Oral arginine improves intestinal recovery following ischemia-reperfusion injury in rat

Sukhotnik, Igor; Helou, Habib; Mogilner, Jorge G.; Lurie, M.; Bernsteyn, Aleksander; Coran, Arnold G.; Shiloni, Eitan

doi:10.1007/s00383-004-1318-0

Cited by 48 publications

(39 citation statements)

References 21 publications

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“…The less significant damage to the proximal jejunum may have been due to blood flow from the celiac axis. Our data show that programmed cell death is one of the pathways leading to mucosal damage, which is consistent with data from our previous work [25] and with data reported by other investigators [15,16]. Both decreased cell proliferation and increased cell apoptosis suggest decreased enterocyte turnover, which might explain the mucosal hypoplasia after IR.…”

Section: Discussionsupporting

confidence: 94%

Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat

et al. 2007

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The aim of the present study was to evaluate the preventive effect of a 2-day oral glutamine supplementation against intestinal ischemia-reperfusion (IR) injury in a rat. Male Sprague-Dawley rats were divided into four experimental groups: sham rats underwent laparotomy, sham-GLU rats underwent laparotomy and were treaded with enteral glutamine (GLU) given in drinking water (2%) 48 hr before and following operation, IR rats underwent occlusion of both the superior mesenteric artery and the portal vein for 30 min followed by 24 hr of reperfusion, and IR-GLU rats were treated with enteral glutamine 48 hr before and following IR. Intestinal mucosal damage (Park's injury score), mucosal structural changes, enterocyte proliferation, and enterocyte apoptosis were determined 24 hr following IR. Sham-GLU rats demonstrated a lower rate of cell apoptosis in jejunum and ileum compared to sham animals. IR-GLU animals demonstrated a greater jejunal and ileal bowel and mucosal weight, mucosal DNA, villous height and crypt depth, and enterocyte proliferation index in ileum and a lower injury score grade in jejunum compared to IR-nontreated rats. In conclusion, pretreatment with oral glutamine prevents mucosal injury and improves intestinal recovery following IR injury in the rat.

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Section: Discussionsupporting

confidence: 94%

Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat

et al. 2007

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“…Judging from these results, apoptosis might be induced in the bottom of villi after I/R as a pathological condition. Previous studies evaluated mucosal damage with a decrease in villous height and an increase in injury score in I/R injury in the intestine (4,21,27). In this study, I/R induced marked destruction of structure with mucosal erosion and edema with a decrease in villous height, but the site of apoptosis after I/R was not identified because of mucosal damage.…”

Section: Discussioncontrasting

confidence: 52%

Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion

Fujise

Iwakiri²,

Wu³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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We have previously demonstrated that fasting and ischemia-reperfusion (I/R) induced apoptosis in rat intestinal mucosa. It is widely accepted that apoptosis is induced through two main pathways. This study aimed to compare apoptotic pathways following fasting and I/R. Rats were divided into two groups: the I/R group involved occlusion of the superior mesenteric artery for 60 min, followed by 60-min reperfusion, whereas the fasting group involved fasting for 24 or 48 h. Intestinal apoptosis was assessed as percentage of fragmented DNA, by electrophoresis and by a terminal deoxynucleotidyl transferase mediated dUDP-biotin nick- end labeling (TUNEL) assay. Apoptotic proteins including death ligands/receptors and caspases were evaluated by Western blot analysis. Small intestinal mucosal height and mitochondrial dehydrogenase function were assessed. Fasting and I/R significantly induced intestinal apoptosis. Mucosal height was significantly decreased in fasting rats, and mitochondrial dysfunction was induced only by I/R. Expressions of Fas, Fas ligand, and TNF-alpha type 1 receptor were enhanced in fasting and I/R rats. After I/R, expressions of cytochrome c and cleaved caspase-9 were significantly increased. In contrast, expressions of cleaved caspase-8 and cleaved caspase-3 increased in fasting rats. Fasting promoted mucosal apoptosis via a receptor-mediated type I apoptotic pathway in the rat small intestine, and I/R induced apoptosis via a mitochondria-mediated type II pathway.

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“…NO was reported to play an important role in damaging the testis in I/R [7] . Conversely, NO was reported to be cytoprotective in testicular I/R injury [18][19][20][21] . Increased expression of the iNOS, eNOS and neuronal NOS in the testis after I/R was demonstrated [22] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Curcumin on Ipsilateral and Contralateral Testes after Unilateral Testicular Torsion in a Rat Model

Başaran¹,

Dökmeci²,

Yalçın³

et al. 2008

Urol Int

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Objective: The aim of the study was to determine the protective effect of curcumin on testicular ischemia-reperfusion (I/R) injury. Materials and Methods: 32 male rats were divided into four groups (n = 8): group 1: control; group 2: ischemia; group 3: I/R, and group 4: I/R+CUR. Curcumin (150 mg/kg, p.o.) was administered before 30 min of reperfusion in group 4. Malondialdehyde (MDA) levels, Johnsen’s testicular biopsy scores, and mean seminiferous tubule diameter measurements were evaluated in testes. In addition, endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expressions were evaluated immunohistochemically. Results: MDA levels in control groups were significantly lower than other groups in ipsilateral and contralateral testes. Johnsen’s scores in the control group were significantly higher than in other groups. MDA levels and Johnsen’s scores in the I/R+CUR group were similar to the ischemia and I/R groups in ipsilateral and contralateral testes. The immunoreactivity of iNOS and eNOS were increased in I/R ipsilateral testicular groups. After I/R, iNOS and eNOS expression increased slightly in contralateral groups. Additionally, the curcumin treatment decreased iNOS and eNOS immunoreactivity in ipsilateral and contralateral testes. Conclusion: The results suggest that curcumin did not protect the unilateral nor contralateral testes. This observation may depend on inhibition of iNOS and eNOS due to inhibition of the antioxidant, anti-inflammatory effects of nitric oxide.

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Oral arginine improves intestinal recovery following ischemia-reperfusion injury in rat

Cited by 48 publications

References 21 publications

Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat

Dietary Glutamine Supplementation Prevents Mucosal Injury and Modulates Intestinal Epithelial Restitution Following Ischemia-Reperfusion Injury in the Rat

Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion

Effect of Curcumin on Ipsilateral and Contralateral Testes after Unilateral Testicular Torsion in a Rat Model

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