Oral and Fecal Microbiota in Lynch Syndrome

Ferrarese, Roberto; Zuppardo, Raffaella Alessia; Puzzono, Marta; Mannucci, Alessandro; Amato, Virginia; Ditonno, Ilaria; Patricelli, Maria Grazia; Raucci, Annalisa Russo; Clementi, Massimo; Elmore, Ugo; Rosati, Riccardo; Testoni, Pier Alberto; Mancini, Nicasio; Cavestro, Giulia Martina

doi:10.3390/jcm9092735

Cited by 12 publications

(8 citation statements)

References 44 publications

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“…Interestingly, colonization of colibactin-producing E. coli was demonstrated in 15% of stool samples of three-day neonates, suggesting a potential role in eoCRC pathogenesis [ 220 ]. As a distinct microbial signature has recently been identified in Lynch syndrome [ 221 , 222 ], future large studies should analyze the role of microbiota in eoCRC pathogenesis, focusing also on the role of colibactin-producing E. coli associated with different dietary habits.…”

Section: Discussionmentioning

confidence: 99%

The Role of Diet and Lifestyle in Early-Onset Colorectal Cancer: A Systematic Review

Puzzono

Mannucci

Grannò

et al. 2021

Cancers

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The incidence of early-onset colorectal cancer, defined as colorectal cancer occurring in young adults under the age of 50, is increasing globally. Knowledge of the etiological factors in young adults is far from complete. Questionable eoCRCs’ exogenous factors are represented by processed meat, sugary drinks, alcohol, Western dietary pattern, overweight and obesity, physical inactivity, and smoking, though with heterogeneous results. Therefore, we performed a systematic review to summarize the current evidence on the role of diet and lifestyle as eoCRC risk factors. We systematically searched PubMed, Scopus, and EMBASE up to July 2021, for original studies evaluating diet, alcohol, physical activity, BMI, and smoking in eoCRC and included twenty-six studies. Indeed, the exogenous factors could represent modifiable key factors, whose recognition could establish areas of future interventions through public health strategies for eoCRC primary prevention. Additionally, we discussed the role of additional non-modifiable risk factors, and of epigenetic regulation and microbiota as mediators of the eoCRC triggered by diet and lifestyle.

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Section: Discussionmentioning

confidence: 99%

The Role of Diet and Lifestyle in Early-Onset Colorectal Cancer: A Systematic Review

Puzzono

Mannucci

Grannò

et al. 2021

Cancers

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“…These swabs were selected because of their use in previous studies, easy accessibility, and low cost ( Kaan et al., 2020 ; Susic et al., 2020 ). In addition, we tested three different methods that include DNA extraction and host DNA depletion in comparison with the QIAamp PowerFecal DNA kit, which contains no host DNA depletion step, as reference standard used in many microbiome projects with similar respiratory and vaginal samples ( Bartolomaeus et al., 2021 ; Boeck et al., 2017 ; Ferrarese et al., 2020 ; Lebeer et al., 2018 ; Lim et al., 2020 ). First, we tested host DNA depletion by osmotic lysis of mammalian cells and treatment with propidium monoazide (PMA), prior to lysis of the microbial cells and DNA extraction using the QIAamp PowerFecal DNA kit, as described by Marotz et al.…”

Section: Introductionmentioning

confidence: 99%

Microbial enrichment and storage for metagenomics of vaginal, skin, and saliva samples

et al. 2021

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Summary Few validated protocols are available for large-scale collection, storage, and analysis of microbiome samples from the vagina, skin, and mouth. To prepare for a large-scale study on the female microbiome by remote self-sampling, we investigated the impact of sample collection, storage, and host DNA depletion on microbiome profiling. Vaginal, skin, and saliva samples were analyzed using 16S rRNA gene amplicon and metagenomic shotgun sequencing, and qPCR. Of the two tested storage buffers, the eNAT buffer could keep the microbial composition stable during various conditions. All three tested host DNA-depletion approaches showed a bias against Gram-negative taxa. However, using the HostZERO Microbial DNA and QIAamp DNA Microbiome kits, samples still clustered according to body site and not by depletion approach. Therefore, our study showed the effectiveness of these methods in depleting host DNA. Yet, a suitable approach is recommended for each habitat studied based on microbial composition.

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“…The following primers: V3-16S-Fw: CCTACGGGNGGCWGCAG and V4-16S-Rev: GACTACHVGGGTATCTAATCC were used. The ampli condition was 94°C for 2 min, 35 cycles of 94°C for 30 s, 56°C for 30 s, 68°C for 1 min, and kept at 4°C[59, 60]. The amplicon was sequenced using Illumina MiSeq by Shanghai Paisano Biological Technology Co., LTD.The Quantitative Insights into Microbial Ecology version 2 (QIIME2) was used for quality ltering of DNA sequences, demultiplexing, taxonomic assignment, and calculating β-diversity.…”

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Effects of Salmonella enteritidis Infection on TLR Genes Expression and Microbial Diversity in Laying Hens

Jiang

Zhang

Sun

et al. 2022

Preprint

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Background: Salmonella enteritidis (SE) is one of the critical foodborne pathogens, and mainly causes human diseases through contaminated water and food. In the current study, serological tests, histopathological observation and quantitative real-time PCR were used to assess the effect of Salmonella enteritidis infection on the immune system in chickens. To understand the influence of SE infection on the microflora profile of the cecum and oviduct, microbial community composition of the cecal contents and oviducal contents wereanalyzed through 16S rRNA sequencing. Results: Results showed that SEinfection caused damage to the digestive organs, reproductive organs, and immune organs in laying hens. The expression of TLR1a, TLR1b, TLR2, TLR4, TLR5, TLR7 and TLR15 in the cecum were induced, and the content of IFN-γ, TNF-α, IL-2 and IL-18 in serum were increased after SE infection. The composition of the microbial community significantly changed in cecal content, the dominant bacteria of Firmicutes increased, and Bacteroidetes decreased significantly. In the oviduct, the microbial diversity became complicated, the dominant bacteria Faecalibacterium was significantly increased, and Bacteroides was significantly decreased. Conclusions: Salmonella enteritidis infection triggered the host’s innate and adaptive response, increased the expression of TLRs and contents of cytokines and changed the microbial diversity in the cecum and oviduct. These findings willlay the foundation for further study on reducing the vertical transmission of Salmonella enteritidis, ensuring the production of clean eggs.

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Oral and Fecal Microbiota in Lynch Syndrome

Cited by 12 publications

References 44 publications

The Role of Diet and Lifestyle in Early-Onset Colorectal Cancer: A Systematic Review

The Role of Diet and Lifestyle in Early-Onset Colorectal Cancer: A Systematic Review

Microbial enrichment and storage for metagenomics of vaginal, skin, and saliva samples

Effects of Salmonella enteritidis Infection on TLR Genes Expression and Microbial Diversity in Laying Hens

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