Weights in Slow-Growing Broiler is not Affected by a Blend of Branched-Chain Amino Acids Injected into Different Sites of Egg

Growth, Digestive Tract and Muscle Weights in Slow-Growing Broiler is not Affected by a Blend of Branched-Chain Amino Acids Injected into Different Sites of Egg

High Incidence of Lipid Deposition in the Liver of Rats Fed a Diet Supplemented with Branched-Chain Amino Acids under Vitamin B6 Deficiency

A review of physiological and metabolic effects of essential amino acids

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